Microfluidic DNA microarray analysis: A review

Wang, Lin; Li, Paul C. H.

doi:10.1016/j.aca.2010.11.056

Cited by 131 publications

(78 citation statements)

References 142 publications

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“…Due to their miniaturization and automation, there are a number of advantages of using microfluidic systems, such as less sample/reagent consumption, reduced risk of contamination, less cost per analysis, lower power consumption, enhanced sensitivity and specificity, and higher reliability. Microfluidic systems have been developed for various biological analytical applications, such as DNA analysis [4][5][6][7][8], immunoassay [9][10][11][12][13], and cell analysis [14][15][16][17][18]. Moreover, a number of demonstrations showed that cell culture can be performed on the microfluidic systems to achieve higher throughput and more reliable results [19,20].…”

Section: Introductionmentioning

confidence: 99%

Review on Impedance Detection of Cellular Responses in Micro/Nano Environment

Lei

2014

Micromachines

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Abstract:In general, cell culture-based assays, investigations of cell number, viability, and metabolic activities during culture periods, are commonly performed to study the cellular responses under various culture conditions explored. Quantification of cell numbers can provide the information of cell proliferation. Cell viability study can understand the percentage of cell death under a specific tested substance. Monitoring of the metabolic activities is an important index for the study of cell physiology. Based on the development of microfluidic technology, microfluidic systems incorporated with impedance measurement technique, have been reported as a new analytical approach for cell culture-based assays. The aim of this article is to review recent developments on the impedance detection of cellular responses in micro/nano environment. These techniques provide an effective and efficient technique for cell culture-based assays.

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Section: Introductionmentioning

confidence: 99%

Review on Impedance Detection of Cellular Responses in Micro/Nano Environment

Lei

2014

Micromachines

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“…In the search for more powerful analytical tools, planar microarray technology go beyond since it can be easily adapted to high throughput tests, show multiplex capabilities and allow quantitative analysis with high potential applicability in life sciences [15][16][17]. In this line, arrays on compact disks have shown great potential when they are applied in the areas of food safety and environmental analysis [18,19].…”

Section: Introductionmentioning

confidence: 99%

Arrays on disc for screening and quantification of pollutants

Navarro

Morais

Gabaldón

et al. 2013

Analytica Chimica Acta

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Morais Ezquerro, SB.; Gavaldón, JA.; Pérez, AJ.; Puchades Pla, R.; Maquieira Catala, Á. (2013). Arrays on disc for screening and quantification of pollutants. Analytica Chimica Acta. 784:59-64. doi:10.1016/j.aca.2013.04.041. Document downloaded from:This paper must be cited as:The final publication is available at Author's personal copy Analytica Chimica Acta 784 (2013) [59][60][61][62][63][64] Contents lists available at SciVerse ScienceDirect Analytica Chimica Acta • Disc-arrays were developed for quantitative determination of pollutants.• Dozens of samples were analyzed in 40 min after simple dilution.• High-throughput multiplexed screening of organic contaminants at a very competitive value.• User-friendly and robust methodology with high applicability for monitoring programs, in situ analysis, in low-level labs or outside the lab setting. g r a p h i c a l a b s t r a c t a r t i c l e i n f o a b s t r a c tA rapid compact disc based methodology for screening and quantification of organic pollutants in mandarin juices is presented. The assay is established on the coating conjugate indirect competitive principle and developed under disc-array configuration. The detection is based on the acquisition of attenuated reflective signals that were proportional to optical density of the immunoreaction product. The competitive assay is applied to quantify simultaneously, in a selective manner, non-systemic insecticides in mandarin juices. The detection limits were 0.2 and 0.1 g L −1 and the sensitivity 2.1 and 1.5 g L −1 , for chlorpyrifos and fenthion, respectively. Pollutants were directly quantified after sample dilution in a total time of 40 min. Also, the implementation of positive and negative controls into the array configuration served as an automatic quality control test. The effect of thermal treatment on pesticide dissipation was studied and found that it was insignificant under the studied conditions. Recovery intervals ranged from 96-105% to 94-103%, for chlorpyrifos and fenthion, respectively and were similar to those obtained with gas chromatography coupled to mass spectrometry. In the current configuration, 64 samples can be simultaneously analyzed on a disc at a very competitive value, demonstrating its potential for highthroughput multiplexed screening applications for controlled monitoring programs in low-level labs or outside the lab setting.

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“…[10][11][12][13][14][15][16][17] These platforms adopt miniaturized, multiplexed and parallel processing and detection methods, and several technologies that immobilize surface binding molecules, such as DNA [18][19][20][21][22] or protein, [6][7][8][9]23,24 have been successfully developed. The platforms developed to date have achieved reductions in cost while excluding complex instrumentation for multiplexed bioassays.…”

Section: Introductionmentioning

confidence: 99%

Fiber composite slices for multiplexed immunoassays

et al. 2015

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Fabrication methods for the development of multiplexed immunoassay platforms primarily depend on the individual functionalization of reaction chambers to achieve a heterogeneous reacting substrate composition, which increases the overall manufacturing time and cost. Here, we describe a new type of low-cost fabrication method for a scalable immunoassay platform based on cotton threads. The manufacturing process involves the fabrication of functionalized fibers and the arrangement of these fibers into a bundle; this bundle is then sectioned to make microarray-like particles with a predefined surface architecture. With these sections, composed of heterogeneous thread fragments with different types of antibodies, we demonstrated quantitative and 7-plex immunoassays. We expect that this methodology will prove to be a versatile, low-cost, and highly scalable method for the fabrication of multiplexed bioassay platforms. V C 2015 AIP Publishing LLC. [http://dx

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Microfluidic DNA microarray analysis: A review

Cited by 131 publications

References 142 publications

Review on Impedance Detection of Cellular Responses in Micro/Nano Environment

Review on Impedance Detection of Cellular Responses in Micro/Nano Environment

Arrays on disc for screening and quantification of pollutants

Fiber composite slices for multiplexed immunoassays

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