A coupling coup: A novel microfluidic chip with an integrated nanospray emitter enables the first dead‐volume‐free coupling of glass‐chip laboratories with mass spectrometry. An electrophoretic version of the system may be suitable for the separation and analysis of drugs, for example, and has potential for the high‐throughput analysis of miniscule amounts of samples.
A new spin: The addition of chemically inert perfluoro carboxylic acids (green; see picture) to P450 enzymes results in dramatic activation of their catalytic activity as a result of the conversion of the Fe/heme from a low‐spin to a high‐spin state, and the reduction of the binding‐pocket size. Together these effects allow otherwise inert substrates such as propane and even methane to be oxidized.
Ein neuer Dreh: Die Zugabe chemisch inerter Perfluorcarbonsäuren (grün im Bild) zu P450‐Enzymen erhöht deren katalytische Aktivität drastisch. Gründe sind der Übergang des Fe/Häms von einem Low‐ in einen High‐Spin‐Zustand und die Verkleinerung der Bindungstasche. In der Summe hat das zur Folge, dass sonst inerte Substrate wie Propan und sogar Methan oxidiert werden.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.