Microcystin-LR induces abnormal root development by altering microtubule organization in tissue-cultured common reed (Phragmites australis) plantlets

Máthé, Csaba; Beyer, Dániel; Erdõdi, Ferenc; Serfözö, Zoltán; Székvölgyi, Lóránt; Vasas, Gábor; M-Hamvas, Márta; Jámbrik, Katalin; Gonda, Sándor; Kiss, Andrea

doi:10.1016/j.aquatox.2009.02.005

Cited by 51 publications

(43 citation statements)

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“…The majority of investigations into the effects of cyanobacterial toxins on plants to date have focused on individual microcystin variants (Abe et al 1996;McElhiney et al 2001;Máthé et al 2009). However, there is significant difference in the toxicities of MC-RR, -LR, and -LF on the growth of mustard (Synapis alba) seedlings (McElhiney et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila)

et al. 2010

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The bioaccumulation and harmful effects of microcystins (MCs) and the activity of peroxidase (POD) and superoxide dismutase (SOD) were examined in the apple (Malus pumila) exposed in vitro with the crude extract of toxic cyanobacterial blooms from Dianchi Lake in southwestern China. The results showed that the growth and proliferation of M. pumila shoots in vitro decreased markedly after exposure to microcystins above 0.3 microg/ml. Recovered microcystins determined by enzyme-linked immunosorbent assay (ELISA) in M. pumila shoot cultures increased with exposure time and concentration. After 14 days exposure to the concentration of 3 microg/ml microcystins, M. pumila shoot cultures accumulated microcystins up to a concentration of 510.23 +/- 141.10 ng MC-LR equiv/g FW (fresh weight), equivalent to an accumulation rate of 36.45 ng/g day. POD activity was significantly increased after 7 days exposure to 3 microg/ml microcystins. After 14 days of exposure, microcystins caused POD to increase significantly at the concentration of 0.3 and 3 microg/ml. The activity of SOD was not affected by microcystins at concentrations up to 3 microg/ml on 7 days. After 14 days exposure to microcystins, SOD activity increased significantly at the concentration of 0.3 and 3 microg/ml in M. pumila shoot cultures.

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Section: Discussionmentioning

confidence: 99%

Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila)

et al. 2010

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“…Alterations in root architecture and development have been reported for tissue-cultured common reed (Phragmites australis) challenged with 0.5-40 lg/mL MC-LR. These effects may be attributed to the activity of MC-LR on microtubule organization (Máthé et al 2009). This work underlines the dose and time effects of exposure to the toxin.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, 2 day exposure to 10-40 lg/ml MC-LR caused inhibition of mitosis in roots of common reed (Phragmites australis). However, prolonged exposure (20 days) stimulated or inhibited mitosis in plants according to the toxin concentration, 0.1-5 or 20-40 lg/ml MC-LR, respectively (Máthé et al 2009). Research has also highlighted that plants may take up and accumulate MCs.…”

Section: Introductionmentioning

confidence: 99%

Early physiological and biochemical responses of rice seedlings to low concentration of microcystin-LR

Azevedo¹,

Azevedo

Osório

et al. 2013

Ecotoxicology

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Microcystin-leucine and arginine (microcystin-LR) is a cyanotoxin produced by cyanobacteria like Microcystis aeruginosa, and it's considered a threat to water quality, agriculture, and human health. Rice (Oryzasativa) is a plant of great importance in human food consumption and economy, with extensive use around the world. It is therefore important to assess the possible effects of using water contaminated with microcystin-LR to irrigate rice crops, in order to ensure a safe, high quality product to consumers. In this study, 12 and 20-day-old plants were exposed during 2 or 7 days to a M. aeruginosa extract containing environmentally relevant microcystin-LR concentrations, 0.26-78 lg/L. Fresh and dry weight of roots and leaves, chlorophyll fluorescence, glutathione Stransferase and glutathione peroxidase activities, and protein identification by mass spectrometry through twodimensional gel electrophoresis from root and leaf tissues, were evaluated in order to gauge the plant's physiological condition and biochemical response after toxin exposure. Results obtained from plant biomass, chlorophyll fluorescence, and enzyme activity assays showed no significant differences between control and treatment groups. How-ever, proteomics data indicates that plants respond to M. aeruginosa extract containing environmentally relevant microcystin-LR concentrations by changing their metabolism, responding differently to different toxin concentrations. Biological processes most affected were related to protein folding and stress response, protein biosynthesis, cell signalling and gene expression regulation, and energy and carbohydrate metabolism which may denote a toxic effect induced by M. aeruginosa extract and microcystin-LR. The implications of the metabolic alterations in plant physiology and growth require further elucidation.

