Microbiological investigation of Escherichia coli isolates from cloacal and feacal swabs of broiler chickens for extended spectrum beta lactamase (ESBL) enzymes

Emmanuel, Eze; Emmanuel, Nwakeze; Anthonia, Oji; Chika, Ejikeugwu; Iroha,

doi:10.9790/3008-0759699

Cited by 6 publications

(1 citation statement)

References 11 publications

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“…The study (Garcia-Graells et al, 2013) conducted on a subset of E. coli isolates from poultry recorded 82.45 per cent of them were positive for ESBL and also recorded that CTX-M (ESBL) is predominantly produced by the commensal E. coli. Further, Emmanuel et al (2013) has compared the ESBL production by E. coli isolated from cloacal samples (74 per cent) with faecal swabs (67 per cent) from the same poultry. Moreover, different strains of E. coli in the same poultry have shown different resistance types (ESBL and AmpC genes).…”

Section: Resultsmentioning

confidence: 99%

Distribution of TET, AAC and CTX-M Genes among Antibiotic Resistant Escherichia coli Isolated From Poultry under Various Farming System of A and N Islands

Sunder

Shunmugaperumal

Bhowmick

et al. 2021

IJAR

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Background: Transmission of antibiotic resistance from animal food chain to human through animal food-borne pathogens have led to increased public concern. Wider surveillance on prevalence of antibiotic resistance in E. coli will provide information on evolution of resistance in various geographical locations. The purpose of this study was to investigate the presence of antimicrobial resistance of E. coli isolates from poultry under various farming system in A and N Islands and resistance genes of tet, ctx-M and aac encoding the isolates. Methods: Isolates were obtained from cloacal swabs in poultry under various farming systems and tested against major antimicrobial derivatives to study multi drug resistance. The presence of genes associated with resistance to tetracycline (tet A), ESBL (CTX-M) and Gentamycin (aac(3)-IV) were determined by PCR. Result: A total of 126 cloacal samples were analysed out of which 31.38% of the E.coli isolates from poultry under various farming systems were producing extended spectrum beta-lactamases and were multiple antimicrobial resistant. Poultry birds of commercial farms showed higher resistance levels (37.5%) than organised farms (24.76%) and desi birds (31.88%). Results indicate a high level of multi-drug resistance is emerging even in desi birds. It is suggested that an antimicrobial resistance surveillance program is needed in A and N Islands in order to detect bacterial resistance among rural poultry production as the 80 percentage of total poultry population belong to desi birds.

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Section: Resultsmentioning

confidence: 99%

Distribution of TET, AAC and CTX-M Genes among Antibiotic Resistant Escherichia coli Isolated From Poultry under Various Farming System of A and N Islands

Sunder

Shunmugaperumal

Bhowmick

et al. 2021

IJAR

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Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Ugwu¹,

Anyanwu²,

Ugwu³

et al. 2015

Not Sci Biol

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This study was conducted to isolate generic extended-spectrum β-lactam (ESBL)-resistant enterobacteria from pigs reared in Enugu State Southeast, Nigeria and determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 190, randomly selected, apparently healthy pigs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 2 µg/ml of cefotaxime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 46 ESBL-resistant enterobacterial isolates, 4 (8.7%) were Escherichia coli, 11 (23.9%) were Salmonella species, while 31 (67.4%) were Klebsiella species. Resistance of the Salmonella isolates was 45.5% to ciprofloxacin, 36.4% to ofloxacin and levofloxacin, 9.1% to norfloxacin, amikacin and gentamicin, 27.3% to streptomycin, 72.7% to chloramphenicol and 90.9% to tetracycline. Resistance of the Klebsiella isolates was 93.5% to ampicillin, 12.9% to ciprofloxacin, 19.4% to ofloxacin and levofloxacin, 9.7% to norfloxacin and streptomycin, 64.5% to chloramphenicol and 38.7% to tetracycline. Resistance of the E. coli isolates was 100% to gentamicin, 75% to ampicillin and streptomycin, 50% to ciprofloxacin, norfloxacin, chloramphenicol and tetracycline, and 25% to ofloxacin, levofloxacin and amikacin. All the isolates were resistant to ceftriaxone, cefotaxime, ceftazidime, cefepime, cefpodoxime, amoxicillin/clavulanic acid and aztreonam. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 54.8% for Klebsiella, 90.9% for Salmonella and 100% for E. coli, respectively. This study has shown that pigs reared in Enugu State Southeast, Nigeria, are colonized by ESBL-resistant Enterobactericeae and are potential reservoirs and disseminators of these organisms.

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Prevalence and Antibiogram of Generic Enterococci in Ready-to-Slaughter Beef Cattle

Anyanwu

Obetta

2015

Not Sci Biol

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Rectal swabs were collected from 95, systematic randomly selected, apparently healthy beef cattle, in order to isolate generic enterococci in Nsukka Southeast, Nigeria, and thus to determine the antibacterial resistance profile of the isolates. Isolation of enterococci was done using Slanetz-Bartley enterococci selective medium. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. From 95 swabs, 93 (97.89%) were positive for enterococci. Of the 93 isolates, 10 (10.75%) were haemolytic Enterococcus species, while 83 (89.25%) were non-haemolytic Enterococcus species. Out of 75 isolates, all (100%) were resistant to cefoxitin, 66 (88%) were resistant to ampicillin, 71 (94%) to amoxicillin/clavulanic acid, 68(90.7%) to ceftriaxone, 42 (56%) to streptomycin, 74 (98.67%) to gentamicin, 16 (21.3%) to tetracycline, 5 (6.7%) to vancomycin, 62 (82.7%) to sulphamethoxazole/trimethoprim, and 1 (1.3%) to chloramphenicol and ciprofloxacin. None of the isolate was resistant to imipenem. The enterococcal isolates exhibited 22 resistance patterns. Out of 75 isolates, 1 (1.3%) isolate was resistant to 1 class of antibacterial agents, 9 (12%) were resistant to 2 classes, and 65 (86.7%) to 3 or more classes. This study has shown that cattle slaughtered in Nsukka Southeast Nigeria are potential reservoirs and disseminators of multidrug-resistant enterococci.

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Microbiological investigation of Escherichia coli isolates from cloacal and feacal swabs of broiler chickens for extended spectrum beta lactamase (ESBL) enzymes

Abstract: Extended spectrum beta-lactamases (ESBLs)

Cited by 6 publications

References 11 publications

Distribution of TET, AAC and CTX-M Genes among Antibiotic Resistant Escherichia coli Isolated From Poultry under Various Farming System of A and N Islands

Distribution of TET, AAC and CTX-M Genes among Antibiotic Resistant Escherichia coli Isolated From Poultry under Various Farming System of A and N Islands

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Prevalence and Antibiogram of Generic Enterococci in Ready-to-Slaughter Beef Cattle

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