Microbodies in fungi: a review

Carson, David B.; Cooney, J. J.

doi:10.1007/bf01576172

Cited by 10 publications

(3 citation statements)

References 125 publications

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“…In the processes of stoichiometry for cofactors as well as the information on reversibility or irreversibility for each reaction, these were verified and added as information into the reconstructed network. Different cellular compartments were considered and consequently biochemical reactions were distributed into four different compartments: the extracellular space, the cytosol, the mitochondria, and the peroxisome [44]. Identification of localization of each biochemical reaction was analyzed according to enzyme localization, which was performed by applying protein localization predictors.…”

Section: Methodsmentioning

confidence: 99%

Improved annotation through genome-scale metabolic modeling of Aspergillus oryzae

et al. 2008

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Background: Since ancient times the filamentous fungus Aspergillus oryzae has been used in the fermentation industry for the production of fermented sauces and the production of industrial enzymes. Recently, the genome sequence of A. oryzae with 12,074 annotated genes was released but the number of hypothetical proteins accounted for more than 50% of the annotated genes. Considering the industrial importance of this fungus, it is therefore valuable to improve the annotation and further integrate genomic information with biochemical and physiological information available for this microorganism and other related fungi. Here we proposed the gene prediction by construction of an A. oryzae Expressed Sequence Tag (EST) library, sequencing and assembly. We enhanced the function assignment by our developed annotation strategy. The resulting better annotation was used to reconstruct the metabolic network leading to a genome scale metabolic model of A. oryzae.

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Section: Methodsmentioning

confidence: 99%

Improved annotation through genome-scale metabolic modeling of Aspergillus oryzae

et al. 2008

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“…As stated by Carson and Cooney (1990) isolation of microbodies from filamentous fungi is difficult because the organelles are fragile and they are enclosed within a tough cell wall. This is probably also the case for similar singlemembrane-bound organelles such as Woronin bodies.…”

Section: Separation Of Cell Components By Differential Centrifugationmentioning

confidence: 99%

Immunocytochemical Localization of Phytotoxins inBipolaris sorokiniana

Åkesson

Carlemalm

Everitt

et al. 1996

Fungal Genetics and Biology

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“…Since adipate is costly compared to glucose, adipate degradation is undesirable. At present it is, however, unknown where and how the degradation of adipate takes place, and what the degradation products are, but there is reason to believe that degradation of adipate takes place in the microbodies in a process similar to that of b-oxidation of fatty acids (Baltazar et al, 1999;Carson and Cooney, 1990;Valenciano et al, 1996).…”

Section: Introductionmentioning

confidence: 96%