Cichlids of the tribe Heroini have long been a source of taxonomical conflict. In particular, the species included in the Herichthys bartoni group have failed to be recovered as monophyletic in different molecular studies. In this paper we use traditional and geometric morphometrics to evaluate morphological variation in the species included in the H. bartoni complex in order to evaluate the number of species it contains. An update of a previously published DNA barcoding study suggests the existence of three genetic clusters that included the six recognized species analyzed in this study, none of them recovered as monophyletic. On the other hand, geometric morphometrics arise as a useful tool to discriminate species due that traditional morphometrics showed a high overlap in the characters analyzed that prevents the proposal of diagnostic characters.Los cíclidos de la tribu Heroini han experimentado un largo conflicto taxonómico. En particular, las especies incluidas en el grupo Herichthys bartoni no han sido recuperadas como monofiléticas en diversos estudios moleculares. En este artículo nosotros usamos morfometría tradicional y geométrica para evaluar la variación morfológica de las especies incluidas en el complejo H. bartoni para evaluar el numero de especies que contiene. Una actualización de un estudio previo de código de barras de ADN sugiere la existencia de tres grupos genéticos que incluyen las seis especies reconocidas analizadas en este estudio, ninguna de las cuales fue recuperada como monofilética. Por otro lado, la morfometría geométrica surge como una herramienta de utilidad para discriminar especies debido a que la variación en caracteres morfométricos tradicionales muestra altos niveles de solapamiento que previene la propuesta de caracteres diagnósticos.
We provide a review of the systematics of Herichthys by evaluating the usefulness of several mitochondrial and nuclear genetic markers together with morphological data. The nDNA next‐generation sequencing ddRAD analysis together with the mtDNA cytochrome b gene provided well‐resolved and well‐supported phylogenies of Herichthys. On the other hand, the nDNA S7 introns have limited resolution and support and the COI barcoding analysis completely failed to recover all but one species of Herichthys as monophyletic. The COI barcoding as currently implemented is thus insufficient to distinguish clearly distinct species in the genus Herichthys that are supported by other molecular markers and by morphological characters. Based on our results, Herichthys is composed of 11 species and includes two main clades (the H. labridens and H. cyanoguttatus species groups). Herichthys bartoni is in many respects the most plesiomorphic species in the genus and has a conflicting phylogenetic position between mtDNA and nDNA markers, where the robust nDNA ddRAD data place it as a rather distant basal member of the H. labridens species group. The mtDNA of H. bartoni is on the other hand only slightly divergent from the sympatric and syntopic H. labridens, and the species thus probably have hybridized in the relatively recent past. The sympatric and syntopic Herichthys steindachneri and H. pame are supported as sister species. The Herichthys cyanoguttatus species group shows two well‐separated basal species (the northernmost H. minckleyi and the southernmost H. deppii) followed by the closely related and centrally distributed species H. cyanoguttatus, H. tepehua, H. carpintis, and H. tamasopoensis whose relationships differ between analyses and show likely hybridizations between themselves and the two basal species as suggested by conflicts between DNA analyses. Several instances of introgressions/hybridizations have also been found between the two main clades of Herichthys.
Phylogeographic studies of different montane biological groups in Mexico have revealed complex patterns in a broad scale but an absence of genetic structure within local mountain systems such as the Sierra Madre Occidental. In this study, we estimate the genetic structure and demographic history of the endemic land snail Humboldtiana durangoensis within this mountain range. Nine polymorphic microsatellite loci in 178 individuals from 16 localities throughout the complete geographic distribution were analyzed. Strong deviations from Hardy–Weinberg equilibrium and low levels of heterozygosity were detected in the seven genetic clusters. The gene flow between two of the main geographic regions (North and South) was symmetric (≈4 individuals). In addition, the analysis detected changes in the effective population size indicating that both geographic regions experienced a drastic reduction in their effective population size probably associated with the Pleistocene climatic changes.
Fishes of the genus Herichthys are the only representatives of the family Cichlidae to have colonized the Neartic region. In this study, we used DNA barcode of 64 individuals of the Herichthys bartoni species group to test the monophyly of the species and the efficiency of this tool to discriminate among species. The Bayesian phylogenetic tree and the neighbour joining (NJ) tree obtained from the Kimura two-parameter model (K2P) give similar topologies. DNA barcoding resolution was very poor (25%). Additionally, the low levels of genetic divergence among taxa preclude the use of threshold values as has been suggested in earlier studies.
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