Microelectrical sensors as emerging platforms for protein biomarker detection in point-of-care diagnostics

Arruda, David L; Wilson, William C.; Nguyen, Crystal; Yao, Qi W; Caïazzo, Robert; Talpasanu, Ilie; Dow, Douglas E.; Liu, Brian C-S

doi:10.1586/erm.09.47

Cited by 45 publications

(34 citation statements)

References 40 publications

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“…The detection of insulin in diabetes samples has been used in early-diagnosis, monitoring disease progression, prognosis and pathology research [2]. The progression of technique for insulin analysis will undoubtedly improve the early diagnosis for relative disease and facilitate the follow-up therapy [3, 4]. …”

Section: Introductionmentioning

confidence: 99%

Immune-enrichment of insulin in bio-fluids on gold-nanoparticle decorated target plate and in situ detection by MALDI MS

Liang

2017

Clin Proteom

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BackgroundDetection of low-abundance biomarkers using mass spectrometry (MS) is often hampered by non-target molecules in biological fluids. In addition, current procedures for sample preparation increase sample consumption and limit analysis throughput. Here, a simple strategy is proposed to construct an antibody-modified target plate for high-sensitivity MS detection of target markers such as insulin, in biological fluids.MethodsThe target plate was first modified with gold nanoparticle, and then functionalized with corresponding antibody through chemical conjugation. Clinical specimens were incubated onto these antibody-functionalized target plates, and then subjected to matrix assisted laser desorption ionization mass spectrometry analysis.ResultsInsulin in samples was enriched specifically on this functional plate. The detection just required low-volume samples (lower than 5 µL) and simplified handling process (within 40 min). This method exhibited high sensitivity (limit of detection in standard samples, 0.8 nM) and good linear correlation of MS intensity with insulin concentration (R2 = 0.994). More importantly, insulin present in real biological fluids such as human serum and cell lysate could be detected directly by using this functional target plate without additional sample preparations.ConclusionsOur method is easy to manipulate, cost-effective, and with a potential to be applied in the field of clinical biomarker detection.

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Section: Introductionmentioning

confidence: 99%

Immune-enrichment of insulin in bio-fluids on gold-nanoparticle decorated target plate and in situ detection by MALDI MS

Liang

2017

Clin Proteom

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“…The amplitude and phase of the transmitted SAW with respect to the incoming one is detected by a second IDT. The differences in amplitude and phase are connected to changes in the mass load on the sensing area, viscosity, temperature or even conductivity on the surface of the sensing substrate [27]. With increasing frequency between 25 and 500 MHz, the penetration depth decreases, making the changes in the surface boundary conditions more important.…”

Section: Actuation By Surface Acoustic Wavesmentioning

confidence: 99%

Surface Acoustic Wave Actuated Lab-on-Chip System for Single Cell Analysis

Thalhammer

Wixforth²

2013

J Biosens Bioelectron

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Collection, selection, amplification and detection of minimal amounts of biological samples became part of everyday life in medical and biological laboratories. The goal of the lab-on-a-chip technology is to automate standard laboratory processes and to analyze smallest samples reproducibly and reliably in a miniaturized format. Until now, however, microfluidic devices are not yet standardized equipment found in biologists' toolboxes. Just one of the obstacles is the difficulty of producing them. We review various approaches for lab-on-chip systems reported in the literature but focus on our own system, based on surface acoustic wave (SAW) actuation. Finally, we present a freely programmable planar chip system actuated by this operating principle and show that it has several advantages over 'conventional' microfluidic channel systems. Apart from avoiding the notorious problems of clogging, large pressure drops, and large surface area, our approach minimizes the risk of contamination and loss of analyte. Entering pointof-care diagnosis, the necessity of single cell analysis is dramatically increasing. To optimize a tumor therapy, for example, knowledge of the genetic composition of the heterogenic tumor tissue is essential. Hence, there exists the need for a reliable analysis system, which can be easily adapted to altering problems without reduction in reliability and reproducibility.

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“…Both SAW delay lines will operate at the same temperature. Figure 11 illustrates a SAW biosensor in a dual delay line configuration (Arruda et al, 2009). Measurements comparing the experimental delay line with the reference delay line are used to compensate the effect of temperature on the biosensor.…”

Section: Saw Delay Lines As Biosensorsmentioning

confidence: 99%