Microfabrication meets microbiology

Weibel, Douglas B.; DiLuzio, Willow R.; Whitesides, George M.

doi:10.1038/nrmicro1616

Cited by 709 publications

(649 citation statements)

References 73 publications

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“…The device was fabricated using soft lithography techniques described previously (Weibel et al, 2007;Seymour et al, 2008), bonded to a microscope slide, and mounted onto a Nikon Ti microscope (Tokyo, Japan). All experiments were conducted at the same temperature at which the cells were grown, using a temperature controlled stage insert (Warner Instruments, Hamden, CT, USA).…”

Section: Microfluidic Chemotaxis Experimentsmentioning

confidence: 99%

Temperature-induced behavioral switches in a bacterial coral pathogen

et al. 2015

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Evidence to date indicates that elevated seawater temperatures increase the occurrence of coral disease, which is frequently microbial in origin. Microbial behaviors such as motility and chemotaxis are often implicated in coral colonization and infection, yet little is known about the effect of warming temperatures on these behaviors. Here we present data demonstrating that increasing water temperatures induce two behavioral switches in the coral pathogen Vibrio coralliilyticus that considerably augment the bacterium's performance in tracking the chemical signals of its coral host, Pocillopora damicornis. Coupling field-based heat-stress manipulations with laboratory-based observations in microfluidic devices, we recorded the swimming behavior of thousands of individual pathogen cells at different temperatures, associated with current and future climate scenarios. When temperature reached ⩾ 23°C, we found that the pathogen's chemotactic ability toward coral mucus increased by 460%, denoting an enhanced capability to track host-derived chemical cues. Raising the temperature further, to 30°C, increased the pathogen's chemokinetic ability by 457%, denoting an enhanced capability of cells to accelerate in favorable, mucus-rich chemical conditions. This work demonstrates that increasing temperature can have strong, multifarious effects that enhance the motile behaviors and host-seeking efficiency of a marine bacterial pathogen.

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Section: Microfluidic Chemotaxis Experimentsmentioning

confidence: 99%

Temperature-induced behavioral switches in a bacterial coral pathogen

et al. 2015

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“…53 For PDMS, O 2 plasma treatment needs to be performed immediately before aligning the membranes and pressing them together. 36,54 30 …”

Section: Tpe Membranes With Open Through-holesmentioning

confidence: 99%

“…However, almost all designs of microfluidic patterning devices have relied on PDMS, 18,19,[31][32][33][34][35][36][37][40][41][42][43][44][45][46][47][48][49] which is not very amendable to low cost massproduction. [50][51][52][53] PDMS is also an hydrophobic material, 50 30 which favors the trapping of air bubbles in the channels and prevents the use of capillary action to fill the devices. Although the surface of PDMS can be made temporarily hydrophilic using O 2 plasma treatment, it will recover its hydrophobic state within few hours, which is problematic for 35 3D devices assembled from multiple layers.…”

Section: Introductionmentioning

confidence: 99%

3D thermoplastic elastomer microfluidic devices for biological probe immobilization

Brassard

Clime

et al. 2011

Lab Chip

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Microfluidics has emerged as a valuable tool for the high-resolution patterning of biological probes on solid supports. Yet, its widespread adoption as a universal biological immobilization tool is still limited by several technical challenges, particularly for the patterning of isolated spots using three-dimensional (3D) channel networks. A key limitation arises from the difficulties to adapt the techniques and materials typically used in prototyping to low-cost mass-production. In this paper, we present the fabrication of thin thermoplastic elastomer membranes with microscopic through-holes using a hotembossing process that is compatible with high-throughput manufacturing. The membranes provide the basis for the fabrication of highly integrated 3D microfluidic devices with a footprint of only 1 Â 1 cm 2 . When placed on a solid support, the device allows for the immobilization of up to 96 different probes in the form of a 10 Â 10 array comprising isolated spots of 50 Â 50 mm 2 . The design of the channel network is optimized using 3D simulations based on the Lattice-Boltzmann method to promote capillary action as the sole force distributing the liquid in the device. Finally, we demonstrate the patterning of DNA and protein arrays on hard thermoplastic substrates yielding spots of excellent definition that prove to be highly specific in subsequent hybridization experiments.

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“…Miniaturization has become a important factor in all spheres of modern society, reflected strongly in science and technology, including electronics [1], medicine [2], chemistry [3,4] and other areas [5,6]. Miniaturization in chemistry was given a new dimension in the 1990s with the concepts of lab-on-a-chip and micro-total analysis systems ([ TAS) [7][8][9][10][11].…”

Section: Trends In Miniaturizationmentioning

confidence: 99%

Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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Miniaturized, portable instrumentation has been gaining popularity in all areas of analytical chemistry. Capillary electrophoresis (CE), due to its main strengths of high separation efficiency, relatively short analysis time and low consumption of chemicals, is a particularly suitable technique for use in portable analytical instrumentation. In line with the general trend in miniaturization in chemistry utilizing microfluidic chips, the main thrust of portable CE (P-CE) systems development is towards chip-based miniaturized CE. Despite this, capillary-based (non-chip) P-CE systems have certain unmatched advantages, especially in the relative simplicity of the regular cylindrical geometry of the CE capillary, maximal volume-to-surface ratio, no need to design and to fabricate a chip, the low costs of capillary compared to chip, and better performance with some detection techniques. This review presents an overview of the state of the art of P-CE and literature relevant to future developments. We pay particular attention to the development and the potential of miniaturization of functional parts for P-CE. These include components related to sample introduction, separation and detection, which are the key elements in P-CE design. The future of P-CE may be in relatively simple, rugged designs (e.g., using a short piece of capillary fixed to a chip-sized platform on which injection and detection parts can be mounted). Electrochemical detection is well suited for miniaturization, so is probably the most suitable detection technique for P-CE, but optical detection is gaining interest, especially due to miniaturized light sources (e.g., light-emitting diodes).

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Microfabrication meets microbiology

Cited by 709 publications

References 73 publications

Temperature-induced behavioral switches in a bacterial coral pathogen

Temperature-induced behavioral switches in a bacterial coral pathogen

3D thermoplastic elastomer microfluidic devices for biological probe immobilization

Portable capillary-based (non-chip) capillary electrophoresis

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