Microfluidic electrochemical assay for rapid detection and quantification of Escherichia coli

Disposable Biosensors for Clinical Diagnosis

“…Disposable electrochemical sensors and biosensors have significant applications as diagnostic tools in the point-of-care testing. The use of several disposable biosensors in the point-ofcare assessment of acetylcholinesterase, 127 bacteria, [128][129][130][131] cancer biomarkers, [132][133][134] and DNA targets 135 has been reported in the literature. A portable sensor system comprising sixteen electrodes for DNA detection is illustrated in Figure 9.…”

“…Different strategies have been proposed for the detection of cardiac biomarkers under the concept of point-of-care diagnostics. [129][130][131][132][133][134][135] For example, Jagadeesan et al have designed a low-cost and rapid method for the modification of paper electrodes with conducting polymers for the amperometric detection of troponin 138 for point-of-care diagnosis. Polyaniline was deposited on the patterned screen printed paper electrodes by electrodeposition, followed by immobilization of anti-cardiac troponin-I using 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride and N -hydroxysuccinimide coupling.…”

A novel, sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes

“…Despite the requirement of extensive effort in designing six sets of primers, LAMP can be easily integrated with lab on a chip devices to perform the detection process, which clearly applicable in the development of biosensor and POC monitoring devices (Mori et al, 2001., Notomi et al, 2000. LAMP amplicon were detected utilizing turbidity (Mori et al, 2001), electrochemical (Ahmed et al, 2009, Safavieh et al 2012a, gel electrophoresis (Le et al, 2012), fluorescence (Hong et al, 2012, Safavieh et al, 2012b, Safavieh et al,2014a, lateral flow test and magnetic beads (Roy, S et al, 2016). All these detection modalities have a major drawback in providing significant difference between positive and negative samples, which yields difficulties in quantitative analysis of nucleic acids.…”

A sensitive impedance biosensor based on immunomagnetic separation and urease catalysis for rapid detection of Listeria monocytogenes using an immobilization-free interdigitated array microelectrode

“…As an alternative, various biosensors, including Surface Plasmon Resonance (SPR) (Karoonuthaisiri et al, 2014), Quartz Crystal Microbalance(QCM) (Su and Li., 2005), optics (Massad-Ivanir et al, 2013) and electrochemistry (Safavieh et al, 2012), etc., have been developed for the detection of foodborne pathogens. Impedance biosensor is a typical electrochemical biosensor, generally relying on the measurement of the electrochemical impedance change on the interface of an electrode under an alternating-current potential with a direct-current bias.…”