Microfluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure

Lim, Jaewoo; Kang, Byunghoon; Son, Ho‐Young; Mun, Byeonggeol; Huh, Yong Min; Rho, Hyun Wook; Kang, Taejoon; Moon, Jeong Hee; Lee, Jaejong; Seo, Seung Beom; Jang, Soojin; Son, Seong Uk; Jung, Juyeon; Haam, Seungjoo; Lim, Eun‐Kyung

doi:10.1016/j.bios.2021.113753

Cited by 40 publications

(18 citation statements)

References 52 publications

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“…Lim et al, developed a system that integrates a microfluidic chip, 3D-nanostructured hydrogels and exosomatic mRNA sensors. The system can further detect the exosomal ERBB2 in the blood associated with breast cancer, thus proving the validity of the system in the diagnosis of breast cancer [169]. Table 5 depicts the overview of applications of microfluidic POC devices in the early diagnosis of tumors (cancer).…”

Section: Applications For Cancer Detectionmentioning

confidence: 94%

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Yang

Zhang

et al. 2022

Sensors

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The early diagnosis of infectious diseases is critical because it can greatly increase recovery rates and prevent the spread of diseases such as COVID-19; however, in many areas with insufficient medical facilities, the timely detection of diseases is challenging. Conventional medical testing methods require specialized laboratory equipment and well-trained operators, limiting the applicability of these tests. Microfluidic point-of-care (POC) equipment can rapidly detect diseases at low cost. This technology could be used to detect diseases in underdeveloped areas to reduce the effects of disease and improve quality of life in these areas. This review details microfluidic POC equipment and its applications. First, the concept of microfluidic POC devices is discussed. We then describe applications of microfluidic POC devices for infectious diseases, cardiovascular diseases, tumors (cancer), and chronic diseases, and discuss the future incorporation of microfluidic POC devices into applications such as wearable devices and telemedicine. Finally, the review concludes by analyzing the present state of the microfluidic field, and suggestions are made. This review is intended to call attention to the status of disease treatment in underdeveloped areas and to encourage the researchers of microfluidics to develop standards for these devices.

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Section: Applications For Cancer Detectionmentioning

confidence: 94%

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Yang

Zhang

et al. 2022

Sensors

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“…In a recent work, Lim and colleagues developed a microfluidic platform for mRNA detection in EVs from blood of HER2 + breast tumor bearing mice in 2 h. 69 The exoNA-sensing chip comprised a sensing chamber composed of 3D-nanostructured hydrogels with fluorescent probes for the detection of ERBB2 and GADPH reference gene. In resting conditions, the 3D-hydrogel loaded with probe-bearing liposomes did not produce any fluorescent signal (Fig.…”

Section: A Colorimetric Detectionmentioning

confidence: 99%

Microfluidic platforms for extracellular vesicle isolation, analysis and therapy in cancer

et al. 2022

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“…In the following study, the authors developed a vacuum-driven power-free microfluidic chip for exosomal mRNA detection [ 91 ]. The chip was fabricated in a “Y” shape with a channel height of 0.1 mm and width of 0.6 mm.…”

Section: Passive (Non-mechanical Systems)mentioning

confidence: 99%

Novel Pumping Methods for Microfluidic Devices: A Comprehensive Review

2022

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This review is an account of methods that use various strategies to control microfluidic flow control with high accuracy. The reviewed systems are divided into two large groups based on the way they create flow: passive systems (non-mechanical systems) and active (mechanical) systems. Each group is presented by a number of device fabrications. We try to explain the main principles of operation, and we list advantages and disadvantages of the presented systems. Mechanical systems are considered in more detail, as they are currently an area of increased interest due to their unique precision flow control and “multitasking”. These systems are often applied as mini-laboratories, working autonomously without any additional operations, provided by humans, which is very important under complicated conditions. We also reviewed the integration of autonomous microfluidic systems with a smartphone or single-board computer when all data are retrieved and processed without using a personal computer. In addition, we discuss future trends and possible solutions for further development of this area of technology.

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Microfluidic device for one-step detection of breast cancer-derived exosomal mRNA in blood using signal-amplifiable 3D nanostructure

Cited by 40 publications

References 52 publications

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Microfluidic Point-of-Care (POC) Devices in Early Diagnosis: A Review of Opportunities and Challenges

Microfluidic platforms for extracellular vesicle isolation, analysis and therapy in cancer

Novel Pumping Methods for Microfluidic Devices: A Comprehensive Review

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