Microelectrode array in mixed alkanethiol self-assembled monolayers: Electrochemical studies

Cancino, Juliana; Machado, Sérgio Antônio Spínola

doi:10.1016/j.electacta.2012.04.009

Cited by 22 publications

(10 citation statements)

References 41 publications

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“…The procedure for the immobilization of biomolecules is essential, since the species to be immobilized must keep their properties for recognition, to develop an efficient SPR-based immunosensor. Thus, the use of self-assembled monolayers (SAMs) as an initial step for preparation of biosensors has provided greater sensitivity and stability to these systems (Cancino and Machado, 2012).…”

Section: Introductionmentioning

confidence: 99%

Endurance training blocks uncoupling protein 1 up-regulation in brown adipose tissue while increasing uncoupling protein 3 in the muscle tissue of rats fed with a high-sugar diet

et al. 2012

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The mitochondrial uncoupling proteins (UCPs) of interscapular brown adipose tissue (iBAT) and of muscles play important roles in energy balance. For instance, the expression of UCP1 and UCP3 are modulated by free fatty acid gradients induced by high-sugar diets and acute exercise that is dependent on sympathetic stimulation. However, the effects of endurance training in animals fed with high-sugar diets are unknown. This study aims to evaluate the long-term effects of diet and exercise on UCP1 and UCP3 levels and energy balance efficiency. Rats fed with standard or high-sugar (HSD) diets were simultaneously subjected to running training over an 8-week period. After the training period, the rats were decapitated, and the iBAT and gastrocnemius muscle tissues were removed for evaluation of the β₃-receptor, Ucp1, and Ucp3 mRNA and protein expression, which were analyzed by quantitative reverse transcriptase polymerase chain reaction and Western blot, respectively. Groups fed with an HSD displayed a higher adiposity index and iBAT weight (P < .05), whereas exhibited an up-regulation of Ucp1 mRNA and protein levels (P < .05). Training increased β₃-receptor mRNA in iBAT and reduced the Ucp3 mRNA in muscle tissues. In association with an HSD, training restored the increasing β₃-receptor mRNA and greatly up-regulated the levels of Ucp3 mRNA. Therefore, training blocked the HSD-induced up-regulation of UCP1 expression in iBAT, whereas it up-regulated the expression of Ucp3 mRNA in muscle. These results suggest that training enhances the relationship between Ucp1/Ucp3 mRNA levels, which could result in higher energy efficiency, but not when HSD-induced elevated sympathetic activity is maintained.

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Section: Introductionmentioning

confidence: 99%

Endurance training blocks uncoupling protein 1 up-regulation in brown adipose tissue while increasing uncoupling protein 3 in the muscle tissue of rats fed with a high-sugar diet

et al. 2012

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“…The voltammetric profile for a bare interdigitated electrode is typical of an electron-transfer mechanism. 24 However, after the SAM adsorption formed by 11-AMU thiol, the oxidation current decreases, which suggests that the thiol blocking monolayer is adsorbed. The remaining oxidation current may be related to defects in the thiol monolayer adsorption.…”

Section: Resultsmentioning

confidence: 99%

Nanostructured Sensors Containing Immobilized Nuclear Receptors for Thyroid Hormone Detection

Bendo¹,

Figueira²,

Polikarpov³

et al. 2014

j biomed nanotechnol

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Thyroid hormone receptors (TRs) are members of the nuclear receptors (NRs) superfamily, being encoded by two genes: TRa and TRbeta. In this paper, the ligand-binding domain (LBD) of the TRbeta1 isoform was immobilized on the surface of nanostructured electrodes for TR detection. The platforms containing TRbeta1-LBD were applied to the detection of specific ligand agonists, including the natural hormones T3 (triiodothyronine) and T4 (thyroxine), and the synthetic agonists TRIAC (3,5,3'-triiodothyroacetic acid) and GC-1 [3,5-dimethyl-4-(4'-hydroxy-3'-isopropylbenzyl phenoxy) acetic acid]. Detection was performed via impedance spectroscopy. The biosensors were capable of distinguishing between the thyroid hormones T3 and T4, and/or the analogues TRIAC and GC-1 at concentrations as low as 50 nM. The detection and separation of thyroid hormones and analogue ligands by impedance techniques represents an innovative tool in the field of nanomedicine because it allows the design of inexpensive devices for the rapid and real-time detection of distinct ligand/receptor systems.

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“…133 Apart from single molecule type SAM, mixed SAMs have been developed to incorporate two types of molecules in one SAM where one type of species would preclude the other by steric hindrance. 91 Mixed monolayers have great potential in many different applications including microelectrodes, 134 separation of biomolecules, 135 environmental monitoring, 136 biosensors 137 and tribology. 63 Feng et al developed a mixed SAM containing both OTS and octyltriethoxysilane, see a schematic of the mixed SAM in Fig.…”

Section: Achievements and Applications Of Samsmentioning

confidence: 99%

Challenges and developments of self-assembled monolayers and polymer brushes as a green lubrication solution for tribological applications

et al. 2015

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ab Self-assembled monolayers (SAMs), after originally being investigated due to their functions in changing surface wettability, have been significantly developed over the years. Many types of SAMs have been developed on a variety of substrates. However their formation mechanism, rate and quality are found to be influenced by many factors. A range of SAMs including single-and multi-component are included in this review with focus on the nano and macro tribological properties. More recently, surface initiated polymer brushes, i.e. macromolecular assemblies attached to a substrate, have emerged to be an alternative and promising method for surface modification. The ability to tether these macromolecules to tribological contacts is key to their resistance to shear under loaded contacts. This review also covers atom transfer radical polymerisation (ATRP) and the role of this technique in developing new lubrication solutions. Particular care has been taken to include the development of lubrication solutions for silicon nitride due to the importance of this material as an engineering ceramic. This paper reviews the stateof-the-art development of SAMs and polymer brushes especially the potential opportunities and challenges in applying them in tribological contacts as a lubrication solution.

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Microelectrode array in mixed alkanethiol self-assembled monolayers: Electrochemical studies

Cited by 22 publications

References 41 publications

Endurance training blocks uncoupling protein 1 up-regulation in brown adipose tissue while increasing uncoupling protein 3 in the muscle tissue of rats fed with a high-sugar diet

Endurance training blocks uncoupling protein 1 up-regulation in brown adipose tissue while increasing uncoupling protein 3 in the muscle tissue of rats fed with a high-sugar diet

Nanostructured Sensors Containing Immobilized Nuclear Receptors for Thyroid Hormone Detection

Challenges and developments of self-assembled monolayers and polymer brushes as a green lubrication solution for tribological applications

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