Microdevices for separation, accumulation, and analysis of biological micro- and nanoparticles

Kentsch, Jörg; Dürr, Manfred; Schnelle, Thomas; Gradl, Gabriele; Müller, Torsten; Jäger, Magnus; Normann, Andrea; Stelzle, Martin

doi:10.1049/ip-nbt:20031127

Cited by 41 publications

(33 citation statements)

References 25 publications

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“…In 2003 this research group extended their work to hepatitis A virus (HAV) [46], which is an enveloped virus with a diameter around 27 nm. They employed a novel approach for viral detection, since instead of trapping the viral particles they utilized antibody coated beads with hepatitis virus bonded to their surface (Fig.…”

Section: Führ Stelzle and Co-workersmentioning

confidence: 99%

“…The system had the potential to be adapted to handle a variety of samples. A detailed description of the fabrication process of Nano VirDetec is shown in the original publication [46]. Briefly, the system chamber was composed by two glass slides (1 mm61 mm, 0.17 mm thick).…”

Section: Führ Stelzle and Co-workersmentioning

confidence: 99%

“…Particles with a lower dielectrophoretic force exerted on them, will continue flowing in the main channel, toward the fluid outlet 1. The operating conditions and microsystem dimensions could be manipulated in order to [46] to sort antibody coated beads with the hepatitis virus bonded to their surface.…”

Section: Führ Stelzle and Co-workersmentioning

confidence: 99%

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Dielectrophoresis for the manipulation of nanobioparticles

2007

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Dielectrophoresis (DEP) is a nondestructive electrokinetic mechanism with great potential for the manipulation of bioparticles. DEP is the movement of particles induced by polarization effects in nonuniform electric fields. Since the 1960s, this technique has been successfully used for the manipulation of microbioparticles, such as microorganisms. Moreover, due to the advances in microfabrication techniques, that allowed progressively smaller microstructures to be constructed, DEP can now be used for the manipulation of nanobioparticles. The first research studies on the DEP of nanobioparticles started in the 1990s. Since then, many research groups have carried out outstanding work with DEP of nanobioparticles such as macromolecules, virus, and spores. However, the need of a critical report that integrates these findings is evident. The aim of the present review is to depict the state-of-the-art on the use of DEP for the separation of nanobioparticles and the potential trends of novel applications of this technique. This review compiles and analyzes the significant findings obtained by many researchers. This publication is intended to provide the reader with state-of-the-art information on many research studies focused on DEP to handle nanobioparticles.

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Section: Führ Stelzle and Co-workersmentioning

confidence: 99%

Section: Führ Stelzle and Co-workersmentioning

confidence: 99%

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Dielectrophoresis for the manipulation of nanobioparticles

2007

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“…Therefore, various target cells with different dielectric properties can be manipulated by controlling the medium properties or the input voltage condition. In order to manipulate biological particles, consequently, various dielectrophoresis-based techniques, including DEP trapping, DEP field-flow fractionation (DEP-FFF), traveling wave DEP (TwDEP) force and DEP barrier, are performed within a micro fluid channel [13][14][15][16][17][18][19][20][21]. DEP trapping techniques are mainly used to isolate particles within a still fluid utilizing p-DEP force [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

The potential of a dielectrophoresis activated cell sorter (DACS) as a next generation cell sorter

Lee

Hwang

Kim

2016

Micro and Nano Syst Lett

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Originally introduced by H. A. Pohl in 1951, dielectrophoretic (DEP) force has been used as a striking tool for biological particle manipulation (or separation) for the last few decades. In particular, dielectrophoresis activated cell sorters (DACSes) have been developed for applications in various biomedical fields. These applications include cell replacement therapy, drug screening and medical diagnostics. Since a DACS does not require a specific bio-marker, it is able to function as a biological particle sorting tool with numerous configurations for various cells [e.g. red blood cells (RBCs), white blood cells (WBCs), circulating tumor cells, leukemia cells, breast cancer cells, bacterial cells, yeast cells and sperm cells]. This article explores current DACS capabilities worldwide, and it also looks at recent developments intended to overcome particular limitations. First, the basic theories are reviewed. Then, representative DACSes based on DEP trapping, traveling wave DEP systems, DEP field-flow fractionation and DEP barriers are introduced, and the strong and weak points of each DACS are discussed. Finally, for the purposes of commercialization, prerequisites regarding throughput, efficiency and recovery rates are discussed in detail through comparisons with commercial cell sorters (e.g. fluorescent activated and magnetic activated cell sorters).

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“…Another flow separator using 3D arrays of electrodes embedded in microchannels, so-called "deflector" structures ͑electrodes oriented at certain angles compared with the flow direction͒, is presented in Ref. 24. Our previous work presented a few dielectrophoretic separation methods in DEP devices with 3D electrodes.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic field-flow method for separating particle populations in a chip with asymmetric electrodes

Iliescu

Tresset

2009

Biomicrofluidics

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This paper presents a field-flow method for separating particle populations in a dielectrophoretic ͑DEP͒ chip with asymmetric electrodes under continuous flow. The structure of the DEP device ͑with one thick electrode that defines the walls of the microfluidic channel and one thin electrode͒, as well as the fabrication and characterization of the device, was previously described. A characteristic of this structure is that it generates an increased gradient of electric field in the vertical plane that can levitate the particles experiencing negative DEP. The separation method consists of trapping one population to the bottom of the microfluidic channel using positive DEP, while the other population that exhibits negative DEP is levitated and flowed out. Viable and nonviable yeast cells were used for testing of the separation method.

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Microdevices for separation, accumulation, and analysis of biological micro- and nanoparticles

Cited by 41 publications

References 25 publications

Dielectrophoresis for the manipulation of nanobioparticles

Dielectrophoresis for the manipulation of nanobioparticles

The potential of a dielectrophoresis activated cell sorter (DACS) as a next generation cell sorter

Dielectrophoretic field-flow method for separating particle populations in a chip with asymmetric electrodes

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