Microflow1, a sheathless fiber‐optic flow cytometry biomedical platform: Demonstration onboard the international space station

Dubeau-Laramée, Geneviève; Rivière, Christophe; Jean, Isabelle; Mermut, Ozzy; Cohen, Luchino Y.

doi:10.1002/cyto.a.22427

Cited by 21 publications

(13 citation statements)

References 27 publications

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“…Another device that has been tested in parabolic flight and aboard the ISS is the fiber optic-based flow cytometer Microflow1 (Cohen et al, 2008, Dubeau-Laramée et al, 2014). Cells flow in a capillary tube perpendicular to a 488 nm laser diode excitation source.…”

Section: Space Travelmentioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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Point-of-care or point-of-use diagnostics are analytical devices that provide clinically relevant information without the need for a core clinical laboratory. In this review we define point-of-care diagnostics as portable versions of assays performed in a traditional clinical chemistry laboratory. This review discusses five areas relevant to human and animal health where increased attention could produce significant impact: veterinary medicine, space travel, sports medicine, emergency medicine, and operating room efficiency. For each of these areas, clinical need, available commercial products, and ongoing research into new devices are highlighted.

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Section: Space Travelmentioning

confidence: 99%

Point-of-care diagnostics for niche applications

Cummins

Ligler

Walker

2016

Biotechnology Advances

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“…Owing to the high versatility for diagnostic medicine (e.g., hematology, immunology, and physiology), this technology was so far retained as a prime asset for health monitoring and clinical laboratory diagnostics for astronauts, in view of the upcoming deep-space exploration missions (Crucian and Sams, 2012). A flow cytometry platform was already successfully tested on-board the ISS to monitor and understand the physiological adaptations of astronauts to microgravity (Dubeau-Laramée et al, 2014;Phipps et al, 2014) (Figure 3). Advanced developments of the prototype were based on commercialized flow cytometer with significant additional engineering modifications, mostly aimed to generate laminar particle flow (distinct from the standard sheath fluid based method), and to reduce the significant amount of liquid biohazardous waste and energy operating requirements.…”

Section: Flow Cytometrymentioning

confidence: 99%

Water and Microbial Monitoring Technologies towards the Near Future Space Exploration

Amalfitano¹,

Levantesi²,

Copetti³

et al. 2019

Preprint

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Space exploration is demanding longer lasting human missions and water resupply from Earth will become increasingly unrealistic. In a near future, the spacecraft water monitoring systems will require technological advances to promptly identify and counteract contingent events of waterborne microbial contamination, posing health risks to astronauts with lowered immune responsiveness. The search for bio-analytical approaches, alternative to those applied on Earth by cultivation-dependent methods, is pushed by the compelling need to limit waste disposal and avoid microbial regrowth from analytical carryovers. Prospective technologies will be selected only if first validated in a flight-like environment, by following basic principles, advantages, and limitations beyond their current applications on Earth. Starting from the water monitoring activities applied on the International Space Station, we provide a critical overview of the nucleic acid amplification-based approaches (i.e., loop-mediated isothermal amplification, quantitative PCR, and high-throughput sequencing) and early-warning methods for total microbial load assessments (i.e., ATP-metry, flow cytometry), already used at a high readiness level aboard crewed space vehicles. Our findings suggest that the forthcoming space applications of mature technologies will be necessarily bounded by a compromise between analytical performances (e.g., speed to results, identification depth, reproducibility, multiparametricity) and detrimental technical requirements (e.g., reagent usage, waste production, operator skills, crew time). As space exploration progresses toward extended missions to Moon and Mars, miniaturized systems that also minimize crew involvement in their end-to-end operation are likely applicable on the long-term and suitable for the in-flight water and microbiological research.

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“…2,3 With continuing development of modern technologies such as the CSA's miniaturised, low-cost, and portable fl ow cytometer for ISS orbital operations, the ability to do more complex and complete blood analyses will be available in situ. 8 Space assets are readily available and being used to benefi t global health, including Ebola virus disease and other infectious diseases. This benefi t could be further enhanced by greater cooperation, investment, and partnership between the space sector and public health and humanitarian organisations.…”

Section: Help From Above: Outer Space and The Fi Ght Against Ebolamentioning

confidence: 99%