Microbiological evaluation of the steam sterilization of assembled laparoscopic instruments

Abstract

Objective:assess the safety of steam sterilization of assembled laparoscopic instruments with challenge contamination. Method:a laboratory experimental study, using as test samples trocars and laparoscopic graspers. Geobacillus stearothermophillus ATCC-7953 was used, with a microbial population of 106UFC/Filter paper substrate, removed from the biological indicator. Three of them were introduced into each instrument at the time of assembly, and sterilized at pressurized saturated steam, 134oC for 5 minutes. After sterilization, the instrument was disassembled and each filter paper substrate was inoculated in soybean casein culture and incubated at 56oC for 21 days. In case of absence of growth, they were subjected to heat shock of 80oC, for 20 minutes and re-incubated for 72 hours. Sample size: 185 graspers and 185 trocars, with 95% power. We paired the experiments with comparative negative control groups (5 graspers and 5 trocars with challenge contamination, sterilized disassembled) and positive control (30 filter paper supports, unsterilized), subject to the same incubation procedures. Results:there was no microbial growth in experimental and negative control. The results of the positive control were satisfactory. Conclusion:this study provided strong scientific evidence to support the safety of steam sterilizing of the assembled laparoscopic instrument.

Unravelling the mystery microorganism: a case study of chronic surgical site discharge post laparoscopic hysterectomy linked to Mycobacterium chelonae

“…While steam sterilization is a recommended method for sterilizing these instruments and has shown effectiveness, ethylene oxide treatment is also increasingly being used. [17][18][19][20] Despite the advantages of laparoscopic hysterectomy over invasive procedures, ensuring thorough sterilization of instruments before reuse is paramount. This case highlights the critical importance of adhering to proper sterilization protocols for laparoscopic instruments in hysterectomy procedures.…”

Port site infection by Mycobacterium fortuitum after laparoscopic hysterectomy and its management– A case report

“…One of the advised methods of sterilisation of laparoscopic instrument is steam sterilisation, which has showed good efficacy. 14 In recent days, laparoscopic instrument is subjected to ethylene oxide treatment for its sterilisation. 15 No doubt the laparoscopic hysterectomy has its advantages over the invasive hysterectomy.…”

Monitoramento e rastreabilidade de artigos esterilizados no bloco operatório

“…The effectiveness of the processing of articles through sterilization with the destruction of bacterial spores should be determined from the reading of the biological indicators that are used to monitor sterilization cycles. 20 The importance of reading the biological indicator after the incubation period related to its generation, identifying positivity or negativity of the microbial growth, since its technology is indispensable to guarantee security to the monitoring of the sterilization. 7,20 It was found in the results of this research with the multiprofessional team that only one (1%) participant reported that the biological indicator is used in the first sterilization cycle of the day and in all loads with implant materials, such as plaque and screws, and that the reading of the result is obtained in three hours, being verified the positivity or the negativity in the growth of microorganism.…”