Microbiome differences in periodontal, peri-implant, and healthy sites: a cross-sectional pilot study

Barbagallo, Giovanni; Santagati, Maria; Guni, Alaa; Torrisi, Paolo; Spitale, Ambra; Stefani, Stefania; Ferlito, S; Nibali, Luigi

doi:10.1007/s00784-021-04253-4

Cited by 25 publications

(24 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These genera could serve as indicators of disease progression. Some of these genera have been regarded as pathogenic peri‐implant microbes, such as Filifactor (Barbagallo et al, 2021); Treponema , Fretibacterium , and Desulfobulbus (Korsch et al, 2021; Polymeri et al, 2021); Tannerella (Wang et al, 2020); Porphyromonas (Yu et al, 2019). Additionally, we observed several genera that were not reported in peri–implant disease previously.…”

Section: Discussionmentioning

confidence: 99%

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Sun

Wei

et al. 2023

Clinical Oral Implants Res

View full text Add to dashboard Cite

ObjectivesThis study aimed to investigate the relationship between microbial communities and the severity of peri‐implant mucosal bleeding in peri‐implant mucositis.Materials and MethodsSubmucosal plaque samples were collected from 54 implants divided into the healthy implant (HI) group, peri‐implant mucositis (PM) group, and peri‐implantitis (PI) group. Sequencing of 16S rRNA was performed using the Illumina MiSeq platform. Alpha diversity (i.e., Shannon and Chao index) and beta diversity were used to measure microbial diversity within and between microbial communities, respectively. Differences in microbial taxa between groups were assessed via linear discriminate analysis effect size. Correlation between the modified sulcus bleeding index (mSBI) and microbial dysbiosis index (MDI) was examined using Spearman correlation analysis and linear models.ResultsThe submucosal bacterial richness (Chao index) was positively correlated with the mean mSBI in the PM group. As the mean mSBI increased in the PM group, the beta diversity became closer to that of the PI group. In the PM group, the abundances of 47 genera were significantly correlated with the mean mSBI, and the MDI was positively associated with the mean mSBI. Fourteen of the forty‐seven genera were discriminative taxa between the HI and PI groups, and the abundances of these biomarkers became closer to those in the PI group in the progression of peri‐implant disease.ConclusionsA higher mSBI value corresponded to a higher risk of microbial dysbiosis in peri‐implant mucositis. The biomarkers identified may be useful for monitoring the progression of peri‐implant disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Sun

Wei

et al. 2023

Clinical Oral Implants Res

View full text Add to dashboard Cite

show abstract

“…Recently, Barbagallo et al [ 39 ] pointed out that Peptostreptococcaceae XIG-1 and Peptostreptococcaceae XIG-6 were over-abundant in peri-implantitis compared to healthy teeth. Meanwhile, Wang et al [ 40 ] found that the genus of Peptostreptococcaceae XIG-9 was significantly more abundant at peri-implant mucositis sites with suppuration. In our study, Peptostreptococcaceae XIG-1 was detected with significantly higher relative abundance at peri-implantitis sites, which indicates that species of Peptostreptococcus reside in the healthy implant site as commensals, but may be opportunistic pathogens with potential correlations with peri-implantitis.…”

Section: Discussionmentioning

confidence: 99%

The Characteristics of Microbiome and Cytokines in Healthy Implants and Peri-Implantitis of the Same Individuals

Song

Jiang

et al. 2022

JCM

View full text Add to dashboard Cite

Aim: To characterize the profile of submucosal microbiome and cytokine levels in peri-implant crevicular fluid (PICF) from clinically healthy implants and peri-implantitis in the same individuals. Material and Methods: A total of 170 patients were screened and, finally, 14 patients with at least one healthy implant and one peri-implantitis implant were included. Submucosal microbiota and cytokines from 28 implants were analyzed using 16S rRNA gene sequencing and multifactor assays, respectively. Correlations of clinical indexes and microbiota or cytokines were analyzed using Spearman’s correlation coefficient. A random forest classification model was constructed. Results: Peri-implantitis sites harbored higher microbial diversity, as well as more Gram-negative bacteria and anaerobic bacteria, compared with healthy implants sites. The genera of Peptostreptococcaceae XIG-1, Treponema, Porphyromonas, and Lachnospiraceae G-8, as well as the cytokines of IL-17A, IL-6, IL-15, G-CSF, RANTES, and IL-1β were significantly higher in peri-implantitis than healthy implants. Furthermore, these genera and cytokines had positive relationships with clinical parameters, including probing depth (PD), bleeding on probing (BOP), and marginal bone loss (MBL). The classification model picked out the top 15 biomarkers, such as IL-17A, IL-6, IL-15, VEGF, IL-1β, Peptostreptococcaceae XIG-1, Haemophilus, and Treponema, and obtained an area under the curve (AUC) of 0.85. Conclusions: There are more pathogenic bacteria and inflammatory cytokines in peri-implantitis sites, and biomarkers could facilitate the diagnosis of peri-implantitis.

show abstract

“…High abundance of Streptococcus was positively associated with the risk of esophageal squamous cell carcinoma [ 57 ]. Rothia , a normal microbiome of the human mouth, was generally associated with good oral health [ 58 , 59 ], and has been reported to be less abundant in patients with oral cancer [ 60 ]. A study assessing the relationship between the viral copy numbers of EBV and the abundances of salivary microbes found that the abundances of Lachnoanaerobaculum and Stomatobaculum were negatively correlated with the viral copy numbers of EBV [ 61 ].…”

Section: Discussionmentioning

confidence: 99%

The Oral Microbiome as Mediator between Oral Hygiene and Its Impact on Nasopharyngeal Carcinoma

Liu

Liao

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Oral hygiene and the alteration of the oral microbiome have been linked to nasopharyngeal carcinoma (NPC). This study aimed to investigate whether the oral microbiome plays a mediating role in the relationship between oral hygiene and NPC, and identify differential microbial taxonomies that potentially mediated this association. We conducted a case–control study that involved 218 NPC patients and 192 healthy controls. The 16S rRNA gene sequencing of the V4 region was performed to evaluate the composition of the oral microbiome. Mediation analysis was applied to explore the relationship among oral hygiene, the oral microbiome and NPC. We found that dental fillings and poor oral hygiene score were associated with increased risks of NPC (OR = 2.51 (1.52–4.25) and OR = 1.54 (1.02–2.33)). Mediation analysis indicated that dental fillings increased the risk of NPC by altering the abundance of Erysipelotrichales, Erysipelotrichaceae, Solobacterium and Leptotrichia wadei. In addition, Leptotrichia wadei also mediated the association between oral hygiene score and the risk of NPC. Our study confirmed that poor oral hygiene increased the risk of NPC, which was partly mediated by the oral microbiome. These findings might help us to understand the potential mechanism of oral hygiene influencing the risk of NPC via the microbiome.

show abstract

Microbiome differences in periodontal, peri-implant, and healthy sites: a cross-sectional pilot study

Cited by 25 publications

References 46 publications

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

Mucosal bleeding correlates with submucosal microbial dysbiosis in peri‐implant mucositis of patients with periodontitis

The Characteristics of Microbiome and Cytokines in Healthy Implants and Peri-Implantitis of the Same Individuals

The Oral Microbiome as Mediator between Oral Hygiene and Its Impact on Nasopharyngeal Carcinoma

Contact Info

Product

Resources

About