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“…Studies on the effects of MC-LR in the organization of microtubules and chromatin in root cells of P. australis demonstrated that the induction of abnormal mitotic cells coincided with concentrations where inhibition of protein phosphatases was greater than 50% (> 500 mg L -1 ) (Máthé et al, 2009). …”

Section: Effects Of Microcystins On Aquatic Plantsmentioning

confidence: 99%

Phytotoxicity associated to microcystins: a review

et al. 2014

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Microcystins (MC) are the most studied toxins of cyanobacteria since they are widely distributed and account for several cases of human and animal poisoning, being potent inhibitors of the serine/threonine protein phosphatases 1 (PP1) and 2A (PP2A). The phosphatases PP1 and PP2A are also present in plants, which may also suffer adverse effects due to the inhibition of these enzymes. In aquatic plants, biomass reduction is usually observed after absorption of cyanotoxins, which can bioaccumulate in its tissues. In terrestrial plants, the effects caused by microcystins vary from inhibition to stimulation as the individuals develop from seedling to adult, and include reduction of protein phosphatases 1 and 2A, oxidative stress, decreased photosynthetic activity and even cell apoptosis, as well as bioaccumulation in plant tissues. Thus, the irrigation of crop plants by water contaminated with microcystins is not only an economic problem but becomes a public health issue because of the possibility of food contamination, and this route of exposure requires careful monitoring by the responsible authorities.Keywords: bioaccumulation, microcystins effects, crop plants, aquatic plants. Fitotocixidade associada às microcistinas: uma revisão ResumoMicrocistinas (MC) são as toxinas de cianobactérias mais estudadas, uma vez que são amplamente distribuídas e responsáveis por vários casos de intoxicação humana e animal. São potentes inibidoras das proteínas fosfatases serina/treonina 1 (PP1) e 2A (PP2A). As fosfatases PP1 e PP2A também estão presentes em plantas, as quais podem sofrer efeitos adversos devido à inibição dessas enzimas. Em plantas aquáticas, a redução da biomassa é geralmente observada após absorção de cianotoxinas que podem bioacumular em seus tecidos. Em plantas terrestres, os efeitos causados pelas microcistinas variam de inibição ao estímulo, como no desenvolvimento de plântulas ao estádio adulto, e incluem a redução de proteínas fosfatases 1 e 2A, estresse oxidativo, diminuição da atividade fotossintética e até mesmo apoptose celular, bem como a bioacumulação em tecidos de plantas. Assim, a irrigação de plantas cultivadas com água contaminada com microcistina não é apenas um problema econômico, mas torna-se um problema de saúde pública, devido à possibilidade de contaminação dos alimento, sendo uma via de exposição que requer um monitoramento cuidadoso por parte das autoridades responsáveis.Palavras-chave: bioacumulação, efeitos de microcistinas, plantas da agricultura, plantas aquáticas.

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Microcystin-LR induces abnormal root development by altering microtubule organization in tissue-cultured common reed (Phragmites australis) plantlets

Cited by 51 publications

References 53 publications

Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila)

Bioaccumulation of microcystin and its oxidative stress in the apple (Malus pumila)

Early physiological and biochemical responses of rice seedlings to low concentration of microcystin-LR

Phytotoxicity associated to microcystins: a review

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