Microfluidic-based biosensor: signal enhancement by gold nanoparticle

Lakshmipriya, Thangavel; Hashim, U.; Gopinath, Subash C. B.; Azizah, N.

doi:10.1007/s00542-016-3074-1

Cited by 14 publications

(6 citation statements)

References 46 publications

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“…Interaction of nanomaterials with biological entities, such as proteins, enzymes, DNA and RNA oligonucleotides has emerged as an interdisciplinary eld known as "nanobiotechnology", which refers to the methodological approaches that nanoparticles or nanomaterials are combined to create tools for investigation of biological systems. 1 Integration of nanomaterials into the sensing systems as the active elements (transducer or detector) has paved the way for a signicant breakthrough in the eld, resulting in stable sensing probes, 2,3 enhanced detection signals in small sample volumes, 4 miniaturised tools 5 and systems for multiplex detection. 6 The discovery of nanoparticles and following development of new instruments for nanoparticle characterisation between 1990 and 2000 has facilitated the fabrication of smart biosensing tools in the early 2000s.…”

Section: Introductionmentioning

confidence: 99%

How to make nanobiosensors: surface modification and characterisation of nanomaterials for biosensing applications

2017

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This report aims to provide the audience with a guideline for construction and characterisation of nanobiosensors that are based on widely used affinity probes including antibodies and aptamers and nanomaterials such as carbon-based nanomaterials, plasmonic nanomaterials and luminescent nanomaterials. The affinity probes and major methodologies that have been extensively used to make nanobiosensors, such as thiol-metal interactions, avidin-biotin interaction, p-interactions and EDC-NHS chemistry, were described with the most recent examples from the literature. Characterisation techniques that have been practised to validate nanoparticle surface modification with antibodies and aptamers, including gel electrophoresis, ultraviolet-visible spectrophotometry, dynamic light scattering and circular dichroism were described with examples. This report mainly covers the reports published between

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Section: Introductionmentioning

confidence: 99%

How to make nanobiosensors: surface modification and characterisation of nanomaterials for biosensing applications

2017

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“…Despite their size, nanomaterial can change color in response to variations in their forms, such as bulk, sheets, or in cylinders. Nanomaterial has been widely applied for various biotechnological and biomedical purposes . Recently, carbon nanotubes have received a great deal of attention.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of functionalized single‐walled carbon nanotube with herbal extract of Hempedu bumi

Foo

Anbu

Gopinath

et al. 2018

Surface & Interface Analysis

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The primary purpose of this study was to determine the antimicrobial activity of functionalized single-walled carbon nanotube (SWNT) using an extract of the herb, Hempedu bumi. H bumi extract and H bumi extract complexed SWNT were evaluated for biological activities against Bacillus sp., (pathogen) Escherichia coli (opportunistic pathogen), and Aspergillus niger (pathogen).The formation of inhibition zones of these 3 microbes was measured to be evident for the functionalized SWNT with H bumi. Further, morphological and structural analyses were conducted to investigate the functionalized SWNT with H bumi using scanning electron microscopy, atomic force microscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy, well supporting the intact and crystalline nature of the SWNT. Fourier transform infrared spectroscopy result shows the highest peak at 3371.48 cm −1 , representing an andrographolide group from the plant extract. An apparent clear zone has been noticed with SWNT conjugated H bumi extract, displaying a zone of inhibition larger than 1.0 cm against the tested microbes. The results indicate that SWNT has the potential for use as a carrier of components from plant extracts.

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“…In this process, the carboxylic encapsulated gold nanoparticles having 10 nm diameter were added on top of the SAM-coated cantilevers and were incubated for 10–12 h. The purpose of this step was to enhance the sensitivity of the sensing platform. The nanoparticles not only provide a better electron transfer rate and enhanced orientational freedom, but also a high surface-to-volume ratio that enables the feature of immobilizing more functionalized antibodies and subsequently more target antigens/antibodies based on the user's final target analyte [ [24] , [25] , [26] ]. This phenomenon results in a substantial increment in the probability of capturing the ‘capture antibodies’ even at very low concentrations that contribute to better sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Piezoelectric point-of-care biosensor for the detection of SARS-COV-2 (COVID-19) antibodies

Mandal

Indaleeb

Younan

et al. 2022

Sensing and Bio-Sensing Research

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Microfluidic-based biosensor: signal enhancement by gold nanoparticle

Cited by 14 publications

References 46 publications

How to make nanobiosensors: surface modification and characterisation of nanomaterials for biosensing applications

How to make nanobiosensors: surface modification and characterisation of nanomaterials for biosensing applications

Antimicrobial activity of functionalized single‐walled carbon nanotube with herbal extract of Hempedu bumi

Piezoelectric point-of-care biosensor for the detection of SARS-COV-2 (COVID-19) antibodies

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