Microchip capillary electrophoresis using on-line chemiluminescence detection

Hashimoto, Masahiko; Tsukagoshi, Kazuhiko; Nakajima, Riichiro; Kondo, Kazuo; Arai, Akihiro

doi:10.1016/s0021-9673(99)01169-3

Cited by 105 publications

(85 citation statements)

References 23 publications

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“…In addition, the CL reagent was delivered by electroosmotic flow, as a result, some CL systems could not be employed when CL reagent was only dissolved in organic solvent. The end-channel batch-type detection cell for CL detection on microchip was also demonstrated by Hashimoto et al [23] Dansyl amino acids were monitored using a peroxyoxalate CL system. Since the detection cell also worked as the waste reservior, the waste produced by the CL reaction was accumulated in the detection cell, which would have a negative influence on the sensitivity and reproducibility.…”

Section: Introductionmentioning

confidence: 95%

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Lin

Uchiyama

et al. 2004

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Section: Introductionmentioning

confidence: 95%

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Lin

Uchiyama

et al. 2004

Talanta

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“…[5][6][7][8][9] Recently, CL detection is also estimated to be one of the most matched detection methods to the micro total analysis system (µ-TAS), because CL does not require any light source or spectroscopes. 10,11 The CL from synthetic compounds, including luminol, peroxyoxalate ester, ruthenium complex, lophine derivative, acridinium ester, and adamantyldioxetane compound, have been known for a long time, and many analytical applications of them have been reported. [12][13][14] The CL emitted by luminol is remarkably enhanced by a catalyst, such as metal ions.…”

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confidence: 99%

Development of FIA Equipped with a Chemiluminescence Detector Using a Mixed Reagent of Luminol and 1,10-Phenanthroline

2003

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Absorbance and fluorescence phenomena have been the most basic principles for the detection technique in an instrumental analysis. Also, chemiluminescence (CL), which has a profound relationship to their detection principles, has received much attention as an attractive detection technique. CL detection has the followings characteristics: highly sensitive, determinable over a wide range, easy to operate, and inexpensive apparatus and reagent. It has been used in FIA, HPLC, 1-4 and capillary electrophoresis. [5][6][7][8][9] Recently, CL detection is also estimated to be one of the most matched detection methods to the micro total analysis system (µ-TAS), because CL does not require any light source or spectroscopes. 10,11 The CL from synthetic compounds, including luminol, peroxyoxalate ester, ruthenium complex, lophine derivative, acridinium ester, and adamantyldioxetane compound, have been known for a long time, and many analytical applications of them have been reported. [12][13][14] The CL emitted by luminol is remarkably enhanced by a catalyst, such as metal ions. 15 This has been used for the determination of small amounts of metal ions and their complexes. The reaction of peroxyoxalate reagents, such as bis(2,4,6-trichlorophenyl)oxalate (TCPO) and H2O2, induces CL through energy transport to co-existing fluorescence compounds. 16 The peroxyoxalate CL reaction, which can detect fluorescence-labeled compounds, is useful and attractive in CL analyses, because the labeling for fluorescence methods has been widely investigated and the technique can be easily applied for CL detection using peroxyoxalate reagents.Tris(2,2′-bipyridine)ruthenium(II) ion (Ru(bpy)3 2+ ) has also been utilized as a CL reagent, where Ru(bpy)3 3+ , which is obtained electrochemically, oxidizes various organic amines. 17 The oxidant, Ru(bpy)3 3+, generally reacts best with tertiary, next with secondary, and with primary alkyl amines. The CL reaction was utilized to determine antibiotic compounds, like erythromycin and clindomycin, which both possess reactive tertiary amine groups. 18,19 However, compared to absorption and fluorescence detections, it is possible that CL detection lacks generality in its use. In order to solve this problem, many scientists have tried to synthesize novel CL reagents and to explore more effective analytical conditions. 2 New reaction phases or interfaces to change the CL property also been introduced into the CL reaction for a trace analysis of metal ions, H2O2, dyestuffs, etc. 20,21The local microenvironment in ordered surfactant assemblies, such as micelles, reversed micelles, and bilayer membranes, resulted in significant improvements in the analytical performance of CL reaction systems. For example, the CL mechanism in 1,10-phenanthroline (phen) oxidation during the catalytic decomposition of H2O2 was reported in 1982 by Fedorova et al., 22 and Ishii et al. found out micellar enhanced CL using phen-H2O2-cetyltrimethylammonium bromide (CTAB) system to determine Cu(II) at the sub-picogram level. 23 We stud...

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“…[6][7][8] Recently, the CL detection is also estimated to be one of the detection methods best matched to the micro total analysis system (µ-TAS), because CL does not require any light source or spectroscopes. 9,10 The CL detection features high sensitivity, wide determinable range, easy operation, inexpensive apparatus and reagent, and simple construction in an instrument.…”

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confidence: 99%

Development of Ultra-micro Flow Analysis with Chemiluminescence Detector

2003

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We proposed to combine an ultra-micro flow analysis instrument using the fused-silica capillary and the CL detector. The CL reaction of luminol and hydrogen peroxide was adopted and the batch-type CL detection cell was used. Luminol and isoluminol-labeled protein as a model were sensitively and reproducibly detected with very small amounts of quantitative reagents. The analyses were repeated at least 100 times without any treatments such as washing capillaries or exchanging the hydrogen peroxide solution. The present system successfully promoted the miniaturization, simplification, and sensitization of the analytical system.

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Microchip capillary electrophoresis using on-line chemiluminescence detection

Cited by 105 publications

References 23 publications

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Integration of a flow-type chemiluminescence detector on a glass electrophoresis chip

Development of FIA Equipped with a Chemiluminescence Detector Using a Mixed Reagent of Luminol and 1,10-Phenanthroline

Development of Ultra-micro Flow Analysis with Chemiluminescence Detector

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