Microbiological study of effects of solid organic waste (chicken droppings and sheep manure) decomposed in the soil used for<i>Pisum sativum</i>cultivation

Barnossi, Azeddin El; Saghrouchni, Hamza; Moussaid, Fatimazahrae; Chahmi, Naima; Housseini, Abdelilah Iraqi

doi:10.1080/00207233.2019.1704116

Cited by 9 publications

(4 citation statements)

References 44 publications

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“…For performing multiple comparisons, Student's t-test was used as a post hoc test. Statistically, a significant difference was considered at a threshold of α = 5% [36]. The variables that were considered as a factor in each assay are presented in Table 4.…”

Section: Discussionmentioning

confidence: 99%

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

et al. 2021

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Background: Gramineae damping-off disease is a growing problem worldwide, which affects a large range of seedlings in nurseries, glasshouses, gardens, crops, forests and untimely generates a heavy economic impact on the agriculture and related sectors. Objectives: The present study was conducted to evaluate the preventive potential of carvacrol on germination of Fusarium oxysporum, Neocosmospora solani, and Microdochium nivale spores as responsible agents for Lolium perenne seeds damping-off disease. Material and methods: Macrodilution method in agar medium, spore germination, spore destruction, and preventive treatment bioassays were used to achieve this goal. Results: The minimum inhibitory concentration (MIC) of carvacrol vs. tested strains existed in the range of 0.25–0.5 mg/mL. Carvacrol used in concentrations ranging from 0.2 to 0.4 mg/mL inhibited the germination of all fungal spores in a dose-dependent manner. Carvacrol showed a very strong sporicidal effect against all studied fungal strains, and this effect was well confirmed by microscopic observations. The percentage of growth inhibition was found to be strictly correlated to carvacrol dose up vs. all strains. Carvacrol increased the emergence of L. perenne seeds when compared to both uninfested and infested seeds. Conclusion: Based on the results obtained, carvacrol fulfills the requirement for being a natural alternative agent to fight Gramineae seedlings’ damping-off caused by fungal species without adverse effects on the plants.

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Section: Discussionmentioning

confidence: 99%

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

et al. 2021

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“…One gram from each sample was transferred to test tube containing 9 mL of 0.9% NaCl. From this solution, a series of dilutions (10 -2 to 10 -6 ) were made ( El Barnossi et al., 2019 ). From each dilution 0.1 mL was spread on Petri plates containing MEA medium.…”

Section: Methodsmentioning

confidence: 99%

Potential of carvacrol as plant growth-promotor and green fungicide against fusarium wilt disease of perennial ryegrass

Saghrouchni

Barnossi²,

Mssillou³

et al. 2023

Front. Plant Sci.

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Perennial ryegrass (Lolium perenne L.) is a valuable forage and soil stabilisation crop. Perennial crops have long been associated with good environmental performance and ecosystem stability. Vascular wilt diseases caused by Fusarium species are the most damaging plant diseases affecting both woody perennials and annual crops. Therefore, the aim of the present study was the assessment of the preventive and growth-promoting effects of carvacrol against Fusarium oxysporum, F. solani, and F. nivale (phylogenetically analyzed on the basis of internal transcribed spacer (ITS) regions) causing vascular wilt of ryegrass in vitro and under greenhouse conditions. To accomplish this aim, various parameters were monitored including coleoptile development, rhizogenesis, the incidence of coleoptile lesions, disease index, the visual appearance of ryegrass health, ryegrass organic matter and soil fungal load. The results obtained showed that F. nivale was highly harmful to ryegrass seedlings compared to other Fusarium species. Furthermore, carvacrol with 0.1 and 0.2 mg/mL protected significantly the seedlings against Fusarium wilt diseases both in vitro and in the greenhouse. Simultaneously, carvacrol also functioned as a seedling growth promoter, as is reflected in all monitored parameters, such as the recovery of seedling height and root length, and the development of new leaf buds and secondary roots. Carvacrol proved to be effective plant growth promoter and a bio-fungicide against Fusarium vascular diseases.

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“…Potato dextrose agar (PDA) and malt extract broth (ME) were obtained from Biokar, France. All media were autoclaved at 121 °C for 20 min and stored at 4 °C until use [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Treatment with Glyphosate Induces Tolerance of Citrus Pathogens to Glyphosate and Fungicides but Not to 1,8-Cineole

et al. 2022

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During the postharvest period, citrus fruits are exposed to Penicillium italicum, Penicillium digitatum, and Geotrichum candidum. Pesticides such as imazalil (IMZ), thiabendazole (TBZ), orthophenylphenol (OPP), and guazatine (GUA) are commonly used as antifungals. Glyphosate (GP) is also used in citrus fields to eliminate weed growth. The sensitivity of fungal pathogens of citrus fruit to these pesticides and 1,8-cineole was evaluated, and the effect of GP on the development of cross-resistance to other chemicals was monitored over a period of 3 weeks. IMZ most effectively inhibited the mycelial growth and spore germination of P. digitatum and P. italicum, with minimum inhibitory concentrations (MICs) of 0.01 and 0.05 mg/mL, respectively, followed by 1,8-cineole, GP, and TBZ. 1,8-Cineole and GP more effectively inhibited the mycelial growth and spore germination of G. candidum, with minimum inhibitory concentrations (MICs) of 0.2 and 1.0 mg/mL, respectively, than OPP or GUA. For the spore germination assay, all substances tested showed a total inhibitory effect. Subculturing the fungal strains in culture media containing increasing concentrations of GP induced fungal tolerance to GP as well as to the fungicides. In soil, experiments confirmed that GP induced the tolerance of P. digitatum to TBZ and GP and the tolerance of P. italicum to IMZ, TBZ, and GP. However, no tolerance was recorded against 1,8-cineole. In conclusion, it can be said that 1,8-cineole may be recommended as an alternative to conventional fungicides. In addition, these results indicate that caution should be taken when using GP in citrus fields.

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Microbiological study of effects of solid organic waste (chicken droppings and sheep manure) decomposed in the soil used forPisum sativumcultivation

Cited by 9 publications

References 44 publications

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

Carvacrol: A Promising Environmentally Friendly Agent to Fight Seeds Damping-Off Diseases Induced by Fungal Species

Potential of carvacrol as plant growth-promotor and green fungicide against fusarium wilt disease of perennial ryegrass

Treatment with Glyphosate Induces Tolerance of Citrus Pathogens to Glyphosate and Fungicides but Not to 1,8-Cineole

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