Microbiosensor for the detection of acetate in electrode-respiring biofilms

Atçı, Erhan; Babauta, Jerome T.; Sultana, Sujala T.; Beyenal, Haluk

doi:10.1016/j.bios.2016.03.027

Cited by 47 publications

(18 citation statements)

References 45 publications

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“…3 ). We can rule out mass transport limitations because the biofilms investigated in this study were too thin (<50 μm) to restrict the diffusion of the electron donor, acetate 38 . Furthermore, the phenotypes of the pili-deficient pilB mutant in MECs were similar to those of a Tyr3 mutant, which produces pili with reduced conductivity ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Mechanistic stratification in electroactive biofilms of Geobacter sulfurreducens mediated by pilus nanowires

2016

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Electricity generation by Geobacter sulfurreducens biofilms grown on electrodes involves matrix-associated electron carriers, such as c-type cytochromes. Yet, the contribution of the biofilm's conductive pili remains uncertain, largely because pili-defective mutants also have cytochrome defects. Here we report that a pili-deficient mutant carrying an inactivating mutation in the pilus assembly motor PilB has no measurable defects in cytochrome expression, yet forms anode biofilms with reduced electroactivity and is unable to grow beyond a threshold distance (∼10 μm) from the underlying electrode. The defects are similar to those of a Tyr3 mutant, which produces poorly conductive pili. The results support a model in which the conductive pili permeate the biofilms to wire the cells to the conductive biofilm matrix and the underlying electrode, operating coordinately with cytochromes until the biofilm reaches a threshold thickness that limits the efficiency of the cytochrome pathway but not the functioning of the conductive pili network.

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Section: Discussionmentioning

confidence: 99%

Mechanistic stratification in electroactive biofilms of Geobacter sulfurreducens mediated by pilus nanowires

2016

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“…Based on an archetype, microbial fuel cell (MFC), multiple biosensors were proposed for water quality measurement with this concept (e.g., biochemical oxygen demand and toxic components; Abrevaya et al, 2015a,b; ElMekawy et al, 2018). Among which, one promising and important application is the detection of volatile fatty acids (VFAs) during anaerobic digestion (AD) or fermentation processes (Atci et al, 2016; Jin et al, 2017; Kretzschmar et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

The Potential of Bioelectrochemical Sensor for Monitoring of Acetate During Anaerobic Digestion: Focusing on Novel Reactor Design

Sun

Angelidaki

et al. 2019

Front. Microbiol.

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Acetate as the dominant fraction of volatile fatty acids (VFAs) is an important intermediate in metabolic pathways of methanogenesis, which could reflect the stability status of anaerobic digestion (AD) process. Bioelectrochemical sensors for environmental or bioprocess monitoring have become increasingly attractive in recent years. Although it was more favorable, several challenges still need to be addressed for acetate detection, including large electrode spacing, low stability, biofouling at the cathode and low detection range. In this study, an innovative biosensor on the basis of a three-chamber microbial electrochemical system was proposed to monitor the acetate during the AD process. In such a system, acetate was first transferred from sample chamber through the anion exchange membrane (AEM) to anode due to the driven force of concentration difference and then oxidized by anodic biofilm as a substrate for the current generation. With such design, the influence of waste properties fluctuation in the cathodic reaction could be avoided. The response of current density to different acetate concentrations was investigated. The selectivity, the influence of the sample temperature and the external resistance were also evaluated. The correlation (R2 > 0.99) between the current densities and acetate concentrations (up to 160 mM) was established at specific reaction time (from 2 to 5 h). Current densities after 5 h reaction were improving about 20% when the sample temperature was high (e.g., 37 and 55°C). The detection range increased along with the decrease of external resistance. The acetate concentrations of AD effluents as determined by the biosensor where within 24.2% of the ones determined by gas chromatography. Nevertheless, the application of the biosensor for monitoring acetate in environmental samples could still be promising.

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“…The process of biofilm formation is of high importance in many industrial applications such as the biodegradation of chemical contaminants in wastewater, biocatalysis, and microbial fuel cells [ 18 , 60 , 61 ]. However, other biofilms may have serious implications on public health and environment [ 62 ].…”

Section: Bioelectrochemistry Of Biofilmsmentioning

confidence: 99%

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

Hassan

Febbraio

Andreescu

2021

Sensors

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Microbial electrochemical systems are a fast emerging technology that use microorganisms to harvest the chemical energy from bioorganic materials to produce electrical power. Due to their flexibility and the wide variety of materials that can be used as a source, these devices show promise for applications in many fields including energy, environment and sensing. Microbial electrochemical systems rely on the integration of microbial cells, bioelectrochemistry, material science and electrochemical technologies to achieve effective conversion of the chemical energy stored in organic materials into electrical power. Therefore, the interaction between microorganisms and electrodes and their operation at physiological important potentials are critical for their development. This article provides an overview of the principles and applications of microbial electrochemical systems, their development status and potential for implementation in the biosensing field. It also provides a discussion of the recent developments in the selection of electrode materials to improve electron transfer using nanomaterials along with challenges for achieving practical implementation, and examples of applications in the biosensing field.

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Microbiosensor for the detection of acetate in electrode-respiring biofilms

Cited by 47 publications

References 45 publications

Mechanistic stratification in electroactive biofilms of Geobacter sulfurreducens mediated by pilus nanowires

Mechanistic stratification in electroactive biofilms of Geobacter sulfurreducens mediated by pilus nanowires

The Potential of Bioelectrochemical Sensor for Monitoring of Acetate During Anaerobic Digestion: Focusing on Novel Reactor Design

Microbial Electrochemical Systems: Principles, Construction and Biosensing Applications

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