Microelectrodes for studying neurobiology

Kita, Justin M.; Wightman, R. Mark

doi:10.1016/j.cbpa.2008.06.035

Cited by 45 publications

(35 citation statements)

References 44 publications

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“…As h → ∞, the capped cylinder approaches an infinite cylinder, which we will simply refer to as a cylinder in the following. The extensive series of articles of the Wightman and coworkers [10][11][12][13][14][15] (to cite just a few) on the use of cylindrical electrodes in neuroscience attest to the application of these electrodes in practice. McNally et al [16] developed a conical-tip electrode (recently simulated [17]) which they considered as close to cylindrical and applied an approximate analytical solution due to Amatore [18].…”

Section: Introductionmentioning

confidence: 99%

Reference values of the chronoamperometric response at cylindrical and capped cylindrical electrodes

Britz¹,

Østerby²,

Strutwolf³

2010

Electrochimica Acta

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Section: Introductionmentioning

confidence: 99%

Reference values of the chronoamperometric response at cylindrical and capped cylindrical electrodes

Britz¹,

Østerby²,

Strutwolf³

2010

Electrochimica Acta

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“…(8) Single cell electrochemical measurements correlate well with both single vesicle measurements and bulk assays. (9, 10) In this case, CFMA can be used to measure from single mast cells (11) based on the high spatial resolution of these electrodes and the high sensitivity and low background current achieved.…”

mentioning

confidence: 78%

Carbon-Fiber Microelectrode Amperometry Reveals Sickle-Cell-Induced Inflammation and Chronic Morphine Effects on Single Mast Cells

et al. 2012

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Sickle cell disease, caused by a mutation of hemoglobin, is characterized by a complex pathophysiology including an important inflammatory component. Mast cells are tissue-resident leukocytes known to influence a range of immune functions in a variety of different ways, largely through the secretion of biologically active mediators from preformed granules. However, it is not understood how mast cells influence the inflammatory environment in sickle cell disease. A notable consequence of sickle cell disease is severe pain. Therefore, morphine is often used to treat this disease. Because mast cells express opioid receptors, it is pertinent to understand how chronic morphine exposure influences mast cell function and inflammation in sickle cell disease. Herein, carbon-fiber microelectrode amperometry (CFMA) was used to monitor the secretion of immunoactive mediators from single mast cells. CFMA enabled the detection and quantification of discrete exocytotic events from single mast cells. Mast cells from two transgenic mouse models expressing human sickle hemoglobin (hBERK1 and BERK) and a control mouse expressing normal human hemoglobin (HbA-BERK) were monitored using CFMA to explore the impact of sickle cell-induced inflammation and chronic morphine exposure on mast cell function. This work, utilizing the unique mechanistic perspective provided by CFMA, describes how mast cell function is significantly altered in hBERK1 and BERK mice, including decreased serotonin released compared to HbA-BERK controls. Furthermore, morphine was shown to significantly increase the serotonin released from HbA-BERK mast cells and demonstrated the capacity to reverse the observed sickle cell-induced changes in mast cell function.

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“…To study mechanisms regulating stimulus-secretion coupling and to perform functional analysis of exocytosis at cellular and sub-cellular level, there have been great interests in developing methods and probes for monitoring specific exocytotic events in the secretory cells of interest [5]. Traditional electrical methods including patch-clamp capacitance measurement or electrochemical methods such as carbon fiber amperometry are capable of monitoring regulated secretion at the cellular level with high temporal resolution [6, 7], yet they are limited in their spatial resolution and can only be applied to a single cell at a time. Fluorescence microscopy, on the other hand, provides a number of benefits including high sensitivity of detection, high spatial and temporal resolution, and the ability of monitoring multiple cells simultaneously in cell populations including tissues or organs.…”

Section: Introductionmentioning

confidence: 99%

Probes for monitoring regulated exocytosis

2017

Cell Calcium

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Regulated secretion is a fundamental cellular process that serves diverse functions in neurobiology, endocrinology, immunology, and numerous other aspects of animal physiology. In response to environmental or biological cues, cells release contents of secretory granules into extracellular medium to communicate with or impact neighboring or distant cells through paracrine or endocrine signaling. To investigate mechanisms governing stimulus-secretion coupling, to better understand how cells maintain or regulate their secretory activity, and to characterize secretion defects in human diseases, probes for tracking various exocytotic events at the cellular or sub-cellular level have been developed over the years. This review summarizes different strategies and recent progress in developing optical probes for monitoring regulated secretion in mammalian cells.

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Microelectrodes for studying neurobiology

Cited by 45 publications

References 44 publications

Reference values of the chronoamperometric response at cylindrical and capped cylindrical electrodes

Reference values of the chronoamperometric response at cylindrical and capped cylindrical electrodes

Carbon-Fiber Microelectrode Amperometry Reveals Sickle-Cell-Induced Inflammation and Chronic Morphine Effects on Single Mast Cells

Probes for monitoring regulated exocytosis

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