Microbiotests in aquatic toxicology: the way forward

Abstract

The industrial revolution has driven the need for ecotoxicology and shaped its evolution. Indeed, the increased use and transformation of (non)renewable resources for over a century to benefit mankind have had a downside and created a plethora of contaminants harmful to the receiving environments. With time, we have gone from an age of darkness in the 1950s (i.e., diagnostic ignorance in terms of recognizing and dealing with contamination) to one of enlightenment as the 21 st century unfolds (i.e., use of tools and strategies to identify and correct environmental pollutions). Effects measurements, reflected by toxicity testing conducted at different levels of biological organization, have proven especially useful to achieve proper hazard/risk assessments of contaminants. Knowing why toxicity testing has been conducted over the past decades to protect and conserve freshwater environments is also essential to grasp the importance and breadth of this field. For this purpose, we have recently reviewed a substantial number of articles describing numerous bioanalytical endeavours undertaken to comprehend toxic effects associated with the discharge of xenobiotics to aquatic environments. Scrutiny of publications identified in our literature search has enabled us to uncover the various ways in which laboratory toxicity tests have been applied, many of which are small-scale in nature. In essence, freshwater toxicity testing has significantly focussed on liquid (complex environmental samples, chemical and biological contaminants) and solid media (sediments) assessment. For both media, miscellaneous studies/initiatives linked to toxicity testing applications have again promoted the development, validation, refinement and use of toxicity testing procedures. Bioassays are clearly an essential component of environmental management programs and several small-scale tests (microbiotests) can be employed to generate cost-effective toxicity data that assist decision-making.

Ecotoxicological biotests as tools for continuous monitoring of water quality in dam reservoir

“…Small invertebrates, such as crustaceans, protozoans, algae, yeasts, plant seedlings, bacteria, shellfish, fish and amphibians, are used as the test organisms (Namiesnik and Szefer 2010). Numerous researchers have promoted the usage of biotests in Europe (Blaise and Férard 2006, Wadhia and Dando 2009, Palma et al 2010, Wolska et al 2010. The advantages of biotests are their broad range sensitivity, technical simplicity, low costs and short time necessary for assay completion (Namiesnik and Szefer 2010).…”

Sewage sludge hazard index based on bioassays: strategic tool for the decision-making process on sludge agricultural use

“…In 1993, Environment Canada developed the Potential Ecotoxic Effects Probe (PEEP), a scientifically management tool, based on ecotoxicological principles, simple to use and interpret, with a good discriminatory potential to assess wastewater toxic loads. It was applied to prioritize corrective or preventive actions regarding point source emissions in Saint-Lawrence River Canada (Blaise and Férard, 2005;Costan et al, 1993).…”