Microdialysis of large molecules

Clough, Geraldine F.

doi:10.1208/aapsj070369

Cited by 113 publications

(87 citation statements)

References 60 publications

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“…biofouling) and whether depletion of target molecules in the immediate area around the membrane occurs (20)(21)(22)(23)(24). This study complements previously published data on CMD findings after catheter insertion (18) and allows discussion of these questions within that time period, which includes axon reflex-mediated mechanisms, skin reactivity mechanisms and "biofouling".…”

Section: Introductionsupporting

confidence: 65%

“…As we and others have found, passage of cytokines through the PES membrane is not only dependent upon the molecular weight but is also far more complex and includes the actual structure, charge and other molecule characteristics of the proteins (17,18,25,35). Much of this work is of common interest for all researchers using microdialysis.…”

Section: This Comparison Of Microdialysis (Cmd) and If Findingsmentioning

confidence: 99%

“…Results for the 1-h sample immediately prior to biopsy will be shown in this paper. Other time points in the microdialysis analysis are previously published (18). …”

Section: Microdialysate Testing For Cytokine Contentmentioning

confidence: 99%

“…Detailed findings on the 24-h chronology of cytokine production are available in a previous publication (18) and are summarised in regard to their detection or not at an individual subject level in Fig. 1.…”

Section: Microdialysate Analysismentioning

confidence: 99%

“…The issues of tissue reactivity, levels of trauma and recovery (equilibration) times are central to any discussion of CMD. This is especially so in the interpretation of findings on cytokines and other biologically active molecules such as chemokines and growth factors (9,10,(17)(18)(19), which patently have longer "equilibration" times than axon reflex-mediated microvascular events (11)(12)(13). Other central issues concern whether membranes retain their laboratory bench determined function in relation to passage of target molecules in vivo in the short-(e.g.…”

Section: Introductionmentioning

confidence: 99%

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Are Cutaneous Microdialysis Cytokine Findings Supported by End Point Biopsy Immunohistochemistry Findings?

Sjögren

Anderson

2010

AAPS J

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Abstract. Insertion of a cutaneous microdialysis catheter into normal dermis has been shown to induce the production of IL1b, IL6 and IL8 in an innate response to minimal trauma. In the present study, skin biopsy for immunohistochemistry has been performed at the site of the microdialysis catheter to compare the findings with that of the microdialysis findings 24 h after insertion. Of the three named cytokines, concordance between the two investigated technologies was highest for IL8 (100%) followed by IL6 (70%) and IL1b (50%). For seven other pro-inflammatory and T cell-relevant cytokines studied, concordance ranged between 50% and 80%. The total number of positive (microdialysis or immunofluorescence) findings was similar between the two methodologies. Technical and biological phenomenon can explain the differences. We conclude that both methodologies illustrate important features of tissue biology and that a combination of the two methods in clinical research can provide the chronology of soluble mediator participation and the more classic, but also more invasive, biopsy-based methodology at a point which constitutes the end of the observation period. We conclude further that at the 24-h time period here studied, microdialysis catheters are still functional and thus capable of producing relevant data which can be corroborated and extended by the "end point biopsy".

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Section: Introductionsupporting

confidence: 65%

Section: This Comparison Of Microdialysis (Cmd) and If Findingsmentioning

confidence: 99%

“…Results for the 1-h sample immediately prior to biopsy will be shown in this paper. Other time points in the microdialysis analysis are previously published (18). …”

Section: Microdialysate Testing For Cytokine Contentmentioning

confidence: 99%

Section: Microdialysate Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Are Cutaneous Microdialysis Cytokine Findings Supported by End Point Biopsy Immunohistochemistry Findings?

Sjögren

Anderson

2010

AAPS J

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Microdialysis Sampling

Stenken¹

2006

Encyclopedia of Medical Devices and Instrumentation

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Microdialysis samplin is a well know in vivo collection method for low molecular weight hydrophilic analytes. This sampling method has its historical roots in the field of neuroscience since it is commonly used for collection of neurotransmitters. The successful application of microdialysis sampling for neuroscience applications has led to the expansion of the technique for collection of a wider range of analytes including drugs, metabolites, peptides and proteins. This article covers both the practical and theoretical aspects of in vivo microdialysis sampling. A brief comparison of the advantages and disadvantages of a few in vivo analysis methods versus microdialysis sampling is presented. The fundamental priciples of microdialysis sampling are disucssed with an emphasis on how analyte diffusive mass transport affects the recovery process during microdialysis sampling. This understanding of diffusive mass transport is especially critical during the in vivo recovery of larger peptides and proteins. Next, sample analysis challenges and needs are described since only low microliter volumes are obtained during the microdialysis sampling process. The many different types of analytical methods used for analyte quantitation are presented including immunoassays, sensors and separations methods. Both clinical and research applications of the technique are also described. Finally, a vision for the future of microdialysis sampling and recommendations for deveice and methodology improvement are proposed.

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Membrane‐Based Separations Applied toin VivoGlucose Sensing—Microdialysis and Ultrafiltration Sampling

Stenken

2009

In Vivo Glucose Sensing

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Microdialysis of large molecules

Cited by 113 publications

References 60 publications

Are Cutaneous Microdialysis Cytokine Findings Supported by End Point Biopsy Immunohistochemistry Findings?

Are Cutaneous Microdialysis Cytokine Findings Supported by End Point Biopsy Immunohistochemistry Findings?

Microdialysis Sampling

Membrane‐Based Separations Applied toin VivoGlucose Sensing—Microdialysis and Ultrafiltration Sampling

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