Depth-resolved microbial diversity and functional profiles of trichloroethylene-contaminated soils for Biolog EcoPlate-based biostimulation strategy

Koner, Suprokash; Chen, Jung‐Sheng; Hsu, Bing-Mu; Rathod, Jagat; Huang, Shing-Tsaan; Chien, H. Y.; Hussain, Bashir; Chan, Michael W.Y.

doi:10.1016/j.jhazmat.2021.127266

Cited by 60 publications

(20 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was found that the biodegradation of sulfamethoxazole was also enhanced when an easily degradable energy supply (acetate) was provided which fostered metabolic activity [37]. Related studies have shown that carbon sources such as some utilization traits exhibit a significant positive correlation with the abundance of chlorane and chloroene degradation pathways [38]. This correlation can, therefore, be interpreted as promoting effective biostimulation through external nutrient amendment to increase biometabolic diversity and activity for efficient in situ degradation of contaminants.…”

Section: Identification Of Efficient Ofl-degrading Bacteriamentioning

confidence: 97%

Changes in microbial community structure and co-metabolism during the domestication of ofloxacin-degrading bacteria

Zhang

Sha

et al. 2022

Environ Sci Eur

View full text Add to dashboard Cite

Background Ofloxacin (OFL) is stable and difficult to degrade. It has been detected in water, soil, and plants throughout the world. This study domesticated OFL-contaminated livestock manure soil with simplified carbon sources to identify flora capable of effectively degrading OFL. The changes in the structural composition and diversity of the microbial community and the functional abundance of the soil flora were analyzed by metagenome sequencing technology. The Biolog-ECO microplate method was used to study the utilization of 31 different carbon sources by selected bacteria and to identify the best co-metabolized carbon source for degradation. Results Amino acid carbon sources were more likely to cause significant changes in community structures with increasing OFL concentrations during the acclimation stage. The abundance of Sphingobacterium decreased from 69.23% to 9.84%, while Alcaligenes increased from 0.27% to 62.79%, and Stenotrophomonas increased from 11.63% to 33.33%, becoming the dominant genus. The results suggested that Stenotrophomonas and Alcaligenes were potential candidate bacteria for the degradation of quinolone antibiotics, such as OFL. Compared with the first stage of acclimation, there was an 87% increase (the concentration was 30 mg·L−1) in the OFL degradation rate by functional flora obtained by gradient acclimation, and the functional abundance of the microbial community also increased and stabilized with the depth of the domestication process. The most significant changes in membrane transport were observed in the functional abundance of the microbial community, and it was found that itaconic acid, Tween 80, and L-aspartic acid could increase the biomass of the microbial community under OFL stress. Conclusion Significant changes in the bacterial composition and functional abundance of the microbial community resulted from the addition of amino acid carbon sources, together with the OFL concentration. Functional flora resulting from domestication were better able to degrade OFL. The addition of a co-metabolic carbon source significantly enhanced the biomass of the functional flora. In this study, co-metabolism was performed by adding specific carbon sources, thus achieving metabolic diversity of functional flora and ultimately efficient biodegradation of OFL. This was an important discovery in the field of microbial remediation of environmental contamination.

show abstract

Section: Identification Of Efficient Ofl-degrading Bacteriamentioning

confidence: 97%

Changes in microbial community structure and co-metabolism during the domestication of ofloxacin-degrading bacteria

Zhang

Sha

et al. 2022

Environ Sci Eur

View full text Add to dashboard Cite

show abstract

“…Higher diversity often increases the consumption of different substrates compared to deep soil, where microbial diversity is restricted [66,67]. Additionally, it is indicated that the soil depth gradient reduced both nutrient availability and the oxygen rate, which had a negative effect on soil bacteria and the regulation of their metabolic process [68,69]. The average well color development (AWCD) is a vital index of soil microbiota usage of carbon sources and reflects the physiological functions of soil microbial diversity [69,70].…”

