Microchannel liquid-flow focusing and cryo-polymerization preparation of supermacroporous cryogel beads for bioseparation

Yun, Junxian; Tu, Changming; Lin, Dong‐Qiang; Xu, Linzhi; Guo, Yantao; Shen, Shihua; Zhang, Songhong; Yao, Kangde; Guan, Yong; Yao, Shan‐Jing

doi:10.1016/j.chroma.2012.05.075

Cited by 41 publications

(17 citation statements)

References 69 publications

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“…In recently published papers, the characterisation of solid composite cryogels with silica [228], carbon [229,230], carbon/carbon [231] or carbon/silica composite [92] Yun et al [247] synthesised polyacrylamide based supermacroporous cryogel beads with a narrow particle size distribution at average particle size around 1 mm with high porosity of 84-98%.…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

279

212

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

279

212

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“…The reaction proceeding in this nonfrozen microzone, with the ice crystals as the porogen, could generate monolithic matrices with interconnected pores, ranging from 10 to 100 mm in size after thawing. The macropore size and the hydrophilicity of monolithic polyacrylamide cryogel made it possible for drug targets to be screened against large biomolecules such as phages and cells (Yun et al, 2012;Önnby et al, 2012;Billakanti and Fee, 2009;Kumar et al, 2006;Plieva et al, 2006;Ahlqvist et al, 2006) to transfer without resistance. Another advantage of monolithic cryogel was the introduction of epoxy moiety by AGE, which was convenient for the subsequent doxorubicin immobilization.…”

Section: Resultsmentioning

confidence: 99%

“…The polyacrylamide cryogel is a kind of monolithic material prepared from the monomer acrylamide, ally glycidyl ether (AGE), and the cross-linker methylene-bisacrylamide. The macroporous nature and the hydrophilicity of the cryogel make it possible for large bioparticles to pass the gel unhindered, and so it is commonly used for affinity absorption of metal ions, purification of proteins and separation of inclusion bodies, viruses, Escherichia coli and mammalian cells (Yun et al, 2012;Önnby et al, 2012;Billakanti and Fee, 2009;Kumar et al, 2006;Plieva et al, 2006;Ahlqvist et al, 2006;Williams et al, 2005). The introduction of an epoxy group by AGE makes it easy to couple ligand molecules on the surface of the cryogel by the present available chemical method.…”

Section: Introductionmentioning

confidence: 99%

Screening of phage‐displayed human liver cDNA library against doxorubicin with drug‐immobilized monolithic polyacrylamide cryogel

Yu¹,

Zhao

Zhang

et al. 2013

Biomedical Chromatography

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Monolithic polyacrylamide cryogel was prepared and utilized as a new matrix for drug immobilization to screen against phage-displayed human liver cDNA library. The macropores and hydrophilic nature of the cryogel made it possible for phage particles to pass unhindered. Doxorubicin, an anticancer drug, was covalently bonded to the monolithic cryogel by the glutaraldehyde method, and after five rounds of affinity selection performed in an SPE cartridge, phage clones that displayed Homo sapiens methyl CpG binding protein 2 (MeCP2 ) were selectively enriched. The interaction between doxorubicin and MeCP2 displayed phages was further validated by studying the retention of doxorubicin on MeCP2 phage-coupled cryogel. These results demonstrate that drug-coupled polyacrylamide cryogel might be a promising kind of matrix for screening target proteins against phage-displayed library.

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“…Polyacrylamide (pAAm)‐based cryogels are a popular class of supermacroporous monoliths in biotechnology applications. It is also possible to prepare crygels in bead form with similar methods, i.e., by freezing‐thawing aqueous droplets containing gel‐forming monomers . Most of these methods are convenient to operate and suitable for preparing cryogel beads used for immobilization purposes, but they also suffer from challenges such as accurately controlling of bead sizes and need relatively complex equipments.…”

Section: Introductionmentioning

confidence: 99%

“…In this work, we used water crystallizes as porogens and applied a traditional inverse suspension polymerization technique at subzero temperature. A new method to prepare pAAm‐based cryogel beads was also proposed . A direct compounding method was used for the fabrication of a pAAm‐based composite cryogel beads with nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of supermacroporous polyacrylamide cryogel beads for biotechnological application

Xiaoyong

Lin

et al. 2013

J of Applied Polymer Sci

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Polymer beads, particularly supermacroporous beads, play important roles in biotechnological applications, such as their application as adsorbents in bioseparation processes and used as carriers in immobilization of cells or/and enzymes. In this study, supermacroporous polyacrylamide (pAAm) based cryogel beads were prepared via an inverse suspension polymerization method at low temperature (<0 C). Standard porous beads were also prepared at room temperature for comparison. The results showed that the diameter of the beads were in the range of 50-400 lm and some irregular large pores of the beads were of 3-90 lm in diameter, which had good biocompatibility, hydrophilicity, high porosity and large connected pores. To modify the properties of these cryogel beads, cryogel beads embedded with nanoparticles of TiO 2 were also prepared. The physical properties of the composite cryogel beads with supermacroporous were characterized and the results showed an improvement in mechanical strength and stability, which indicated that these supermacroporous cryogel beads may be useful in biotechnological applications as the networks in the beads can facilitate the mass transfer of nutrients and oxygen.

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Microchannel liquid-flow focusing and cryo-polymerization preparation of supermacroporous cryogel beads for bioseparation

Cited by 41 publications

References 69 publications

Cryogels: Morphological, structural and adsorption characterisation

Cryogels: Morphological, structural and adsorption characterisation

Screening of phage‐displayed human liver cDNA library against doxorubicin with drug‐immobilized monolithic polyacrylamide cryogel

Preparation and characterization of supermacroporous polyacrylamide cryogel beads for biotechnological application

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