Microemulsion formulation of a new biopesticide to control the diamondback moth (Lepidoptera: Plutellidae)

Abstract

This study was designed to develop a microemulsion formulation of norcantharidin for the control of the diamondback moth (DBM), Plutella xylostella (Linnaeus), a notorious pest of brassica crops worldwide. The oil phase was screened and selected based on norcantharidin solubility while the surfactants were selected on the basis of their efficiency to form microemulsion. Optimized batches were selected using pseudo ternary phase diagrams. The microemulsion system were stabilized using mixtures composed of norcantharidin, surfactants (Tx13 and Tw80), and cosurfactant (ethanol). Its physicochemical characteristics were also demonstrated to have a higher cloud point than 72 °C as well as good thermodynamic and dilution stability. In additon, a subsequent insecticidal bioassay indicated that the acute LC50 for norcantharidin microemulsion to P. xylostella was estimated to be 12.477 mg/L (11.58–13.41, 95% CL). Our results provide an environment-friendly promising alternative to control P. xylostella and possibly contribute to ameliorating any pesticide resistance in P. xylostella.

OIL-IN-WATER (O/W) EMULSIONABLE CONCENTRATE OF ISHPINK (Ocotea quixos) WITH THERMODYNAMIC STABILITY

“…All emulsions exhibited excellent stability after being subjected to the aging treatment, maintaining the yellow coloration and translucent shade; likewise, no phase separation was observed at both room temperature and after the aging treatment. These results were similar to those reported by Shao et al (2018) when evaluating the stability of a microemulsion with the active ingredient norcantharidin for controlling P. xylostella.…”

Synthesis and Technology of Nanoemulsion-Based Pesticide Formulation

“…Bifenthrin nanoemulsions have also increased the mortality rate of cabbage maggots to 100% after 20 h of exposure time [139]. A microemulsion based on norcantharidin [140] and emamectin benzoate [19] has been formulated to control diamondback moth. Other nanoemulsions have been developed to containing organophosphorus and pyrethroid insecticides, and they also have shown a better effect than their counterparts, free insecticides [97,141].…”

WITHDRAWN: Bioengineering of Neem Colloidal Nano-Emulsion Formulation With Adjuvant for Better Surface Adhesion and Long Term Activity in Insect Control

“…Occurrence of Ostwald ripening was avoided by increasing the elasticity of droplet 103 and the addition of natural adjuvant which reduced interfacial free energy forming a mechanical barrier against coalescence. Prosopis juli ora, may be acted as bio-polymeric adjuvant due to presence of cellulose ber 28,29 in this formulation.…”

“…Polydispersity values below 0.2 indicate uniformity among oil droplet sizes or monomodal distributions and therefore better stability, whereas values close to 1 indicate a heterogeneous or multimodal distribution 29 . In the present study, values were closer to 0.3 indicating mono-dispersive nature of developed nano-emulsion with good stability during storage.…”