Physicochemical characteristics and antimicrobial activity of the extracts propolis of the
ResumoA criação de espécies nativas de abelhas, também conhecidas como abelhas sem ferrão, têm despertado interesse geral na área, em função da facilidade de manejo e da qualidade dos produtos. No entanto, pouco se sabe sobre sua produção e sua biologia e por isto o presente trabalho acompanhou o desenvolvimento de colmeias de Melipona scutellaris, avaliando os seus parâmetros produtivos em ambiente de criação. Como o crescimento de uma colmeia tem como parâmetro o armazenamento de alimento, e como no caso da abelha M. scutellaris este armazenamento é feito em potes construídos com cera, foram feitas contagens dos potes nas colmeias classifi cando-os como: potes abertos, potes fechados e potes vazios, separando-os de acordo com o conteúdo (mel ou pólen). Foram medidas as suas dimensões e anotadas para posterior avaliação dos dados. Com os resultados pôde-se averiguar que as colmeias apresentam quantidades de potes diferentes, variando em função do número de indivíduos que colaboram para este fator. Confi rmou-se que, quando o alimento é escasso no pasto apícola, as abelhas usam o alimento armazenado nos potes, em função da diminuição de matéria-prima na entressafra. Unitermos: abelhas nativas, parâmetros, produção Revista Biotemas, 21 (1), março de 2008 60 A. Evangelista-Rodrigues et al. AbstractEvaluation of the productive development of Melipona scutellaris beehives. Stingless beekeeping has drawn the general attention of beekeepers because of the easy management and the quality of products. Nevertheless, little is known about their production and biology. The present study evaluated the development of Melipona scutellaris hives, considering the production parameters in a beekeeping environment. The growth parameter of hives is food storage. In the case of M. scutellaris, storage is made inside beeswax pots. Therefore, beeswax pots in the hives were classifi ed and counted as nectar pots, pollen pots, open pots, closed pots and empty pots. Hive dimensions were also taken and evaluated. The results indicated that hives show different amounts of pots that vary as a function of the number of individuals in the hive. It was confi rmed that the availability of food resources determines the utilization of food storage, i.e., if there is resource limitation and foraging is compromised, bees utilize the food that is stored inside the pots.
Honey bees play a critical role in ecosystem health, biodiversity maintenance, and crop yield. Antimicrobials, such as tetracyclines, are used widely in agriculture, medicine, and in bee keeping, and bees can be directly or indirectly exposed to tetracycline residues in the environment. In European honey bees, tetracycline exposure has been linked with shifts in the gut microbiota that negatively impact bee health. However, the effects of antimicrobials on Africanized honey bee gut microbiota have not been examined. The aim of this study was to investigate the effects of tetracycline exposure on the gut microbial community of Africanized honey bees (Apis mellifera scutellata x spp.), which are important pollinators in South, Central, and North America. Bees (n = 1,000) were collected from hives in Areia-PB, Northeastern Brazil, placed into plastic chambers and kept under controlled temperature and humidity conditions. The control group (CON) was fed daily with syrup (10 g) consisting of a 1:1 solution of demerara sugar and water, plus a solid protein diet (10 g) composed of 60% soy extract and 40% sugar syrup. The tetracycline group (TET) was fed identically but with the addition of tetracycline hydrochloride (450 μg/g) to the sugar syrup. Bees were sampled from each group before (day 0), and after tetracycline exposure (days 3, 6, and 9). Abdominal contents dissected out of each bee underwent DNA extraction and 16S rRNA sequencing (V3-V4) on an Illumina MiSeq. Sequences were filtered and processed through QIIME2 and DADA2. Microbial community composition and diversity and differentially abundant taxa were evaluated by treatment and time. Bee gut microbial composition (Jaccard) and diversity (Shannon) differed significantly and increasingly over time and between CON and TET groups. Tetracycline exposure was associated with decreased relative abundances of Bombella and Fructobacillus, along with decreases in key core microbiota such as Snodgrassella, Gilliamella, Rhizobiaceae, and Apibacter. These microbes are critical for nutrient metabolism and pathogen defense, and it is possible that decreased abundances of these microbes could negatively affect bee health. Considering the global ecological and economic importance of honey bees as pollinators, it is critical to understand the effects of agrochemicals including antimicrobials on honey bees.
Honey bees play a critical role in ecosystem health, biodiversity maintenance, and crop yield. Antimicrobials, such as tetracyclines, are used widely used across agriculture, medicine, and in bee keeping, and bees can be directly or indirectly exposed to tetracycline residues in the environment. In European honey bees, tetracycline exposure has been linked with shifts in the gut microbiota that negatively impact bee health. However, the effects of antimicrobials on Africanized honey bee gut microbiota have not been examined. The aim of this study was to investigate the effects of tetracycline exposure on the gut microbial community of Africanized honey bees (Apis mellifera scutellata x spp), which are important pollinators in South, Central, and North America. Bees (n=1,000) were collected from hives in Areia-PB, Northeastern Brazil, placed into plastic chambers and kept under controlled temperature and humidity conditions. The control group (CON) was fed daily with syrup (10g) consisting of a 1:1 solution of demerara sugar and water, plus a solid protein diet (10g) composed of 60% soy extract and 40% sugar syrup. The tetracycline group (TET) was fed identically but with the addition of tetracycline hydrochloride (450 ug/g) to the sugar syrup. Bees were sampled from each group before (day 0), and after tetracycline exposure (days 3, 6 and 9). Abdominal contents dissected out of each bee underwent DNA extraction and 16S rRNA sequencing (V3-V4) on an Illumina MiSeq. Sequences were filtered and processed through QIIME2 and DADA2. Microbial community composition and diversity and differentially abundant taxa were evaluated by treatment and time. Bee gut microbial composition (Jaccard) and diversity (Shannon) differed significantly and increasingly over time and between CON and TET groups. Tetracycline exposure was associated with decreased relative abundances of Bombella and Fructobacillus, along with decreases in key core microbiota such as Snodgrassella, Gilliamella, Rhizobiaceae, and Apibacter. These microbes are critical for nutrient metabolism and pathogen defense, and decreased abundances of these microbes could negatively affect bee health. Considering the global ecological and economic importance of honey bees as pollinators, it is critical to understand the effects of agrochemicals including antimicrobials on honey bees.
RESUMO. Com o objetivo de inventariar as plantas visitadas por abelhas Melipona scutellaris L. na Microrregião do Brejo Paraibano, delimitaram-se, 4 linhas de transecção de aproximadamente 1km 2 cada, partindo-se do meliponário constituído por 20 colméias da estação experimental Chã de Jardim da Universidade Federal da Paraíba, no município de Areia, estado da Paraíba. As trilhas foram percorridas uma vez por semana durante todo o período de execução do trabalho (abril a julho), nos horários das 7h30min às 10h30min, no qual todas as plantas em florescimento com até 3m de altura foram observadas, por cerca de 5 minutos, anotando-se a visita das abelhas Uruçu. A conclusão foi que essas abelhas apresentam preferência pela vegetação característica da Mata Atlântica e capoeira em detrimento da vegetação de campo, mostrando-se bastante seletivas com relação à escolha de fontes alimentares. Para os meliponicultores, pode-se sugerir como pasto apícola as plantas: Erytroxylon sp.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.