Microchip electrophoresis array-based aptasensor for multiplex antibiotic detection using functionalized magnetic beads and polymerase chain reaction amplification

Zhou, Lingying; Gan, Ning; Hu, Futao; Li, Tianhua; Cao, Yuting; Wu, Dazhen

doi:10.1016/j.snb.2018.02.136

Cited by 32 publications

(14 citation statements)

References 34 publications

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“…It should be noted that there are several relevant studies to our work that involve combinations of capillary electrophoresis and antibiotic study [22][23][24][25][26][27][28][29][30][31][32][33][34] . These studies are primarily larger channels while our presented work focuses on smaller channels and considerably shorter channel lengths.…”

Section: Microchip Capillary Electrophoresis Dairy Device Using Fluormentioning

confidence: 99%

Microchip capillary electrophoresis dairy device using fluorescence spectroscopy for detection of ciprofloxacin in milk samples

Bosma

Devasagayam

Singh

et al. 2020

Sci Rep

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Detecting antibiotics in the milk supply chain is crucial to protect humans from allergic reactions, as well as preventing the build-up of antibiotic resistance. The dairy industry has controls in place at processing facilities, but controls on dairy farms are limited to manual devices. Errors in the use of these manual devices can result in severe financial harm to the farms. This illustrates an urgent need for automated methods of detecting antibiotics on a dairy farm, to prevent the shipment of milk containing antibiotics. This work introduces the microchip capillary electrophoresis dairy device, a low-cost system that utilizes microchip capillary electrophoresis as well as fluorescence spectroscopy for the detection of ciprofloxacin contained in milk. The microchip capillary electrophoresis dairy device is operated under antibiotic-absent conditions, with ciprofloxacin not present in a milk sample, and antibiotic-present conditions, with ciprofloxacin present in a milk sample. The response curve for the microchip capillary electrophoresis dairy device is found through experimental operation with varied concentrations of ciprofloxacin. The sensitivity and limit of detection are quantified for the microchip capillary electrophoresis dairy device.

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Section: Microchip Capillary Electrophoresis Dairy Device Using Fluormentioning

confidence: 99%

Microchip capillary electrophoresis dairy device using fluorescence spectroscopy for detection of ciprofloxacin in milk samples

Bosma

Devasagayam

Singh

et al. 2020

Sci Rep

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“…Additionally, this assay evidenced to be a competent platform for multiplex antibiotic residue detection for the safety of food. This method can work on other antibiotic residue detection by exchanging the particular aptamer (Zhou et al, 2018). Chen and co-worker developed a multiplexed aptasensor based on the universal double-T-type microchip to one step for the simultaneous screening of numerous antibiotics in a very short period including kanamycin and chloramphenicol as a representative.…”

Section: Other Immunoassaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

119

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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“…Figure represents an example of the use of aptamer MCE for the detection of antibiotics in a food . A summary of the applications of aptamer MCE is listed in Table .…”

Section: Aptamer‐based Affinity Cementioning

confidence: 99%

Capillary electrophoresis–based immunoassay and aptamer assay: A review

2020

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Capillary electrophoresis-based immunoassay and aptamer assay: A reviewOver the last two decades, the group of techniques called affinity probe CE has been widely used for the detection and the determination of several types of biomolecules with high sensitivity. These techniques combine the low sample consumption and high separation power of CE with the selectivity of the probe to the target molecule. The assays can be defined according to the type of probe used: CE immunoassays, with an antibody as the probe, or aptamer-based CE, with an aptamer as the probe. Immunoassays are generally divided into homogeneous and heterogeneous groups, and homogeneous variant can be further performed in competitive or noncompetitive formats. Interacting partners are free in solution at homogeneous assay, as opposed to heterogeneous analyses, where one of them is immobilized onto a solid support. Highly sensitive fluorescence, chemiluminescence or electrochemical detections were typically used in this type of study. The use of the aptamers as probes has several advantages over antibodies such as shorter generation time, higher thermal stability, lower price, and lower variability. The aptamer-based CE technique was in practice utilized for the determination of proteins in biological fluids and environmentally or clinically important small molecules. Both techniques were also transferred to microchip. This review is focused on theoretical principles of these techniques and a summary of their applications in research.Abbreviations: Ag, antigen; CEIA, CE immunoassay; MCE, microchip CE; SELEX, systematic evolution of ligands by exponential enrichment and they are produced by the immune system of living organisms. Ag that start the immunological response are also called immunogens. These Ab-Ag complexes are stabilized by dipole-dipole, van der Waals or hydrophobic interactions and hydrogen bonds [5,6].The most often used immunoassays are ELISA with Ab or Ag immobilized onto solid support (plates, glass fibers, or plastic tubes), immunofluorescence assay, fluorescence polarization immunoassay, luminescence immunoassay, microparticle enzyme immunoassay, and biosensors. The major disadvantages of immunoassays are the tedious process, time-consuming reactions, and problems with reproducibility and nonspecific interactions [6].CE is a powerful analytical method for the separation of a wide range of molecules. The coupling of CE and immunoassay in the new CE immunoassay (CEIA) technique provided both separation power and specificity of interactions. Other advantages are the possibility of multianalyte analyses, automation, low sample consumption, high speed, flexibility, a lower amount of false-positive results, and also the possibility of connection with highly sensitive detectors [5].In CEIA, the Fab fragments of Ab containing Ag-specific regions are more often used than the whole monoclonal and Color online: See article online to view Figs. 4 and 7 in color.

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Microchip electrophoresis array-based aptasensor for multiplex antibiotic detection using functionalized magnetic beads and polymerase chain reaction amplification

Cited by 32 publications

References 34 publications

Microchip capillary electrophoresis dairy device using fluorescence spectroscopy for detection of ciprofloxacin in milk samples

Microchip capillary electrophoresis dairy device using fluorescence spectroscopy for detection of ciprofloxacin in milk samples

Current advances in immunoassays for the detection of antibiotics residues: a review

Capillary electrophoresis–based immunoassay and aptamer assay: A review

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