In vitro development of zebrafish vascular networks

Ibrahim, Muhammad; Richardson, Michael K.

doi:10.1016/j.reprotox.2017.02.008

Cited by 5 publications

(11 citation statements)

References 77 publications

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“…The development of these cells into vascular networks in response to various signals could then be readily tracked in live cultures without the need to fix and stain them. Furthermore, tissues and organ explants from zebrafish embryos can be a promising model for sprouting angiogenesis, as we have shown using liver and heart explants from 5 dpf embryos [173].…”

Section: Cultures Of Specialised Zebrafish Cell Typesmentioning

confidence: 90%

“…Explant cultures are usually established in a 3D gel matrix in the presence of angiogenic growth factors, and are examined for microvessel outgrowth (vascular sprouting) [127,173].…”

Section: Use Of Tissue Explants For In Vitro Angiogenesismentioning

confidence: 99%

“…To harvest sterile cells or tissues from zebrafish embryos it is necessary to decontaminate the surface of the chorion. Using this approach, it is possible to isolate and culture sterile cells from blastula (3 hpf) or gastrula (24 hpf) stage embryos [209,212].In a recent study, we have shown that embryos with a chorion decontaminated at 24 hpf could be further cultured to 5 dpfunder aseptic conditions [173]. The tissues and cells isolated from these embryos were successfully maintained free of contamination for eight days in culture.…”

Section: Cell Culture Techniques In Zebrafishmentioning

confidence: 99%

“…Strategies have also been described for the culture of primary neurons from developing brain and spinal cord cells of zebrafish embryos [223]. We have found that the endothelial cells in dissociated hearts, isolated from 5 dpf zebrafish embryos,form colonies ona fibronectin substratum; however, these cultures could only be maintained for short periods, possibly because of low seeding-density [173].…”

Section: Cultures Of Specialised Zebrafish Cell Typesmentioning

confidence: 99%

“…(D) In this device, endothelial cells line the built-in channels cut into a hydrogel with embedded supporting cells [248,249]. (E) A microfluidic channel slide with a 3D matrix plug (P) in the middle of the channel [173]. The medium flows on either side of the plug (a small amount may penetrate the gel by diffusion).…”

Section: Examples Of Microfluidic Vascular Culture Systemsmentioning

confidence: 99%

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Beyond organoids: In vitro vasculogenesis and angiogenesis using cells from mammals and zebrafish

Ibrahim

Richardson

2017

Reproductive Toxicology

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The ability to culture complex organs is currently an important goal in biomedical research. It is possible to grow organoids (3D organ-like structures) in vitro; however, a major limitation of organoids, and other 3D culture systems, is the lack of a vascular network. Protocols developed for establishing in vitro vascular networks typically use human or rodent cells. A major technical challenge is the culture of functional (perfused) networks. In this rapidly advancing field, some microfluidic devices are now getting close to the goal of an artificially perfused vascular network. Another development is the emergence of the zebrafish as a complementary model to mammals. In this review, we discuss the culture of endothelial cells and vascular networks from mammalian cells, and examine the prospects for using zebrafish cells for this objective. We also look into the future and consider how vascular networks in vitro might be successfully perfused using microfluidic technology.

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Section: Cultures Of Specialised Zebrafish Cell Typesmentioning

confidence: 90%

“…Explant cultures are usually established in a 3D gel matrix in the presence of angiogenic growth factors, and are examined for microvessel outgrowth (vascular sprouting) [127,173].…”

Section: Use Of Tissue Explants For In Vitro Angiogenesismentioning

confidence: 99%

Section: Cell Culture Techniques In Zebrafishmentioning

confidence: 99%

Section: Cultures Of Specialised Zebrafish Cell Typesmentioning

confidence: 99%

Section: Examples Of Microfluidic Vascular Culture Systemsmentioning

confidence: 99%

See 3 more Smart Citations

Beyond organoids: In vitro vasculogenesis and angiogenesis using cells from mammals and zebrafish

Ibrahim

Richardson

2017

Reproductive Toxicology

Self Cite

View full text Add to dashboard Cite

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Microfluidics: Recent Advances Toward Lab‐on‐Chip Applications in Bioanalysis

Sharma

2021

Adv Eng Mater

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Microfluidics is an emerging technology of flow and control of fluids in microscale volume that offers highly specialized solutions to rapid, sensitive, noninvasive analysis and measurements in various engineering applications. When combined with microelectromechanical systems (MEMSs), microfluidics technology has shown remarkable developments in small‐scale devices for biosensing, chemical solutions, and precise detection of particles at a higher rate. MEMS‐inspired microfluidics technology can contribute to the highly sensitive, recyclable, and portable sensing platforms for large‐scale integration devices. In this review, the history and developmental phases occurring in MEMS are detailed. Finally, this review is concluded with future scope and guidelines for enabling the various approaches to overcome the obstacles and the mass commercialization of microfluidic devices. Recent applications of microfluidics in biosensing are also highlighted where research should be focused in the future.

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