Microbiomes as modulators of Drosophila melanogaster homeostasis and disease

Lesperance, Danielle N. A.; Broderick, Nichole A.

doi:10.1016/j.cois.2020.03.003

Cited by 57 publications

(50 citation statements)

References 43 publications

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“…Interestingly, some but not all Lactobacillus spp. produce diaminopimelic acid (DAP)-containing peptidoglycan 60 which is known to activate Imd pathway signaling (leading to downstream production of AMPs) in insects via select PGRP binding 61 . Corroborating this, past work has shown that Lp39 (one of the LX3 strains used in this study) produces DAP-type peptidoglycan that can potently upregulate host AMP gene expression via Imd pathway signaling in D. melanogaster 19 .…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees

et al. 2020

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Widespread antibiotic usage in apiculture contributes substantially to the global dissemination of antimicrobial resistance and has the potential to negatively influence bacterial symbionts of honey bees (Apis mellifera). Here, we show that routine antibiotic administration with oxytetracycline selectively increased tetB (efflux pump resistance gene) abundance in the gut microbiota of adult workers while concurrently depleting several key symbionts known to regulate immune function and nutrient metabolism such as Frischella perrera and Lactobacillus Firm-5 strains. These microbial changes were functionally characterized by decreased capped brood counts (marker of hive nutritional status and productivity) and reduced antimicrobial capacity of adult hemolymph (indicator of immune competence). Importantly, combination therapy with three immunostimulatory Lactobacillus strains could mitigate antibiotic-associated microbiota dysbiosis and immune deficits in adult workers, as well as maximize the intended benefit of oxytetracycline by suppressing larval pathogen loads to near-undetectable levels. We conclude that microbial-based therapeutics may offer a simple but effective solution to reduce honey bee disease burden, environmental xenobiotic exposure, and spread of antimicrobial resistance.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees

et al. 2020

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“…The underlying mechanisms remain unknown, but at higher larval densities, chemical changes (e.g., pH) likely favour the growth of some microbial strains over others (Gibson et al 1988;Rousk et al 2009;Russell and Dombrowski 1980). Moreover, microbial strains might interact with each other via metabolites in order to promote growth of a cohort of strains which together, influence larva's development and life history trait (Consuegra et al 2020;Lesperance and Broderick 2020;Sommer and Newell 2019). Given that a large proportion of microbes are acquired from interactions between the host and the surrounding environment, larval density might be important, yet an overlooked factor, in modulating host-microbe interactions (Broderick et al 2004;Colman et al 2012;Tang et al 2012;Vacchini et al 2017;Yun et al 2014;Zhang et al 2018;Zhao et al 2017).…”

Section: Interactions Between Host and Microbesmentioning

confidence: 99%

“…Below, we summarise the main consequences of host-microbe interactions (especially microbes in the gut) to insect life-history trait expression while discussing potential ways through which larval population density could influence these effects. Note that our goal was not to review the (gut) microbe literature as extensive reviews have been published elsewhere [e.g., (Bahrndorff et al 2016;Bordenstein and Theis 2015;Douglas 2009Douglas , 2015Douglas , 2019Lesperance and Broderick 2020;Lewis and Lizé 2015)]. Instead, our aim is to demonstrate potential links between population density and (gut) microbe effects on fitness.…”

Section: Interactions Between Host and Microbesmentioning

confidence: 99%

Integrative developmental ecology: a review of density-dependent effects on life-history traits and host-microbe interactions in non-social holometabolous insects

Ponton

Morimoto

2020

Evol Ecol

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Population density modulates a wide range of eco-evolutionary processes including inter- and intra-specific competition, fitness and population dynamics. In holometabolous insects, the larval stage is particularly susceptible to density-dependent effects because the larva is the resource-acquiring stage. Larval density-dependent effects can modulate the expression of life-history traits not only in the larval and adult stages but also downstream for population dynamics and evolution. Better understanding the scope and generality of density-dependent effects on life-history traits of current and future generations can provide useful knowledge for both theory and experiments in developmental ecology. Here, we review the literature on larval density-dependent effects on fitness of non-social holometabolous insects. First, we provide a functional definition of density to navigate the terminology in the literature. We then classify the biological levels upon which larval density-dependent effects can be observed followed by a review of the literature produced over the past decades across major non-social holometabolous groups. Next, we argue that host-microbe interactions are yet an overlooked biological level susceptible to density-dependent effects and propose a conceptual model to explain how density-dependent effects on host-microbe interactions can modulate density-dependent fitness curves. In summary, this review provides an integrative framework of density-dependent effects across biological levels which can be used to guide future research in the field of ecology and evolution.

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“…Gut microbes have important functions in host development, immunity, intestinal homeostasis, and metabolism across model organisms (1)(2)(3)(4). There is growing appreciation for the gut microbiome's role in host nutritional intake, with effects through both microbial catabolism of nutrients and biosynthesis of metabolites (5)(6)(7).…”

Section: Downloaded Frommentioning

confidence: 99%

Gut Bacteria Mediate Nutrient Availability in Drosophila Diets

Lesperance

Broderick

2020

Appl Environ Microbiol

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Drosophila melanogaster gut microbes play important roles in host nutritional physiology. However, these associations are often indirect and studies typically are in the context of specialized nutritional conditions, making it difficult to discern how microbiome-mediated impacts translate to physiologically relevant conditions, in the laboratory or nature. In this study, we quantified changes in dietary nutrients due to D. melanogaster gut bacteria on three artificial diets and a natural diet of grapes. We show that in all four diet conditions, bacteria altered protein, carbohydrates, and moisture of the food substrate. In depth analysis on one diet revealed that bacteria also increased tryptophan levels, an essential amino acid encountered scarcely in nature. These nutrient changes result in an increased protein to carbohydrate (P:C) ratio in all diets, which we hypothesized to be a significant determinant of microbiome-mediated host nutritional physiology. To test this, we compared life history traits of axenic flies reared on the three artificial diets with increased P:C or continuous bacterial inoculation. We found that, while on some diets an environment of nutritional plenitude had impacts on life history, it did not fully explain all microbiome-associated phenotypes. This suggests that other factors, such as micronutrients and feeding behavior, likely also contribute to life history traits in a diet-dependent manner. Thus, while some bacterial impacts on nutrition occur across diets, others are dictated by unique dietary environments, highlighting the importance of diet-microbiome interactions in D. melanogaster nutritional physiology. Importance Both in the laboratory and in nature, D. melanogaster-associated microbes serve as nutritional effectors, either through production of metabolites or as direct sources of protein biomass. The relationship between the microbiome and resulting host nutritional physiology is significantly impacted by diet composition, yet studies involving D. melanogaster are performed using a wide range of artificial diets, making it difficult to discern which aspects of host-microbe interactions may be universal or diet-dependent. In this study, we utilized three standard D. melanogaster diets and a natural grape diet to form a comprehensive understanding of the quantifiable nutritional changes mediated by the host microbial community. We then altered these artificial diets based on the observed microbe-mediated changes to demonstrate their potential to influence host physiology, allowing us to identify nutritional factors whose effects were either universal for the three artificial diets or dependent on host diet composition.

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Microbiomes as modulators of Drosophila melanogaster homeostasis and disease

Cited by 57 publications

References 43 publications

Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees

Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees

Integrative developmental ecology: a review of density-dependent effects on life-history traits and host-microbe interactions in non-social holometabolous insects

Gut Bacteria Mediate Nutrient Availability in Drosophila Diets

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