Microdistribution of substratum-bound ligands affects cell function: hepatocyte spreading on PEO-tethered galactose

Griffith, Linda G.; Lopina, Stephanie T.

doi:10.1016/s0142-9612(97)00185-3

Cited by 104 publications

(60 citation statements)

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“…Surface density of the cell adhesive ligands immobilized onto polymer substrates plays an important role in controlling the extent of cell adhesion and cell morphology (21)(22)(23)(24). Densely populated cell adhesive ligands on the surface induce the clustering of cell membrane receptors, leading to enhanced cell spreading and adhesion (25,26). When the temperature was lowered to 30°C, the tightly adhered chondrocytes on the collapsed beads may be rapidly detached by the drastically reduced surface density of the RGD moiety upon swelling.…”

Section: Resultsmentioning

confidence: 99%

Swelling Induced Detachment of Chondrocytes Using RGD‐Modified Poly(N‐isopropylacrylamide) Hydrogel Beads

Kim

Jeong

Park

2002

Biotechnology Progress

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Thermally sensitive poly(N-isopropylacrylamide, NIPAAm) hydrogel beads conjugated with a cell adhesive motif, GRGDY, were prepared and utilized as cell culture substrate for chondrocytes. They were produced to be uniform in size and distribution by using calcium alginate as a temporal mold. The RGD moieties were introduced, in a spatially selective manner, to the surface of the beads by conjugating GRGDY under the precollapsed state at a higher temperature above the lower critical solution temperature (LCST). These RGD-conjugated polyNIPAAm beads demonstrated a reversible swelling and deswelling behavior around the LCST, which enabled the chondrocytes attached on the surface of collapsed beads at 37 degrees C to readily detach when the temperature was shifted below 37 degrees C. The cell detachment percentage was largely affected by the temperature-dependent reswelling extent of the collapsed RGD-modified beads.

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Section: Resultsmentioning

confidence: 99%

Swelling Induced Detachment of Chondrocytes Using RGD‐Modified Poly(N‐isopropylacrylamide) Hydrogel Beads

Kim

Jeong

Park

2002

Biotechnology Progress

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“…Most of these studies have been devoted to the immobilization of galactose or galactose derivatives to promote adhesion of hepatocytes to substrata, which exploits the binding of galactose to the asialoglycoprotein receptor of hepatocytes. [ 173 ] Such surface coating provides also selectivity because co-culture studies with hepatocytes and fi broblasts show preferential adhesion and growth of hepatocytes on galactose-modifi ed surface areas, while attachment of fi broblasts to the hydrophilic glycan-coating was inhibited. [ 174 ] Further studies were conducted to explore the potential of other glycans to guide adhesion of cells, such as mannose and lactose that promoted adhesion and growth of chondrocytes, while adhesion and growth of chondrosarcoma cells were inhibited.…”

Section: Sweet Spots: Glycomimetics In Tissue Engineeringmentioning

confidence: 99%

Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

et al. 2010

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Cell fate is regulated by extracellular environmental signals. Receptor specific interaction of the cell with proteins, glycans, soluble factors as well as neighboring cells can steer cells towards proliferation, differentiation, apoptosis or migration. In this review, approaches to build cellular structures by engineering aspects of the extracellular environment are described. These methods include non-specific modifications to control the wettability and stiffness of surfaces using self-assembled monolayers (SAMs) and polyelectrolyte multilayers (PEMs) as well as methods where the temporal activation and spatial distribution of adhesion ligands is controlled. Building on these techniques, construction of two-dimensional cell sheets using temperature sensitive polymers or electrochemical dissolution is described together with current applications of these grafts in the clinical arena. Finally, methods to pattern cells in three-dimensions as well as to functionalize the 3D environment with biologic motifs take us one step closer to being able to engineer multicellular tissues and organs.

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“…Some types of sugar-derivatized polystyrenes were synthesized to cultivate hepatocytes [1], and it was reported that a galactosylated surface is an attractive substrate for hepatocyte culture because of the specific interaction between the galactose ligand and the asialoglycoprotein receptor on hepatocytes [2][3][4]. In addition, polysaccharides such as chitin [5], chitosan [6][7][8][9][10], heparin [9][10][11], alginate [12][13][14], and pullulan [15,16] have been derivatized for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of photoreactive pullulan for surface modification

et al. 2005

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Microdistribution of substratum-bound ligands affects cell function: hepatocyte spreading on PEO-tethered galactose

Cited by 104 publications

References 0 publications

Swelling Induced Detachment of Chondrocytes Using RGD‐Modified Poly(N‐isopropylacrylamide) Hydrogel Beads

Swelling Induced Detachment of Chondrocytes Using RGD‐Modified Poly(N‐isopropylacrylamide) Hydrogel Beads

Engineering the Extracellular Environment: Strategies for Building 2D and 3D Cellular Structures

Synthesis of photoreactive pullulan for surface modification

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