A novel, sensitive and label-free loop-mediated isothermal amplification detection method for nucleic acids using luminophore dyes

Loop‐Mediated Isothermal Amplification (LAMP): A Rapid and Sensitive Tool for Quality Assessment of Meat Products

“…Various compounds are used as the luminophore; however, the cation of tris(bipyridine)ruthenium(II) chloride [Ru(bpy) 3 ]Cl 2 , that is [Ru(bpy) 3 ] 2+ , shows strong luminophore characteristics and provides higher chemical stability in the aqueous solutions with better emission characteristics (Caspar and Meyer ). In a novel detection method, the LAMP technique was coupled with ECL for the detection and quantification of NA (Roy and others ). This solution‐based immobilization‐free LAMP‐ECL method was completed using [Ru(bpy) 3 ]Cl 2 as a luminophore, and tripropylamine as a co‐reactant with the target DNA.…”

Trends and Advances in Electrochemiluminescence Nanobiosensors

“…ECL has diverse analytical applications, such as identifying co-reactants, ECL enhancers and ECL quenchers, or used in biomolecules detection, food and drug analysis, clinical diagnosis and environmental monitoring due to its high sensitivity, broad linear range and rapid analysis. ECL has also been coupled to other analytical techniques, such as flow injection analysis, high-performance liquid chromatography (HPLC), capillary electrophoresis, and micro total analysis (mTAS) [ 7 , 8 , 15 , 41 , 42 , 43 ]. The widespread use of ECL biosensors for biomolecule detection in various fields in the last decade has seen numerous adaptations incorporated in the fabrication of ECL biosensors, specifically to modify electrodes, ECL labels and ECL-emitting probes with different NSMs or NMs or MNCs and their composites.…”

“…Figure 1 specifically shows the number of research articles that have been published on electrochemiluminescence (ECL) biosensors in the last 10 years. Some of the important applications of biosensors include medical diagnosis [ 6 , 7 , 8 , 9 , 10 , 11 ], the food industry [ 12 , 13 , 14 , 15 , 16 , 17 ], biomedicine [ 18 ], forensic science [ 19 , 20 , 21 ] environmental monitoring [ 16 , 22 ], water characteristic testing [ 23 ], defense and the military [ 24 ], as well as security [ 25 ]. In real-world settings, biosensors have replaced many tedious, expensive and complex conventional analytical techniques.…”

“…The selection of the suitable biomolecule immobilization method is usually followed by deciding on the transducer for an effective bio/immunosensor. A transducer is a component which converts chemical [ 6 , 31 ], optical [ 7 , 15 ], mass [ 39 ] or temperature [ 40 ] signal into an electrical signal (see Table 1 ). Henceforth, depending on the types of transducer or the signal transduced, biosensors are mainly divided into electrochemical, optical, thermal, and piezoelectric devices.…”

Paper microchip with a graphene-modified silver nano-composite electrode for electrical sensing of microbial pathogens

“…Recently, various classes of paper- and plastic-based materials have opened a new paradigm in developing a wide range of low-cost and disposable biosensing devices with significant potential for field applications in resource-limited settings. 4–9 Such devices are easy-to-fabricate, mass-producible, disposable, and inexpensive and can be integrated with various detection modalities, such as fluorescence, 10–12 electrochemical, 13–15 photoelectrochemical, 16 electrochemiluminescence 17, 18 and colorimetric. 19–21 …”