Microfluidic Devices for Cell, Tissue and Embryo Culture

Ibrahim, Muhammad

doi:10.2174/22102965113039990019

Cited by 4 publications

(8 citation statements)

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“…Microfluidic culture of endothelial cells is currently an emerging technology which mimics the physiological shear stress on cultured cells to achieve the goal of culturing functional blood vessels for tissue engineering [13,99]. A number of techniques for culturing vascular networks have been described in which endothelial growth factors, ECM components and microfluidics are combined (see following sections for detailed discussion);however, the development of fully functional blood vessels still remains a challenge [99].…”

Section: Haemodynamic Factorsmentioning

confidence: 99%

“…They contain hollow channels that can be filled with ECM components and medium [13]. The devices can simply be connected to a medium reservoir to generate a passive flow through the channels; or to a pump for more constant flow rate ( Table 5).…”

Section: Beyond Organoids: Microfluidics and The Development Of A Funmentioning

confidence: 99%

“…For further discussion of microfluidic devices in the field of vascular culture, see Refs. [13,99,256].…”

Section: Examples Of Microfluidic Vascular Culture Systemsmentioning

confidence: 99%

“…Similarly, masses of normal (non-malignant) cells grown in vitro are called organoids, and are currently the focus of great interest in biomedical research because they show some organization of tissues resembling in vivo organs [12]. We believe that the development of anin vitro vascular network could improve the culturing of spheroids and organoids ( Figure 1) by allowing the tissues to grow and function in a way that is closer to the in vivosituation [13].…”

Section: Introductionmentioning

confidence: 99%

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Beyond organoids: In vitro vasculogenesis and angiogenesis using cells from mammals and zebrafish

Ibrahim

Richardson

2017

Reproductive Toxicology

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The ability to culture complex organs is currently an important goal in biomedical research. It is possible to grow organoids (3D organ-like structures) in vitro; however, a major limitation of organoids, and other 3D culture systems, is the lack of a vascular network. Protocols developed for establishing in vitro vascular networks typically use human or rodent cells. A major technical challenge is the culture of functional (perfused) networks. In this rapidly advancing field, some microfluidic devices are now getting close to the goal of an artificially perfused vascular network. Another development is the emergence of the zebrafish as a complementary model to mammals. In this review, we discuss the culture of endothelial cells and vascular networks from mammalian cells, and examine the prospects for using zebrafish cells for this objective. We also look into the future and consider how vascular networks in vitro might be successfully perfused using microfluidic technology.

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Section: Haemodynamic Factorsmentioning

confidence: 99%

Section: Beyond Organoids: Microfluidics and The Development Of A Funmentioning

confidence: 99%

“…For further discussion of microfluidic devices in the field of vascular culture, see Refs. [13,99,256].…”

Section: Examples Of Microfluidic Vascular Culture Systemsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Beyond organoids: In vitro vasculogenesis and angiogenesis using cells from mammals and zebrafish

Ibrahim

Richardson

2017

Reproductive Toxicology

Self Cite

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“…Microfluidic devices can be very helpful as platforms for mammalian cell culture because they allow an accurate control over the local microenvironment, and they greatly reduce the volume of costly reagents required [66,67]. By using microfluidic devices for cell culture purposes, more accurate in vitro results can be obtained since living cells can also be considered as microscale systems.…”

Section: Developing Novel Microfluidic Devices and Microenvironments mentioning

confidence: 99%

Integrative Utilization of Microenvironments, Biomaterials and Computational Techniques for Advanced Tissue Engineering

Shamloo

Mohammadaliha

Mina

2015

Journal of Biotechnology

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In vitro development of zebrafish vascular networks

Ibrahim

Richardson

2017

Reproductive Toxicology

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A major limitation to culturing tissues and organs is the lack of a functional vascular network in vitro. The zebrafish possess many useful properties which makes it a promising model for such studies. Unfortunately, methods of culturing endothelial cells from this species are not well characterised. Here, we tried two methods (embryoid body culture and organ explants from transgenic zebrafish kdrl:GFP embryos) to develop in vitro vascular networks. In the kdrl:GFP line, endothelial cells expresses green fluorescent protein, which allows to track the vascular development in live cultures. We found that embryoid bodies showed significantly longer and wider branches of connected endothelial cells when grown in a microfluidic system than in static culture. Similarly, sprouting of kdrl:GFP cells from the tissue explants was observed in a 3D hydrogel matrix. This study is a step towards the development of zebrafish vascular networks in vitro.

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Microfluidic Devices for Cell, Tissue and Embryo Culture

Cited by 4 publications

References 0 publications

Beyond organoids: In vitro vasculogenesis and angiogenesis using cells from mammals and zebrafish

Beyond organoids: In vitro vasculogenesis and angiogenesis using cells from mammals and zebrafish

Integrative Utilization of Microenvironments, Biomaterials and Computational Techniques for Advanced Tissue Engineering

In vitro development of zebrafish vascular networks

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