Microcosm evaluation of the impact of oil contamination and chemical dispersant addition on bacterial communities and sediment remediation of an estuarine port environment

Louvado, António; Coelho, Francisco J. R. C.; Oliveira, Vanessa; Gomes, Hélder; Cleary, Daniel F. R.; Simões, Mário M. Q.; Gomes, Newton C.M.

doi:10.1111/jam.14261

Cited by 8 publications

(3 citation statements)

References 88 publications

(206 reference statements)

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“…In addition to the natural environmental studies described in this review, microcosm/mesocosm experiments have been proposed to study the interactive effects of global change on organisms from different trophic levels, including microorganisms. This approach makes it possible to simulate the exposure of estuarine benthic communities under controlled laboratory conditions to directly associate changes with the source of environmental disturbance or stress (Coelho et al, 2015;Coelho et al, 2016;Louvado et al, 2019). Also, an emergent field of study is the evaluation of how bacterial interactions and their symbiotic relationships with higherlevel organisms are affected in response to environmental perturbations, mostly considering the vital importance of microbial communities in the hosts' development and health.…”

Section: Final Remarksmentioning

confidence: 99%

High‐throughput molecular analyses of microbiomes as a tool to monitor the wellbeing of aquatic environments

Michán

Blasco

Alhama

2021

Microbial Biotechnology

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Aquatic environments are the recipients of many sources of environmental stress that trigger both local and global changes. To evaluate the associated risks to organisms and ecosystems more sensitive and accurate strategies are required. The analysis of the microbiome is one of the most promising candidates for environmental diagnosis of aquatic systems. Culture-independent interconnected meta-omic approaches are being increasing used to fill the gaps that classical microbial approaches cannot resolve. Here, we provide a prospective view of the increasing application of these high-throughput molecular technologies to evaluate the structure and functional activity of microbial communities in response to changes and disturbances in the environment, mostly of anthropogenic origin. Some relevant topics are reviewed, such as: (i) the use of microorganisms for water quality assessment, highlighting the incidence of antimicrobial resistance as an increasingly serious threat to global public health; (ii) the crucial role of microorganisms and their complex relationships with the ongoing climate change, and other stress threats; (iii) the responses of the environmental microbiome to extreme pollution conditions, such as acid mine drainage or oil spills. Moreover, protists and viruses, due to their huge impacts on the structure of microbial communities, are emerging candidates for the assessment of aquatic environmental health.

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Section: Final Remarksmentioning

confidence: 99%

High‐throughput molecular analyses of microbiomes as a tool to monitor the wellbeing of aquatic environments

Michán

Blasco

Alhama

2021

Microbial Biotechnology

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“…Dispersants lower the interfacial tension between water and oil, and so reduce the energy required to convert a slick (or plume) into small oil droplets in the water column where they undergo rapid biodegradation (Prince, 2015b;Brandvik et al, 2019). However, there is currently a debate over whether dispersants have an effect on the biodegradation of crudeoil constituents (Kleindienst et al, 2015b(Kleindienst et al, , 2016Prince et al, 2016b), with conflicting reports in the literature showing it can be enhanced (Baelum et al, 2012;Techtmann et al, 2017;Tremblay et al, 2017), inhibited (Hamdan and Fulmer, 2011;Kleindienst et al, 2015a,b), or essentially unaffected (Prince et al, 2013;McFarlin et al, 2014;Louvado et al, 2019). In part this reflects a misunderstanding of the scale at which dispersants are designed to operate-they are formulated to encourage the dispersion of oil at sea that would otherwise remain as a floating slick, thereby dramatically increasing the surface area available for microbial colonization.…”

Section: Does the Use Of Dispersant Enhance Or Inhibit Mos Formation?mentioning

confidence: 99%

Marine Oil Snow, a Microbial Perspective

Gregson

McKew

Holland³

et al. 2021

Front. Mar. Sci.

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Under certain conditions, dispersed crude oil in the sea combines with organisms, organic matter, and minerals to form marine oil snow (MOS), thereby contributing to the sinking of oil to the seafloor. Marine microbes are the main players in MOS formation, particularly via the production of extracellular polymeric substances. Distinct groups of microbes also consume the majority of the hydrocarbons during descent, leading to enrichment of the less bioavailable hydrocarbons and asphaltenes in the residue. Here we discuss the dynamics of microbial communities in MOS together with their impacts on MOS evolution. We explore the effects of dispersant application on MOS formation, and consider ways in which laboratory experiments investigating MOS formation can be more representative of the situation in the marine environment, which in turn will improve our understanding of the contribution of MOS to the fate of spilled oil.

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“…The taxonomy results of species annotations are divided into 7 levels: kingdom (L1), phylum (L2), class (L3), order (L4), family (L5), genus (L6), and species (L7) for 16S rRNA amplicon. Additionally, the unassigned and singleton OTU were removed [33,34], and the effective Tag sequence number (No. of seqs) and OTU taxonomy comprehensive information table (OTU table) of each sample were obtained.…”

Section: S Rrna Gene Sequence Analysismentioning

confidence: 99%

A First Insight into the Structural and Functional Comparison of Environmental Microbiota in Freshwater Turtle Chinemys reevesii at Different Growth Stages under Pond and Greenhouse Cultivation

et al. 2020

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The microbial community structure of water is an important indicator for evaluating the water quality of the aquaculture environment. In this study, the investigation and comparison of the bacterial communities of pond cultivation (PC) and greenhouse cultivation (GC) between hatchling, juvenile, and adult growth stages of C. reevesii were performed. In addition, the V4 regions of the 16S rRNA gene were sequenced. The Chao1 richness estimator of the PC group was significantly higher than that of the GC group. The beta diversity showed that the microbiotas of the two groups were isolated from each other. The dominant phyla were Cyanobacteria, Proteobacteria, Actinobacteria, Bacteroidetes, Verrucomicrobia, and Planctomycetes in the PC group and Proteobacteria, Bacteroidetes, Firmicutes, Cyanobacteria, Chloroflexi, and Actinobacteria in the GC group. Both the numbers and the types of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotations differed between the PC and GC groups. The prediction of bacterial phenotype implied that the GC environment is more likely to deteriorate, and turtles are more susceptible to pathogens than those of the PC environment. In addition, a total of nine potential pathogenic bacteria were identified and the correlation of environmental factors analyses showed significant differences of bacterial species between the PC and GC groups, while the potential pathogenic bacteria showed significant correlation with the stocking density, temperature, pH, orthophosphate (PO4-P), and dissolved oxygen (DO) in both the PC and GC groups. Noticeably, this is the first report to describe the different microbiota characteristics of the different cultivation environments in the different growth stages of C. reevesii, which will provide valuable data for water quality adjustment, disease prevention, and the healthy breeding of turtles.

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Microcosm evaluation of the impact of oil contamination and chemical dispersant addition on bacterial communities and sediment remediation of an estuarine port environment

Cited by 8 publications

References 88 publications

High‐throughput molecular analyses of microbiomes as a tool to monitor the wellbeing of aquatic environments

High‐throughput molecular analyses of microbiomes as a tool to monitor the wellbeing of aquatic environments

Marine Oil Snow, a Microbial Perspective

A First Insight into the Structural and Functional Comparison of Environmental Microbiota in Freshwater Turtle Chinemys reevesii at Different Growth Stages under Pond and Greenhouse Cultivation

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