Microfluidic fabrication of water-in-water droplets encapsulated in hydrogel microfibers

Liu, Chenguang; Zheng, Wenxu; Xie, Ruoxiao; Liu, Yupeng; Liang, Zhe; Luo, Guangsheng; Ding, Mingyu; Liang, Qionglin

doi:10.1016/j.cclet.2018.09.010

Cited by 28 publications

(15 citation statements)

References 39 publications

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“…Also, the mechanical valves within the microfluidic device can facilitate the stable and controllable generation of droplet templates in an all-in-water microenvironment, avoiding the utilization of the oil phase that may lead to potential cytotoxicity. [17][18][19] In addition, compared to the passive microfluidic flow focusing platform, 33 our valve-based platform can significantly increase the uniformity and throughput of waterin-water droplets. This improvement is beneficial for the controllable and massive generation of organoids from hiPSCs.…”

Section: Design and Manipulation Of The Oil-free Droplet Microfluidic Platformmentioning

confidence: 99%

“…The reagents used in these systems, such as the oil phase and/or surfactants, may reduce the biocompatibility of hydrogel capsules, leading to potential protein or enzyme denaturation, inhibition of cell growth, and limited mass transfer in subsequent cell culture. [17][18][19] Alternatively, the aqueous two-phase system (ATPS) has provided an oil-free manner in droplet microfluidic systems for the fabrication of biomimetic and/or cell-laden hydrogel scaffolds. [20][21][22][23][24][25] Hydrogel capsules generated in such systems can be solidified under moderate conditions, and are potential scaffolds for cell assembly and 3D culture.…”

Section: Introductionmentioning

confidence: 99%

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One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs

et al. 2020

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Section: Design and Manipulation Of The Oil-free Droplet Microfluidic Platformmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs

et al. 2020

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“…Meanwhile, the microfluidic approach has been regularly used to fabricate cell-laden microfibers (Table 1). [10][11][12][13][14][15][16] Typically, single and coaxial microfluidic devices enable fabrication of various cell-laden microfibers with precisely controlled cell distribution structures such as droplets, [10] knots, [11][12][13] double layers, [14] hollow structuring, [15] and coiled spring structuring, [16] with optimization for the intended application. Cell-laden microfibers with specific cell distribution structures exhibit high cell viability (>85%).…”

Section: Introductionmentioning

confidence: 99%

A Microfluidic Device to Fabricate One‐Step Cell Bead‐Laden Hydrogel Struts for Tissue Engineering

Kim

Lee

Jin

et al. 2021

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been used as 3D tissue structures in newly discovered drugs and personalized medicines and are more relevant to in vivo microenvironmental conditions than 2D substrates. [2] Recently, a variety of micro/nanoscale biofabrication methods have been used to build complex 3D biomedical structures. Unlike conventional processes, which can incur unpredictable properties, such as non-homogeneous physicochemical and mechanical properties, within the structure, 3D bioprinting provides precise control of various biophysical and biochemical properties. It can also be used to print different cell types that are appropriate to the region of tissue restoration.However, despite advances in 3D bioprinting of scaffolds for various tissue engineering applications, developing functional bioprinting of cell-laden structures remains challenging due to several limitations. A common shortcoming of bioprinted structures is the low degree of cell-cell interaction in matrix hydrogels, which are critical for tissue restoration. Additionally, relatively high cell densities in bioink can cause significant cell damage during the printing process. Even fabricated structures with high cell densities cannot sustain printed 3D structures due to their relatively low physical strength.However, although the appropriate cell density for a given application is highly dependent on the cell type and hydrogel physicochemical properties, high cell density induces efficient cell-to-cell signaling and regulates stem cell differentiation. [3][4][5] According to Maia et al., high densities of human mesenchymal stem cells cultured in Arg-Gly-Asp (RGD)-alginate 3D matrixes allow the development of multicellular structures and effectively stimulates osteogenic differentiation of the implanted cells. [6] Thus, cell spheroids, high density 3D cell aggregations, can be considered microtissues that mimic natural microenvironmental conditions by providing sufficient cell-to-cell or cell-to-ECM interactions to increase various cellular activities, including cell differentiation. [7] In general, spheroids are used to treat or regenerate damaged tissues through an injection process. However, to obtain the intricately designed configurations necessary for some applications, spheroids must be mixed with hydrogels and spheroid-laden bioink printed into 3D mesh structures.Cell-laden structures are widely applied for a variety of tissue engineering applications, including tissue restoration. Cell-to-cell interactions in bioprinted structures are important for successful tissue restoration, because cell-cell signaling pathways can regulate tissue development and stem cell fate. However, the low degree of cell-cell interaction in conventional cellladen bioprinted structures is challenging for the therapeutic application of this modality. Herein, a microfluidic device with cell-laden methacrylated gelatin (GelMa) bioink and alginate as a matrix hydrogel is used to fabricate a functional hybrid structure laden with cell-aggregated microbeads. This approach effectively increases t...

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“…Fiber‐shaped materials can be produced by solidifying the microflows. Gas, oil, water droplets or cell spheroids can be combined into flows to generate microfiber‐based materials with enriched functions and expanded applications in multiple fields.…”

Section: Engineering Approaches On Fabricating Perfusable Microchannementioning

confidence: 99%

Engineering of Hydrogel Materials with Perfusable Microchannels for Building Vascularized Tissues

Xie

Zheng

Guan

et al. 2019

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Vascular systems are responsible for various physiological and pathological processes related to all organs in vivo, and the survival of engineered tissues for enough nutrient supply in vitro. Thus, biomimetic vascularization is highly needed for constructing both a biomimetic organ model and a reliable engineered tissue. However, many challenges remain in constructing vascularized tissues, requiring the combination of suitable biomaterials and engineering techniques. In this review, the advantages of hydrogels on building engineered vascularized tissues are discussed and recent engineering techniques for building perfusable microchannels in hydrogels are summarized, including micromolding, 3D printing, and microfluidic spinning. Furthermore, the applications of these perfusable hydrogels in manufacturing organ‐on‐a‐chip devices and transplantable engineered tissues are highlighted. Finally, current challenges in recapitulating the complexity of native vascular systems are discussed and future development of vascularized tissues is prospected.

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Microfluidic fabrication of water-in-water droplets encapsulated in hydrogel microfibers

Cited by 28 publications

References 39 publications

One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs

One-step synthesis of composite hydrogel capsules to support liver organoid generation from hiPSCs

A Microfluidic Device to Fabricate One‐Step Cell Bead‐Laden Hydrogel Struts for Tissue Engineering

Engineering of Hydrogel Materials with Perfusable Microchannels for Building Vascularized Tissues

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