Microdialysis Sampling

Stenken, Julie A.

doi:10.1002/0471732877.emd330

Cited by 7 publications

(7 citation statements)

References 189 publications

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“…[15,16] Microdialysis is a well established membrane-based technique in neurosciences and pharmacokinetic studies for in-vivo sampling of endogenous or xenobiotic compounds in discrete compartments of living systems. [17][18][19][20] Yet, current trends have been directed to expand the scope of this technique to the monitoring of bioprocesses (e.g. fermentations), [21,22] the assessment of the quality of foodstuffs [23][24][25] and cosmetics [26,27] and the probing of target species in environmentally relevant matrices.…”

Section: Introductionmentioning

confidence: 99%

In-situ sampling of soil pore water: evaluation of linear-type microdialysis probes and suction cups at varied moisture contents

Miró

Fitz²,

Swoboda³

et al. 2010

Environ. Chem.

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Environmental context. There is a need for slightly invasive techniques capable of in-situ probing of target analytes in environmental compartments. Owing to its passive sampling mode and small probe dimensions, microdialysis-based dosimetry is an appealing tool for monitoring of solute concentrations in both water bodies and pore soil waters with minimum disturbance of natural equilibrium. The development of field applications is challenging but will provide novel insights as to the speciation and bioaccessibility of environmental pollutants, e.g. trace metals, at high spatial resolution.Abstract. In-situ sampling of soil pore water is still a challenge for environmental scientists. Here, microdialysis is explored for probing metal concentrations in soil pore water at soil moisture contents ranging from 50 to 115% of the maximal water holding capacity and is compared with traditional sampling by suction cups. Metal concentrations obtained by the suction cup technique were consistently larger than those measured in the dialysate. Good agreement was obtained for Pb and Cu at soil moistures close to saturation after accounting for diffusion resistances whereas corrected Ni and Cd concentrations in the dialysates exceeded those measured by the suction cup technique. These deviations reflect inherent differences in the sampling mode and effects of soil heterogeneity at the microscale. Microdialysis offers new opportunities to probe solute concentrations at high spatial resolution and minimal disturbance of soil conditions at environmental interfaces such as the plant rhizosphere or at the transition between forest floors and the mineral soil.

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Section: Introductionmentioning

confidence: 99%

In-situ sampling of soil pore water: evaluation of linear-type microdialysis probes and suction cups at varied moisture contents

Miró

Fitz²,

Swoboda³

et al. 2010

Environ. Chem.

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“…We chose to use a higher perfusion fluid flow rate through the dialysis probe to obtain more analyte mass and thus use the sample preparation process as a means to concentrate the collected dialysates. For low molecular weight solutes, mass recovery in microdialysis sampling increases with increasing flow rates. − …”

Section: Resultsmentioning

confidence: 99%

Microdialysis Sampling of Quorum Sensing Homoserine Lactones during Biofilm Formation

et al. 2019

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Bacteria communicate chemically through a system called quorum sensing. In this work, microdialysis sampling procedures were optimized to collect quorum sensing molecules produced during in situ biofilm formation directly on the polymeric semipermeable membrane of the microdialysis probe. V. harveyi, a Gram-negative bacterium, was used as the model organism and releases variable chain length acylhomoserine lactones (AHLs) and acyl-oxohomoserine lactones (AOHLs) as signaling molecules during quorum sensing. Eliciting biofilm formation required coating fetal bovine serum onto the poly(ether sulfone) microdialysis membrane. Dialysates were collected in different experiments either during or after biofilm formation directly on a microdialysis probe. Continuous sampling of C4-AHL, C6-AHL, C8-AHL, C6-OXO-AHL, and C12-OXO-AHL was achieved over a period of up to 4 days. The AHLs and AOHLs in dialysates were concentrated with solid-phase extraction and quantified using LC-MS. Dialysate concentrations obtained for the AOHLs and AHLs ranged between 1 and 100 ppb (ng/mL) and varied between sampling days. This work demonstrates the initial use of microdialysis sampling to collect quorum sensing signaling chemicals during biofilm formation by a Gram-negative bacterial species.

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“…Microdialysis is a diffusion-based sampling method that has been used for four decades as an established research tool in life science and biomedical research. − A perfusion fluid is passed through the device at microliter per minute flow rates, which induces the diffusion of analytes from the sample medium into the probe according to their concentration gradient. Since microdialysis relies on the same mode of action as plant roots during nutrient acquisition (diffusion), soil N fluxes during plant uptake can be measured reliably by employing microdialysis in the rhizosphere. , Due to the minimally invasive nature and small size of microdialysis, it is suitable for sampling soil microsites and providing real-time, in situ data with greater temporal and spatial resolutions than current standard, salt-solution extraction methods.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen Dynamics: Quantifying and Differentiating Fluxes in a Riparian Wetland Soil

Maddala

Savin

Stenken

et al. 2021

ACS Earth Space Chem.

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Excess nitrogen (N) originating from stormwater runoff in urban areas comes from diffuse sources and is difficult to manage and remediate. One site affected by nonpoint source pollution is Lake Keith, located in Cave Springs, Arkansas, which contains water rich in nitrate-N (5−6 mg N/L). Microdialysis, a diffusion-based sampling technique, has been recognized as a tool for collecting samples from and monitoring chemical changes in soil microsites such as the rhizosphere. Successfully using microdialysis in soil obtained from Lake Keith, an anthropogenically disturbed location that differs in physical and chemical characteristics from the soils studied in past microdialysis studies, will add to the applicability of the sampling technique and promote its establishment as a conventional soil sampling method. In this study, microdialysis was employed to investigate the dynamics of nitrogen in (1) unvegetated soil at different soil saturations and (2) in the rhizosphere of native plant speciesSwitchgrass (Panicum virgatum) and Reed canarygrass (Phalaris arundinacea). Diffusive fluxes in the Switchgrass treatment were either minimal or undetectable, and these data were complemented by the significantly decreased concentrations of nitrate-N and total N in the soil and the accumulation of plant biomass N and total biomass production. On the contrary, the Reed canarygrass treatment consistently exhibited the greatest fluxes of nitrate-N, along with decreased biomass growth. Ultimately, the microdialysis technique differentiated nitrogen dynamics in differing water saturation conditions and in the rhizospheres of two native plants, varying by functional wetland indicator status under the conditions provided by the greenhouse setting.

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Microdialysis Sampling

Cited by 7 publications

References 189 publications

In-situ sampling of soil pore water: evaluation of linear-type microdialysis probes and suction cups at varied moisture contents

In-situ sampling of soil pore water: evaluation of linear-type microdialysis probes and suction cups at varied moisture contents

Microdialysis Sampling of Quorum Sensing Homoserine Lactones during Biofilm Formation

Nitrogen Dynamics: Quantifying and Differentiating Fluxes in a Riparian Wetland Soil

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