Microextraction by packed sorbent followed by ultra high performance liquid chromatography for the fast extraction and determination of six antidepressants in urine

Fuentes, A.M. Ares; Fernández, P.; Fernández, Ana; Carro, A.M.; Lorenzo, R.A.

doi:10.1002/jssc.201900060

Cited by 15 publications

(8 citation statements)

References 41 publications

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“…One of the steps to take into account in an analytical method is the isolation and concentration of analytes of interest from the biological samples under study; the most used procedures for extracting antidepressants are liquid-liquid extraction (LLE) [6,17], solid-phase extraction (SPE) [6,[18][19][20], and some miniaturized techniques-such as solidphase microextraction (SPME) [6,21,22], microextraction by packed sorbent (MEPS) [6,[23][24][25], and dispersive liquid-liquid microextraction (DLLME) [6,[26][27][28][29][30][31]. There are also several methods involving gas chromatography (GC) and liquid chromatography (LC) coupled with mass spectrometry (MS) [26,[30][31][32][33][34][35][36][37][38] or tandem mass spectrometry (MS/MS) [27,[39][40][41][42][43], ultraviolet (UV) [22,28,[44][45][46][47][48], fluorescence [25], diode array (DAD) or photodiode array (PDA)…”

Section: Introductionmentioning

confidence: 99%

New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling

et al. 2021

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The increase in the consumption of antidepressants is a public health problem worldwide, as these are a class of compounds widely used in the treatment of several illnesses, such as depression and anxiety. This work aimed to develop and optimize a method for the quantification of a number of antidepressants and their metabolites (fluoxetine, venlafaxine, O-desmethylvenlafaxine, citalopram, sertraline, and paroxetine) in 100 µL of oral fluid using the dried saliva spots (DSS) sampling approach and gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The method was validated, presenting linearity within the studied range, with detection and quantification limits ranging between 10 and 100 ng/mL, and coefficients of determination (R2) of at least 0.99 for all analytes. Recoveries were between approximately 13 and 46%. The analysis of precision and accuracy presented acceptable coefficients of variation and relative errors, considering the criteria usually accepted in the validation of bioanalytical procedures. The method herein described is the first to be reported using DSS for the extraction of antidepressants, proving to be a sensitive, simple, and fast alternative to conventional techniques, and capable of being routinely applied in clinical and forensic toxicology scenarios.

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Section: Introductionmentioning

confidence: 99%

New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling

et al. 2021

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“…One year later Klimowska and Wielgomas (2018) In this work the range of linearity was 25-1,000 ng/ml urine (Fuentes et al, 2019). Biagini et al ( 2020) described a reliable analytical procedure combining MEPS with UHPLC-electrospray ionization tandem MS for determination of 8-iso-prostaglandin F2α, 8-iso-prostaglandin E2 and prostaglandin E2 in dried blood spots (Biagini et al, 2020).…”

Section: Meps Applicationsmentioning

confidence: 75%

“…The optimized metal-organic framework (MOF)-MEPS method offered an acceptable efficiency for the recovery of MA from urine samples (MOF-5@Fe 3 O 4 -NH 2 and MOF-5@SBA-15: 94.5 and 90.3%, respectively, RSD <3.54%). The limit of detection (LOD) of MA calculated by the MOF-MEPS procedure combined with HPLC for MOF-5@Fe 3 O 4 -NH 2 and MOF-5@SBA-15 were calculated to be 0.10 and 0.13 μg/ml, respectively(Rahimpoor et al, 2019).At the same timeFuentes et al (2019) developed MEPS coupled with UHPLC with photodiode array detection for analysis of the antidepressants mirtazapine, venlafaxine, escitalopram, fluoxetine, fluvoxamine and sertraline in human urine samples.…”

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confidence: 99%

Recent applications of microextraction sample preparation techniques in biological samples analysis

Daryanavard

Zolfaghari

Abdel-Rehim

et al. 2021

Biomedical Chromatography

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Analysis of biological samples is affected by interfering substances with chemical properties similar to those of the target analytes, such as drugs. Biological samples such as whole blood, plasma, serum, urine and saliva must be properly processed for separation, purification, enrichment and chemical modification to meet the requirements of the analytical instruments. This causes the sample preparation stage to be of undeniable importance in the analysis of such samples through methods such as microextraction techniques. The scope of this review will cover a comprehensive summary of available literature data on microextraction techniques playing a key role for analytical purposes, methods of their implementation in common biological samples, and finally, the most recent examples of application of microextraction techniques in preconcentration of analytes from urine, blood and saliva samples. The objectives and merits of each microextration technique are carefully described in detail with respect to the nature of the biological samples. This review presents the most recent and innovative work published on microextraction application in common biological samples, mostly focused on original studies reported from 2017 to date. The main sections of this review comprise an introduction to the microextraction techniques supported by recent application studies involving quantitative and qualitative results and summaries of the most significant, recently published applications of microextracion methods in biological samples. This article considers recent applications of several microextraction techniques in the field of sample preparation for biological samples including urine, blood and saliva, with consideration for extraction techniques, sample preparation and instrumental detection systems.

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“…First, as our paper presents the use of a synthesized graphene-based sorbent packed into a capillary extraction column, its robustness is noteworthy, as just described, given that it was applied to more than 250 injections. As examples, other recent works pinpoint their lab-made extractive hardware being re-used five and seventy times without losing its efficiency, respectively [20,21]. Likewise, our developed extraction column surpasses by far the commercially available SPE cartridges, which can be ideally used only once.…”

Section: Overall Methods Performancementioning

confidence: 84%

Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine

et al. 2020

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Human mental disorders can be currently classified as one of the most relevant health topics. Including in this are depression and anxiety, which can affect us at any stage of life, causing economic and social problems. The treatments involve cognitive psychotherapy, and mainly the oral intake of pharmaceutical antidepressants. Therefore, the development of analytical methods for monitoring the levels of these drugs in biological fluids is critical. Considering the current demand for sensitive and automated analytical methods, the coupling between liquid chromatography and mass spectrometry, combined with suitable sample preparation, becomes a useful way to improve the analytical results even more. Herein we present an automated multidimensional method based on high-performance liquid chromatography-tandem mass spectrometry using a lab-made, graphene-based capillary extraction column connected to a C8 analytical column to determined five pharmaceutical drugs in urine. A method enhancement was performed by considering the chromatographic separation and the variables of the loading phase, loading time, loading flow, and injection volume. Under optimized conditions, the study reports good linearity with R2 > 0.98, and limits of detection in the range of 0.5–20 µg L−1. Afterward, the method was applied to the direct analysis of ten untreated urine samples, reporting traces of citalopram in one of them. The results suggest that the proposed approach could be a promising alternative that provides direct and fully automated analysis of pharmaceutical drugs in complex biological matrices.

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Microextraction by packed sorbent followed by ultra high performance liquid chromatography for the fast extraction and determination of six antidepressants in urine

Cited by 15 publications

References 41 publications

New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling

New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling

Recent applications of microextraction sample preparation techniques in biological samples analysis

Multidimensional Liquid Chromatography Employing a Graphene Oxide Capillary Column as the First Dimension: Determination of Antidepressant and Antiepileptic Drugs in Urine

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