Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees

Daisley, Brendan A.; Pitek, Andrew P.; Chmiel, John A.; Gibbons, Shaeley; Chernyshova, Anna M.; Al, Kait F.; Faragalla, Kyrillos M.; Burton, Jeremy P.; Thompson, Graham; Reid, Gregor

doi:10.1038/s42003-020-01259-8

Cited by 63 publications

(73 citation statements)

References 79 publications

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“…Taken together, these results suggest that Lactobacillus, Bifidobacterium, and Gammaproteobacteria may be positively associated with exposure to agrochemicals. Notably, our results differ from a study on European honey bees that reported decreases in several Lactobacillius ASVs following exposure to oxytetracycline (Daisley et al, 2020).…”

Section: Core Microbial Taxa and Tetracycline Treatmentcontrasting

confidence: 99%

Tetracycline exposure alters key gut microbiota in Africanized honey bees (Apis mellifera scutellatax spp.)

Soares

Oliveira

Rodrigues

et al. 2021

Preprint

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Honey bees play a critical role in ecosystem health, biodiversity maintenance, and crop yield. Antimicrobials, such as tetracyclines, are used widely used across agriculture, medicine, and in bee keeping, and bees can be directly or indirectly exposed to tetracycline residues in the environment. In European honey bees, tetracycline exposure has been linked with shifts in the gut microbiota that negatively impact bee health. However, the effects of antimicrobials on Africanized honey bee gut microbiota have not been examined. The aim of this study was to investigate the effects of tetracycline exposure on the gut microbial community of Africanized honey bees (Apis mellifera scutellata x spp), which are important pollinators in South, Central, and North America. Bees (n=1,000) were collected from hives in Areia-PB, Northeastern Brazil, placed into plastic chambers and kept under controlled temperature and humidity conditions. The control group (CON) was fed daily with syrup (10g) consisting of a 1:1 solution of demerara sugar and water, plus a solid protein diet (10g) composed of 60% soy extract and 40% sugar syrup. The tetracycline group (TET) was fed identically but with the addition of tetracycline hydrochloride (450 ug/g) to the sugar syrup. Bees were sampled from each group before (day 0), and after tetracycline exposure (days 3, 6 and 9). Abdominal contents dissected out of each bee underwent DNA extraction and 16S rRNA sequencing (V3-V4) on an Illumina MiSeq. Sequences were filtered and processed through QIIME2 and DADA2. Microbial community composition and diversity and differentially abundant taxa were evaluated by treatment and time. Bee gut microbial composition (Jaccard) and diversity (Shannon) differed significantly and increasingly over time and between CON and TET groups. Tetracycline exposure was associated with decreased relative abundances of Bombella and Fructobacillus, along with decreases in key core microbiota such as Snodgrassella, Gilliamella, Rhizobiaceae, and Apibacter. These microbes are critical for nutrient metabolism and pathogen defense, and decreased abundances of these microbes could negatively affect bee health. Considering the global ecological and economic importance of honey bees as pollinators, it is critical to understand the effects of agrochemicals including antimicrobials on honey bees.

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Section: Core Microbial Taxa and Tetracycline Treatmentcontrasting

confidence: 99%

Tetracycline exposure alters key gut microbiota in Africanized honey bees (Apis mellifera scutellatax spp.)

Soares

Oliveira

Rodrigues

et al. 2021

Preprint

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“…and Gilliamella spp., can survive and proliferate thanks to tet determinants. Recently, Daisley et al (40) found that the routine administration of oxytetracycline increases tetB and tetY abundance in the gut microbiota of adult workers associated with a depletion of the major symbiont taxa. The present study therefore confirms that honeybees represent an impressive reservoir of tetracycline resistance genes, even after two decades without antibiotic treatment.…”

Section: Discussionmentioning

confidence: 99%

Honeybees exposure to veterinary drugs: how the gut microbiota is affected

Baffoni

Alberoni

Gaggìa

et al. 2021

Preprint

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Several studies have outlined that a balanced gut microbiota offers metabolic and protective functions supporting honeybee health and performances. The present work contributes to increasing knowledge on the impact on the honeybee gut microbiota of the administration of three different veterinary drugs (oxytetracicline, sulphonamides and tylosin). The trial was designed with a semi-field approach in micro-hives containing about 500 bees, i.e. in experimental conditions as close as possible to real hives considering the restrictions on the use of antibiotics; 6 replicates were considered for each treatment plus the control. The absolute abundance of the major gut microbial taxa in newly eclosed individuals was studied with qPCR and next generation sequencing. Antimicrobial resistance genes for the target antibiotics were also monitored using a qPCR approach. The results showed that none of the veterinary drugs altered the total amount of gut bacteria, but qualitative variations were observed. Tylosin treatment determined a significant decrease of α- and β-diversity indexes and a strong depletion of the rectum population (lactobacilli and bifidobacteria) while favoring the hindgut population (Gilliamella, Snodgrassella and Frischella spp.). Major changes were also observed in honeybees treated with sulphonamides, with a decrease in Bartonella and Frischella core taxa an increase of Bombilactobacillus spp. and Snodgrassella spp. Conversely, minor effects were observed in oxytetracycline treated honeybees. Monitoring of antibiotic resistance genes confirmed that honeybees represent a great reservoir of tetracycline resistance genes. Tetracycline and sulphonamides resistant genes tended to increase in the gut microbiota population upon antibiotic administration.

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“…Antibiotics can influence the host by altering the species of gut microbiota. Daisley et al [ 119 ] documented the deleterious effects of antibiotics on the gut microbiome, immunity, and productivity of honeybees. Several residues of antibiotics and veterinary chemotherapeutics are detected in honey, showing that honeybees are still exposed to them, despite many countries banning their usage in beekeeping [ 120 , 121 ].…”

Section: Honeybee Microbiotamentioning

confidence: 99%

Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review

Nowak

Szczuka

Górczyńska

et al. 2021

Cells

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Numerous honeybee (Apis mellifera) products, such as honey, propolis, and bee venom, are used in traditional medicine to prevent illness and promote healing. Therefore, this insect has a huge impact on humans’ way of life and the environment. While the population of A. mellifera is large, there is concern that widespread commercialization of beekeeping, combined with environmental pollution and the action of bee pathogens, has caused significant problems for the health of honeybee populations. One of the strategies to preserve the welfare of honeybees is to better understand and protect their natural microbiota. This paper provides a unique overview of the latest research on the features and functioning of A. mellifera. Honeybee microbiome analysis focuses on both the function and numerous factors affecting it. In addition, we present the characteristics of lactic acid bacteria (LAB) as an important part of the gut community and their special beneficial activities for honeybee health. The idea of probiotics for honeybees as a promising tool to improve their health is widely discussed. Knowledge of the natural gut microbiota provides an opportunity to create a broad strategy for honeybee vitality, including the development of modern probiotic preparations to use instead of conventional antibiotics, environmentally friendly biocides, and biological control agents.

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Lactobacillus spp. attenuate antibiotic-induced immune and microbiota dysregulation in honey bees

Cited by 63 publications

References 79 publications

Tetracycline exposure alters key gut microbiota in Africanized honey bees (Apis mellifera scutellatax spp.)

Tetracycline exposure alters key gut microbiota in Africanized honey bees (Apis mellifera scutellatax spp.)

Honeybees exposure to veterinary drugs: how the gut microbiota is affected

Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review

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