Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels

Guimarães, Rafaela Araújo; Pherez-Perrony, Paul Esteban; Müller, Henry; Berg, Gabriele; Medeiros, Flávio Henrique Vasconcelos de; Cernava, Tomislav

doi:10.1016/j.biocontrol.2020.104370

Cited by 10 publications

(23 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The obtained results validated both the low number of bacteria in the samples and the percentage of Firmicutes compared to the total of eubacteria for most analyzed samples. (ii) The overall structure of the microbiota of the embryos, with a great abundance of Proteobacteria, followed by Actinobacteria, Bacteroidetes, and Firmicutes is in accordance with the results obtained by Guimaraes and colleagues [58] when analyzing the microbiota of maize ears. (iii)The number of reads and OTUs obtained from the sequencing is comparable to those obtained by a previous work carried out on embryos of wheat [57].…”

Section: Discussionsupporting

confidence: 89%

Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot

et al. 2021

View full text Add to dashboard Cite

Locally adapted maize accessions (landraces) represent an untapped resource of nutritional and resistance traits for breeding, including the shaping of distinct microbiota. Our study focused on five different maize landraces and a reference commercial hybrid, showing different susceptibility to fusarium ear rot, and whether this trait could be related to particular compositions of the bacterial microbiota in the embryo, using different approaches. Our cultivation-independent approach utilized the metabarcoding of a portion of the 16S rRNA gene to study bacterial populations in these samples. Multivariate statistical analyses indicated that the microbiota of the embryos of the accessions grouped in two different clusters: one comprising three landraces and the hybrid, one including the remaining two landraces, which showed a lower susceptibility to fusarium ear rot in field. The main discriminant between these clusters was the frequency of Firmicutes, higher in the second cluster, and this abundance was confirmed by quantification through digital PCR. The cultivation-dependent approach allowed the isolation of 70 bacterial strains, mostly Firmicutes. In vivo assays allowed the identification of five candidate biocontrol strains against fusarium ear rot. Our data revealed novel insights into the role of the maize embryo microbiota and set the stage for further studies aimed at integrating this knowledge into plant breeding programs.

show abstract

Section: Discussionsupporting

confidence: 89%

Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot

et al. 2021

View full text Add to dashboard Cite

show abstract

“…In addition, by changing the environmental conditions, the structure and function of microbiomes can be shifted ( Berg and Smalla, 2009 ). In general, various treatments were shown to induce similar patterns and microbiome shifts ( Cernava et al, 2019 ; Guimarães et al, 2020 ). Surprisingly, breeding was shown to cause similar, long-term microbiome shifts as well ( Pérez-Jaramillo et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Microbiome Modulation—Toward a Better Understanding of Plant Microbiome Response to Microbial Inoculants

et al. 2021

Self Cite

View full text Add to dashboard Cite

Plant-associated microorganisms are involved in important functions related to growth, performance and health of their hosts. Understanding their modes of action is important for the design of promising microbial inoculants for sustainable agriculture. Plant-associated microorganisms are able to interact with their hosts and often exert specific functions toward potential pathogens; the underlying in vitro interactions are well studied. In contrast, in situ effects of inoculants, and especially their impact on the plant indigenous microbiome was mostly neglected so far. Recently, microbiome research has revolutionized our understanding of plants as coevolved holobionts but also of indigenous microbiome-inoculant interactions. Here we disentangle the effects of microbial inoculants on the indigenous plant microbiome and point out the following types of plant microbiome modulations: (i) transient microbiome shifts, (ii) stabilization or increase of microbial diversity, (iii) stabilization or increase of plant microbiome evenness, (iv) restoration of a dysbiosis/compensation or reduction of a pathogen-induced shift, (v) targeted shifts toward plant beneficial members of the indigenous microbiota, and (vi) suppression of potential pathogens. Therefore, we suggest microbiome modulations as novel and efficient mode of action for microbial inoculants that can also be mediated via the plant.

show abstract

“…They exhibit the ability to stimulate plant growth by phosphate solubilization and pathogen antagonization [13]. An application of fungicides with the Bacillus subtilis strain was identified as a promising strategy for controlling Fusarium verticillioides-caused diseases [48]. Moreover, it is commonly accepted that seed endophytes could spread systemically through the seeding phyllosphere plant, exit the root, and colonize the rhizosphere [13,21], playing vital roles in plant health, disease resistance, and growth-promotion [13,49].…”

Section: Predictive Functional Profiling In the Seed Microbiomementioning

confidence: 99%

Anemochore Seeds Harbor Distinct Fungal and Bacterial Abundance, Composition, and Functional Profiles

Liu

Cai

et al. 2022

JoF

View full text Add to dashboard Cite

Many plants adapted to harsh environments have evolved low seed mass (‘light seeds’) with specific dispersal strategies, primarily either by wind (anemochory) or water (hydrochory). However, the role of their seed microbiota in their survival, and their seed microbial abundance and structure, remain insufficiently studied. Herein, we studied the light seed microbiome of eight anemochores and two hydrochores (as controls) collected from four provinces in China, using qPCR and metagenomic sequencing targeting both bacteria and fungi. Substantial variations were found for seed endophytic fungi (9.9 × 1010~7.3 × 102 gene copy numbers per seed) and bacteria (1.7 × 1010~8.0 × 106). Seed microbial diversity and structure were mainly driven by the plant genotype (species), with weak influences from their host plant classification level or dispersal mode. Seed microbial composition differences were clear at the microbial phylum level, with dominant proportions (~75%) for Proteobacteria and Ascomycota. The light seeds studied harbored unique microbial signatures, sharing only two Halomonas amplicon sequence variants (ASVs) and two fungal ASVs affiliated to Alternaria and Cladosporium. A genome-level functional profile analysis revealed that seed bacterial microbiota were enriched in amino acid, nucleoside, and nucleotide biosynthesis, while in fungal communities the generation of precursor metabolites and respiration were more highly represented. Together, these novel insights provide a deeper understanding of highly diversified plant-specific light seed microbiota and ecological strategies for plants in harsh environments.

show abstract

Microbiome-guided evaluation of Bacillus subtilis BIOUFLA2 application to reduce mycotoxins in maize kernels

Cited by 10 publications

References 57 publications

Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot

Bacterial Communities in the Embryo of Maize Landraces: Relation with Susceptibility to Fusarium Ear Rot

Microbiome Modulation—Toward a Better Understanding of Plant Microbiome Response to Microbial Inoculants

Anemochore Seeds Harbor Distinct Fungal and Bacterial Abundance, Composition, and Functional Profiles

Contact Info

Product

Resources

About