Section: Biochar Effect On Soil Carbon Sources and Indicesmentioning

confidence: 99%

The Impact of Swine Manure Biochar on the Physical Properties and Microbial Activity of Loamy Soils

Ayaz

Feizienė

Feiza

et al. 2022

Plants

View full text Add to dashboard Cite

Biochar has been proven to influence soil hydro-physical properties, as well as the abundance and diversity of microbial communities. However, the relationship between the hydro-physical properties of soils and the diversity of microbial communities is not well studied in the context of biochar application. The soil analyzed in this study was collected from an ongoing field experiment (2019–2024) with six treatments and three replications each of biochar (B1 = 25 t·ha−1 and B0 = no biochar) and nitrogen fertilizer (N1 = 160, N2 = 120 kg·ha−1, and N0 = no fertilizer). The results show that biochar treatments (B1N0, B1N1, and B1N2) significantly improved the soil bulk density and total soil porosity at different depths. The B1N1 treatment substantially enhanced the volumetric water content (VMC) by 5–7% at −4 to −100 hPa suction at 5–10 cm depth. All three biochar treatments strengthened macropores by 33%, 37%, and 41%, respectively, at 5–10 cm depth and by 40%, 45%, and 54%, respectively, at 15–20 cm depth. However, biochar application significantly lowered hydraulic conductivity (HC) and enhanced carbon source utilization and soil indices at different hours. Additionally, a positive correlation was recorded among carbon sources, indices, and soil hydro-physical properties under biochar applications. We can summarize that biochar has the potential to improve soil hydro-physical properties and soil carbon source utilization; these changes tend to elevate fertility and the sustainability of Cambisol.

show abstract

“…The average well colour development (AWCD) curve was plotted after normalizing the absorbance of each well against the reading of the water-containing well. The negative values were considered zero (Koner et al, 2022).…”

Section: Phenotypic Analysismentioning

confidence: 99%

Microbial electrochemical systems for enhanced degradation of glyphosate: Electrochemical performance, degradation efficiency, and analysis of the anodic microbial community

Rafieenia

Mahmoud

El‐Gohary

et al. 2022

Preprint

View full text Add to dashboard Cite

Glyphosate, one of the most used herbicides worldwide, is known as an aquatic contaminant of concern and can present adverse impacts in the agroecosystems. In this study, we investigated the degradation of glyphosate in microbial electrochemical systems (MESs), analysed the microbial composition of enriched anodic biofilms, and compared them with microbial communities of non-MESs enriched cultures. MESs supported higher glyphosate degradation (68.41 % to 73.90 %) compared to non-MESs cultures (48.88 %). The Linear Sweep Voltammetry (LSV) analysis showed that MESs operated at +300 mV, produced a maximum current of 0.611 mA, which was the highest among all the applied voltages. 16S amplicon sequencing revealed a significant difference in microbial community composition of MESs anodic biofilms and non-MESs enriched communities. The anodic biofilms were dominated by Rhodococcus (51.26 %), Pseudomonas (10.77 %), and Geobacter (8.67 %) while in non-MESs cultures, methanogens including Methanobrevibacter (51.18 %), and Methanobacterium (10.32 %), were the dominant genera. The present study suggested that MESs could be considered as a promising system for glyphosate degradation.

show abstract

Depth-resolved microbial diversity and functional profiles of trichloroethylene-contaminated soils for Biolog EcoPlate-based biostimulation strategy

Cited by 60 publications

References 78 publications

Changes in microbial community structure and co-metabolism during the domestication of ofloxacin-degrading bacteria

Changes in microbial community structure and co-metabolism during the domestication of ofloxacin-degrading bacteria

The Impact of Swine Manure Biochar on the Physical Properties and Microbial Activity of Loamy Soils

Microbial electrochemical systems for enhanced degradation of glyphosate: Electrochemical performance, degradation efficiency, and analysis of the anodic microbial community

Contact Info

Product

Resources

About