Microbiome Reengineering by Heat Selection for Rapid Biodegradation of Trichloroethylene with Minimal Vinyl Chloride Formation

Chan, Ai-Lin; Tseng, Chien-Yun; Chen, Tzu-Wen; Lo, Yi-Hsuan; Chang, Shu-Chi

doi:10.1007/s11270-021-05028-z

Cited by 4 publications

(1 citation statement)

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“…The specific response of the microbiome on location to temperature must be investigated; however, most commonly reported temperatures are 20-30 • C with deterioration of the process above 35-40 • C [27][28][29][30][31][32]; thus, thermally enhanced anaerobic dechlorination could be costly, but effective [30]. The highest efficiency of the remediation method will be reflected in the shortening of the time required to reach the target remediation limits through improved biological parameters, e.g., cell growth and (bio)reaction rates [33][34][35] and physical-chemical, e.g., pollutant desorption, their volatilization and (bio)availability [36] and releasing of direct electron donors [35] and thus in lower remediation costs. However, costs could be reduced by replacing conventional heating with some modern and sustainable approach, e.g., geothermal heat pumps and solar heating [37] or synergistic coupling dechlorination remediation with underground thermal energy storage [36,38].…”

Section: Chemical Analysesmentioning

confidence: 99%

Thermally Enhanced Biodegradation of TCE in Groundwater

Najmanová

Steinová

Czinnerová

et al. 2022

Water

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In situ remediation is usually restricted by temperature, lack of substrate for reductive dechlorination (anaerobic respiration), the presence of dehalogenating microorganisms, and specific bedrock conditions. In this work, trichloroethene (TCE) degradation was studied by a number of methods, from physical–chemical analyses to molecular biological tools. The abundance changes in dechlorinating bacteria were monitored using real-time PCR. The functional genes vcrA and bvcA as well as the 16S rRNA specific for representatives of genera Dehalococcoides, Dehalobacter, and Desulfitobacterium were monitored. Furthermore, the sulfate-reducing bacteria and denitrifying bacteria were observed by amplifying the functional genes apsA and nirK. The elevated temperature and the substrate (whey) addition significantly affected TCE dechlorination. The chlorine index decreased after nine weeks from 2.5 to 0.1 at 22 °C, to 1.1 at 17 °C and 1.7 at 12 °C and complete dechlorination was achieved at 22 °C with whey addition. The achieved results of this work show the feasibility and effectiveness of biological dechlorination of TCE enhanced with elevated temperature and whey addition.

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