Microbiota Diversity in Human Colorectal Cancer Tissues Is Associated with Clinicopathological Features

Wu, Yaxin; Shi, Lei; Li, Qing; Wu, Jiao; Peng, Wei; Li, Huan; Chen, Kequan; Ren, Yixing; Fu, Xiangsheng

doi:10.1080/01635581.2019.1578394

Cited by 23 publications

(22 citation statements)

References 38 publications

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“…A study of F. nucleatum's DNA presence in formalinxed para n-embedded tumour slides showed that the proportion of this species increased with tumour histological grade [88]. Wu et al [36] report 15 genera associated with tumour mucosa of moderately and poorly differentiated tumours compared to well differentiated tumours. In our study, the sample size allowed the inclusion of an interaction term, thus providing a ner estimation of differences in microbiome composition with respect to tumour grade.…”

Section: Tumour Crc Microbial Subtypesmentioning

confidence: 98%

“…given the spatial variation in microbiome's taxonomic structure in different parts of gut [36], the effect of tumour localisation on grade-microbiota interaction should be estimated. So far, few studies have associated microbiome of CRC patients with the tumour grade using different approaches (real-time PCR, 16S rRNA sequencing) but without considering the interactions between tumour sidedness and grade.…”

Section: Tumour Crc Microbial Subtypesmentioning

confidence: 99%

“…Liu et al [30] showed that the bacterial communities in both tumour tissue and visually-normal tissue were similar, but the study was largely underpowered (N = 8 individuals) and did not answer the question of the clinical relevance of this similarity. Other studies associated microbiome on tumour or in stool with clinical variables [31,35,36] but had a similar disadvantage in terms of statistical power (N = 25, N = 30, N = 53, individuals, respectively).…”

Section: Introductionmentioning

confidence: 99%

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Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Zwinsová¹,

Петров²,

Hrivňáková³

et al. 2020

Preprint

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BackgroundLong-term dysbiosis of the gut microbiome has a significant impact on the development, progression and the aggressiveness of colorectal cancer (CRC) and may explain part of the observed heterogeneity of the disease from phenotypic, prognostic and response to treatment perspectives. Although the shifts in gut microbiome in the normal-adenoma-carcinoma sequence have been described, the landscape of microbiome within CRC and its associations with clinical variables remain under-explored. ResultsWe performed 16S rRNA gene sequencing of paired tumour tissue, adjacent visually-normal mucosa and stool swabs of N=186 patients with stage 0-IV CRC to describe the tumour microbiome and its association with clinical variable and to derive tumour microbial subtypes.We identified new genera never previously associated with CRC tumour mucosa (Flavonifractor, Haemophilus, Howardella, Pseudomonas, Sutterella, Treponema 2) or CRC (Actinobacillus, Aggregatibacter, Bergeyella, Phocaeiola, Defluviitaleaceae UCG-011, Massilia, Tyzzerella 4). The bacteria residing on tumour-mucosa were dominated by genera belonging to (potential) oral pathogens. Based on tumour microbial profiles, we stratified CRC patients into three subtypes. The subtypes were significantly associated with prognostic factors such as tumor grade, primary tumour sidedness and TNM staging, with one subtype enriched in tumours with poor prognosis. Further, we inspected the associations of microbiome with clinical variables in a subtype-agnostic setting. The primary tumour-associated clinical variables predominantly correlated with tumour mucosal microbiome, while the presence of local and distant metastases was mostly associated with the stool microbiome.ConclusionsUnderstanding the interactions of the bacteria residing on tumour mucosa within different CRC tumour microbiome subtypes will help to better understand the underlying biological background of the heterogeneity of this disease. Indeed, the tumour microbiome is a possible source of additional integrative markers of CRC patients’ survival and prognosis. We found that CRC microbiome is strongly correlated with clinical variables, but these associations are dependent on the microbial environment (tumour mucosa, normal mucosa, stool). Our study thus identifies limitations of the usage of microbiome composition as marker of CRC progression, suggesting the need of combining several sampling sites (e.g. stool and tumour swabs).

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Section: Tumour Crc Microbial Subtypesmentioning

confidence: 98%

Section: Tumour Crc Microbial Subtypesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Zwinsová¹,

Петров²,

Hrivňáková³

et al. 2020

Preprint

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“…5 In contrast, H. pylori infection was shown to reduce the risk of IBD. 6 In another study, PCR-based investigation failed to show any correlation between enterohepatic Helicobacter species (H. bilis, Helicobacter hepaticus and Helicobacter pullorum) and cholelithiasis, while the presence of H. pylori DNA was associated with acute gallstone cholecystitis. Analysis of microbiota in human colorectal cancer (CRC) tissues revealed that the number of Helicobacter species in patients with poorly differentiated right colon cancers was higher than in well-differentiated left colon cancers.…”

mentioning

confidence: 96%

“…In addition, Helicobacter bilis was overrepresented in poorly differentiated cancers as compared to well-differentiated cancers, suggesting that H. bilis is potentially associated with a poorer differentiation and more negative prognosis in patients with CRC. 6 In another study, PCR-based investigation failed to show any correlation between enterohepatic Helicobacter species (H. bilis, Helicobacter hepaticus and Helicobacter pullorum) and cholelithiasis, while the presence of H. pylori DNA was associated with acute gallstone cholecystitis. 7 Using NGS-based 16S rRNA sequencing, Helicobacter species were not detected either in bile collected from Chilean patients with gallbladder cancer, 8 contradicting previous studies.…”

mentioning

confidence: 96%

Review: Other Helicobacter species

Ménard

Smet

2019

Helicobacter

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This article is a review of the most important, accessible, and relevant literature published between April 2018 and April 2019 in the field of Helicobacter species other than Helicobacter pylori. The initial part of the review covers new insights regarding the presence of gastric and enterohepatic non‐H. pylori Helicobacter species (NHPH) in humans and animals, while the subsequent section focuses on the progress in our understanding of the pathogenicity and evolution of these species. Over the last year, relatively few cases of gastric NHPH infections in humans were published, with most NHPH infections being attributed to enterohepatic Helicobacters. A novel species, designated “Helicobacter caesarodunensis,” was isolated from the blood of a febrile patient and numerous cases of human Helicobacter cinaedi infections underlined this species as a true emerging pathogen. With regard to NHPH in animals, canine/feline gastric NHPH cause little or no harm in their natural host; however they can become opportunistic when translocated to the hepatobiliary tract. The role of enterohepatic Helicobacter species in colorectal tumors in pets has also been highlighted. Several studies in rodent models have further elucidated the mechanisms underlying the development of NHPH‐related disease, and the extra‐gastric effects of a Helicobacter suis infection on brain homeostasis was also studied. Comparative genomics facilitated a breakthrough in the evolutionary history of Helicobacter in general and NHPH in particular. Investigation of the genome of Helicobacter apodemus revealed particular traits with regard to its virulence factors. A range of compounds including mulberries, dietary fiber, ginseng, and avian eggs which target the gut microbiota have also been shown to affect Helicobacter growth, with a potential therapeutic utilization and increase in survival.

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Gut Microbiome in Health and Gastrointestinal Cancer

Lau

2023

Microbiome in Gastrointestinal Cancer

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Microbiota Diversity in Human Colorectal Cancer Tissues Is Associated with Clinicopathological Features

Cited by 23 publications

References 38 publications

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Tumour Microbiome-Based Subtypes of Colorectal Cancer Correlate with Clinical Variables

Review: Other Helicobacter species

Gut Microbiome in Health and Gastrointestinal Cancer

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