Microfluidic Device for Cell Trapping with Carbon Electrodes Using Dielectrophoresis

Puri, Paridhi; Kumar, Vijay; Belgamwar, Sachin U.; Sharma, Niti Nipun

doi:10.1007/s10544-018-0350-0

Cited by 22 publications

(21 citation statements)

References 37 publications

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“…Since the first report of the microfabrication technique for DEP devices in 1988, the 1990s saw an explosion of implementing DEP experiments with metal microelectrodes. The microelectrodes commonly used are carbon and metals, such as gold , platinum , titanium or silicon , among which gold and platinum electrodes are ideal choices due to the stable electrochemical property.…”

Section: Configurations Of Electrodes and Dep Devicesmentioning

confidence: 99%

“…The reduced material cost and simplicity make carbon electrodes attractive in 3D electrode fabrication . More recently, a DEP device with C‐serpentine 3D carbon electrodes was demonstrated to be capable of separating living/dead yeast cells . The 3D carbon electrodes were fabricated simply by bonding the microchannel with 3D carbon electrodes and glass.…”

Section: Cell Viability Assessmentmentioning

confidence: 99%

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Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

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Recent advances in dielectrophoresis-based cell viability assessmentDielectrophoresis (DEP) is a non-destructive, accurate, and label-free cell manipulating technique and DEP applications have been found in various fields. Assessment of cell viability is one of the important applications and many investigations have been reported. In this paper, cell polarization and its modeling, some key parameters employed for living/dead cell separation, as well as electrode configurations are reviewed. Focus is given to the latest development of DEP devices employed for the assessment of cell viability. Experimentally determined factors for separating living/dead cells, such as the conductivity of suspending medium and the frequency of applied electric field, are summarized. The future directions and potential challenges in this field are also outlined. Theory of cell DEPThe fundamentals of DEP were systematically reviewed in the past [35,36]. In this section, we only focus on the theory concerning cellular DEP. Based on the spherical particle model, single-shell model and multi-shell model of biological cells are extended. Color online: See the article online to view Figs. 1-7 in color. J. Zhang et al. Electrophoresis 2020, 41, 917-932

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Section: Configurations Of Electrodes and Dep Devicesmentioning

confidence: 99%

Section: Cell Viability Assessmentmentioning

confidence: 99%

Recent advances in dielectrophoresis‐based cell viability assessment

et al. 2020

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“…( E) Serpentine‐shaped carbon electrode used to separate live from dead yeast cells. Reprinted with permission from [59] copyright (2018) Springer Science+Business Media, LLC, part of Springer Nature. ( F) Array of optically induced (or virtual) electrodes, projected on a photoconductive layer over an ITO surface used to manipulated the trajectory of a cancer cell cluster.…”

Section: Voltage Requirements In Edep Devicesmentioning

confidence: 99%

Toward low‐voltage dielectrophoresis‐based microfluidic systems: A review

2020

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Dielectrophoretically driven microfluidic devices have demonstrated great applicability in biomedical engineering, diagnostic medicine, and biological research. One of the potential fields of application for this technology is in point‐of‐care (POC) devices, ideally allowing for portable, fully integrated, easy to use, low‐cost diagnostic platforms. Two main approaches exist to induce dielectrophoresis (DEP) on suspended particles, that is, electrode‐based DEP and insulator‐based DEP, each featuring different advantages and disadvantages. However, a shared concern lies in the input voltage used to generate the electric field necessary for DEP to take place. Therefore, input voltage can determine portability of a microfluidic device. This review outlines the recent advances in reducing stimulation voltage requirements in DEP‐driven microfluidics.

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“…Owing to their controllability over artificial gravity magnitude, numerous centrifugal microfluidic platforms have been proposed 195 by using rotating disks. Most of them are relevant to blood sample preparation, 196 cell-based assays, 197 and DNA extraction 198 . On the other hand, natural gravity-driven microfluidic systems can also deliver high separation efficiency with specified channel designs.…”

Section: Gravity-based Sortingmentioning

confidence: 99%

Label-free microfluidic sorting of microparticles

et al. 2019

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Massive growth of the microfluidics field has triggered numerous advances in focusing, separating, ordering, concentrating, and mixing of microparticles. Microfluidic systems capable of performing these functions are rapidly finding applications in industrial, environmental, and biomedical fields. Passive and label-free methods are one of the major categories of such systems that have received enormous attention owing to device operational simplicity and low costs. With new platforms continuously being proposed, our aim here is to provide an updated overview of the state of the art for passive label-free microparticle separation, with emphasis on performance and operational conditions. In addition to the now common separation approaches using Newtonian flows, such as deterministic lateral displacement, pinched flow fractionation, cross-flow filtration, hydrodynamic filtration, and inertial microfluidics, we also discuss separation approaches using non-Newtonian, viscoelastic flow. We then highlight the newly emerging approach based on shear-induced diffusion, which enables direct processing of complex samples such as untreated whole blood. Finally, we hope that an improved understanding of label-free passive sorting approaches can lead to sophisticated and useful platforms toward automation in industrial, environmental, and biomedical fields.

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Microfluidic Device for Cell Trapping with Carbon Electrodes Using Dielectrophoresis

Cited by 22 publications

References 37 publications

Recent advances in dielectrophoresis‐based cell viability assessment

Recent advances in dielectrophoresis‐based cell viability assessment

Toward low‐voltage dielectrophoresis‐based microfluidic systems: A review

Label-free microfluidic sorting of microparticles

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