Microfluidic aptasensor POC device for determination of whole blood potassium

Tseng, Chin-Chung; Lu, Song-Yu; Chen, Szu-Jui; Wang, Ju‐Ming; Fu, Lung-Ming; Wu, Yihong

doi:10.1016/j.aca.2022.339722

Cited by 21 publications

(5 citation statements)

References 65 publications

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“…range from 0.05 × 10 −3 mol/L to 9 × 10 −3 mol/L and have a limit of detection (LOD) of 0.01 × 10 −3 mol/L. This device demonstrates that low-cost POC microfluidic sensors with on-chip blood separations (serum from whole blood) are attainable [27]. Another example of the capability of these devices is in the separation of extracellular vesicles such as exosomes.…”

Section: Introductionmentioning

confidence: 92%

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Torres-Castro,

Acuña-Umaña,

Lesser-Rojas

et al. 2023

Micromachines

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Blood is a complex sample comprised mostly of plasma, red blood cells (RBCs), and other cells whose concentrations correlate to physiological or pathological health conditions. There are also many blood-circulating biomarkers, such as circulating tumor cells (CTCs) and various pathogens, that can be used as measurands to diagnose certain diseases. Microfluidic devices are attractive analytical tools for separating blood components in point-of-care (POC) applications. These platforms have the potential advantage of, among other features, being compact and portable. These features can eventually be exploited in clinics and rapid tests performed in households and low-income scenarios. Microfluidic systems have the added benefit of only needing small volumes of blood drawn from patients (from nanoliters to milliliters) while integrating (within the devices) the steps required before detecting analytes. Hence, these systems will reduce the associated costs of purifying blood components of interest (e.g., specific groups of cells or blood biomarkers) for studying and quantifying collected blood fractions. The microfluidic blood separation field has grown since the 2000s, and important advances have been reported in the last few years. Nonetheless, real POC microfluidic blood separation platforms are still elusive. A widespread consensus on what key figures of merit should be reported to assess the quality and yield of these platforms has not been achieved. Knowing what parameters should be reported for microfluidic blood separations will help achieve that consensus and establish a clear road map to promote further commercialization of these devices and attain real POC applications. This review provides an overview of the separation techniques currently used to separate blood components for higher throughput separations (number of cells or particles per minute). We present a summary of the critical parameters that should be considered when designing such devices and the figures of merit that should be explicitly reported when presenting a device’s separation capabilities. Ultimately, reporting the relevant figures of merit will benefit this growing community and help pave the road toward commercialization of these microfluidic systems.

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Section: Introductionmentioning

confidence: 92%

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Torres-Castro,

Acuña-Umaña,

Lesser-Rojas

et al. 2023

Micromachines

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“…The integration of microfluidic platforms with disposable paper strips has also been utilized for aptamer-based biomarker detection applications [115][116][117][118][119][120][121]. Recently, a low-cost and smartphone-adapted sensing platform was introduced for measuring potassium ion (K + ) levels in whole blood, which is an essential parameter for chronic kidney disease [58]. In short, the platform comprises PMMA-sealed paper microfluidic chip and finger pumps for transporting different reagents into the reaction chamber.…”

Section: Paper-based Colorimetric Aptasensorsmentioning

confidence: 99%

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods

et al. 2023

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Recent innovations in point-of-care (POC) diagnostic technologies have paved a critical road for the improved application of biomedicine through the deployment of accurate and affordable programs into resource-scarce settings. The utilization of antibodies as a bio-recognition element in POC devices is currently limited due to obstacles associated with cost and production, impeding its widespread adoption. One promising alternative, on the other hand, is aptamer integration, i.e., short sequences of single-stranded DNA and RNA structures. The advantageous properties of these molecules are as follows: small molecular size, amenability to chemical modification, low- or nonimmunogenic characteristics, and their reproducibility within a short generation time. The utilization of these aforementioned features is critical in developing sensitive and portable POC systems. Furthermore, the deficiencies related to past experimental efforts to improve biosensor schematics, including the design of biorecognition elements, can be tackled with the integration of computational tools. These complementary tools enable the prediction of the reliability and functionality of the molecular structure of aptamers. In this review, we have overviewed the usage of aptamers in the development of novel and portable POC devices, in addition to highlighting the insights that simulations and other computational methods can provide into the use of aptamer modeling for POC integration.

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“…The demand for microflow devices dedicated to automatic detection and quantification of chosen ions is undeniable, and the best example of such is the one developed by Chin-Chung Tseng et al [ 121 ]. This is a typical Point-of-Care device.…”

Section: Microfluidic Aptamer-based Sensors For Ions Detectionmentioning

confidence: 99%

“… ( a ) The example of aptamer biochip to K + detection and the dedicated device for operation, ( b ) Reaction color for different volumes of AuNPs and concentrations of 3 mLNaCl together with reaction color for 8 mL AuNPs, 3 mL NaCl (0.4 M) and different volumes of aptamers [ 121 ]. …”

Section: Figurementioning

confidence: 99%

Recent Achievements in Electrochemical and Optical Nucleic Acids Based Detection of Metal Ions

Jarczewska

Szymczyk²,

Zajda³

et al. 2022

Molecules

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Recently nucleic acids gained considerable attention as selective receptors of metal ions. This is because of the possibility of adjusting their sequences in new aptamers selection, as well as the convenience of elaborating new detection mechanisms. Such a flexibility allows for easy utilization of newly emerging nanomaterials for the development of detection devices. This, in turn, can significantly increase, e.g., analytical signal intensity, both optical and electrochemical, and the same can allow for obtaining exceptionally low detection limits and fast biosensor responses. All these properties, together with low power consumption, make nucleic acids biosensors perfect candidates as detection elements of fully automatic portable microfluidic devices. This review provides current progress in nucleic acids application in monitoring environmentally and clinically important metal ions in the electrochemical or optical manner. In addition, several examples of such biosensor applications in portable microfluidic devices are shown.

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Microfluidic aptasensor POC device for determination of whole blood potassium

Cited by 21 publications

References 65 publications

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Aptamer-Based Point-of-Care Devices: Emerging Technologies and Integration of Computational Methods

Recent Achievements in Electrochemical and Optical Nucleic Acids Based Detection of Metal Ions

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