We demonstrate the celluloid-based biochip for cell trapping, prepared by Suzuki's universal microprinting (SUMP) method. The structure of 8 × 8 µm 2 micoenclosures consists of a micropillar array with 2 µm spaces between 2-µm-diameter pillars. We succeeded in fabricating the single-cell trap for yeast cells by using the celluloid microenclosure array chip.In addition, we demonstrated the fabrication process of the celluloid microlens array by the SUMP method and XeF 2 vapor etching. By using this celluloid microlens array, we carried out experiments on collecting laser light and observed the focal spots for each microlens. We consider that this simple technique will be very useful for the single-cell isolation and analysis of microbial cells.