Microbiota of catfish (Clarias gariepinus) tissues harvested from vials polluted with soil from e-wastes dumpsite

Abstract

In this study the microbiota of Clarias gariepinus tissues harvested from e-waste-soil polluted vials were assessed. Soil samples contaminated with e-waste were analyzed using standard analytical protocol while the microbial study was obtained using standard conventional microbiological techniques. The results revealed that the soil was sandy-loamy and blackish in colour. High organic matter (17.60%) and organic carbon (10.17%) were obtained. Also, higher calcium (182.00 mg/kg) and phosphorus (146.65 mg/kg) contents compared to other mineral constituents were recorded while, the heavy metals ranged from 0.32-64.90 mg/kg. Bacterial count ranged from 9.0 × 102 to 4.0 × 103 cfu/L while the fungal count from 4.0 × 102 to 2.3 ×103 sfu/L. The genera of bacteria isolate were identified as Staphylococcus, Proteus, Bacillus, Listeria, Salmonella, Enterobacter, Pseudomonas, Lactobacillus and Corynebacterium and fungal isolates were Penicilliun, Candida, Articulospora, Aspergillus, Rhizopus, Mucor, Zoopage, Varcosporium and Rhodotorula. Microbial species richness of fish tissues from polluted vials indicates a more poly-diverse microbial community compared to those from unpolluted vial. Differences were observed in the occurrence of fungi on the surfaces of catfish tissues in control vial compare to those on fishes from polluted vials. Hence, there is a need for proper water management for fish culturing and caution in the exploration of fishes from polluted natural waters for human consumption.

Microbial Flora of Smoke-Dried Catfish (Clarias Gariepinus) Processed and Sold in Some Abattoirs in Bayelsa and Rivers States

“…The organisms obtained in this investigation have been reported in smoked fish by other researchers. The organisms obtained in this investigation have been reported in catfish by other researchers (Adegunloye and Sanusi, 2019;Daramola et al, 2020). This report corroborates the report of Oku and Amakoromo (2013) who reported the presence of Bacillus and Pseudomonas sp.…”

Bacteriological Assessment and Antibiotic Susceptibility Profile of Bacteria Recovered from <i>Clarias gariepinus</i> Selected from Various Fish Farms in Anambra North Senatorial Zones in Anambra State, Nigeria

“…They recorded counts ranging from 0.73 ± 0.0030 × 10 3 -2.55 ± 0.0128 × 10 3 cfu/g. Similar range of bacterial count was recorded by [5], during their work on microbiota of catfish (Clarias gariepinus) tissues, and opined that the higher microbial load in gills than liver and intestine is due to the gill's constant interaction with the water environment. On the contrary, very high bacterial load of 12.10 ± 0.42 × 10 12 CFU/g in the intestine, followed by gill (12.10 ± 0.42 × 10 12 CFU/g) and least in the liver (6.48 ± 1.06 × 10 6 CFU/g) of Tilapia was reported by [26], during their work on prevalence and antimicrobial susceptibility of pathogenic bacteria in Nile Tilapia, Oreochromis niloticus L. They also noted that poor sanitary conditions could have been the cause of such high load.…”

“…Clarias gariepinus is freshwater fish species of African origin and is one of the most cultured species in the world. This fish species is valuable for fish farming (aquaculture) due to its easy to farm especially in warm climates, fast growth rate, resistance to diseases, and high stocking density [5]. Fishes are reared in different water culture media or confinement such as concrete, earthen or plastic ponds.…”