Microbiomes From Biorepositories? 16S rRNA Bacterial Amplicon Sequencing of Archived and Contemporary Intestinal Samples of Wild Mammals (Eulipotyphla: Soricidae)

Abstract

Microbiomes From Biorepositories samples also influenced bacterial community composition. Additionally, we compare results of OTU based and ASV based analyses. Looking ahead, field collectors and museums should develop and adopt best practices related to frozen preservation to ensure adequate material for future microbiome investigations.

Opportunities and challenges for high‐quality biodiversity tissue archives in the age of long‐read sequencing

“…Improving the efficiency of sampling and storage protocols will substantially benefit field biologists and NHCs, and allow them to preserve a greater diversity of biomolecules (e.g., RNA, proteins, metabolites) with the same financial resources. Historical specimens and their associated tissues are now used to study many different biological topics; ranging from microbiomes (Greiman et al, 2020), epigenetic change (Hahn et al, 2020) to pathogen discovery (Cook et al, 2020). However, it is important to recognize that preservation approaches which work well for one purpose (e.g., DNA preservation), may actually prevent the study of other aspects (e.g., external morphology; Marquina et al, 2021).…”