Microbiological evaluation of acute periradicular abscesses by DNA-DNA hybridization

Siqueira, José F.; Rôças, Isabela N.; Souto, Renata; Uzeda, Milton de; Colombo, Ana Paula Vieira

doi:10.1067/moe.2001.118620

Cited by 94 publications

(100 citation statements)

References 35 publications

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“…Los microambientes de la cavidad oral permiten que un grupo de especies se establezcan en diferentes lugares, en diferentes cantidades y proporciones (1,4,6).…”

Section: Introductionunclassified

Identificación por reacción en cadena de la polimerasa (PCR) de microorganismos presentes en las infecciones orofaciales odontogénicas

Miranda

Peña

2014

Rev Estomatol Herediana.

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Identificación por reacción en cadena de la polimerasa (PCR) de microorganismos presentes en las infecciones orofaciales odontogé-nicasGhersi-Miranda HD, Inga-Peña RM. Identificación por reacción en cadena de la polimerasa (PCR) de microorganismos presentes en las infecciones orofaciales odontogénicas. Rev Estomatol Herediana. 2010; 20(1):5-12. RESUMENEl objetivo del presente trabajo fue identificar la presencia de las bacterias, Porphyromonas gingivalis, Tannerella forsythensis y Treponema denticola, denominado complejo rojo; en grupo de tres, en parejas o sola una especie, presentes en las infecciones orofaciales odontogénicas (IOFO), mediante el método de la Reacción de la Polimerasa Reversa (PCR), en las muestras de abscesos de los pacientes que acudieron al Servicio de Odontoestomatologia del Hospital Nacional Cayetano Heredia y a la Clínica Estomatológica Central de la Universidad Peruana Cayetano Heredia. Se obtuvieron 35 muestras, en ninguna de ellas se identificó el complejo bacteriano integrado por P. gingivalis, T. forsythensis y T. denticola. La asociación entre P. gingivalis y el T. forsythensis fue la más frecuente con un 5,71%. Al menos uno de los integrantes del complejo bacteriano estuvo presente en el 48,57% (17 de 35) muestras. La P. gingivalis es la bacteria más prevalente del complejo bacteriano con el 40% (14 de 35) muestras, seguido de la T. forsythensis en un 11,43% (4 de 35) muestras. La P. gingivalis se identificó en un 42,86% (6 de 14) muestras del género femenino y 38,1% (8 de 21) muestras del género femenino. Este estudio ha demostrado por medio de la prueba de PCR, la prevalencia de tres especies bacterianas presentes en las infecciones orofaciales odontogénicas. Aunque la etiología de estos procesos es multifactorial, es muy importante considerar los resultados de este estudio para el manejo racional de las infecciones.Palabras Clave: REACCIÓN EN CADENA DE LA POLIMERASA / Porphyromonas gingivalis / Treponema denticola. Identification of bacteria associated with orofacial infections by polimerase chain rection (PCR)ABSTRACT The objective of this investigation was to identify the prevalence of Porphyromonas gingivalis, Tannerella forsythensis and Treponema denticola forming the red complex, or as pairs or as single species in odontogenic infections, by mean of the Polymerase Chain Reaction (PCR). The samples were obtained from 35 oral and facial abscesses. In no case the red complex was found. The most frequent association found was P. gingivalis and T. forsythensis that represented 5.71%. At least, one specie was identified in 17 samples (48.57%). P. gingivalis was the most common bacteria representing 40% (14 out of 35 samples). T. forsythensis was identified in 11.43% (4 out of 35 samples). T. denticola was the less prevalent with only two cases (5.71%). P. gingivalis was demonstrated in 8 out of 21 males (38%) and in 6 out of 14 females (42.85%). This study has demonstrated by PCR the prevalence of three species of odontogenic bacteria as responsible of orofacial infections. Alth...

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“…Los microambientes de la cavidad oral permiten que un grupo de especies se establezcan en diferentes lugares, en diferentes cantidades y proporciones (1,4,6).…”

Section: Introductionunclassified

Identificación por reacción en cadena de la polimerasa (PCR) de microorganismos presentes en las infecciones orofaciales odontogénicas

Miranda

Peña

2014

Rev Estomatol Herediana.

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“…According to Siqueira et al, 21 E. coli is present in 3.7% of acute periapical abscesses, when evaluated by the checkerboard deoxyribonucleic acid (DNA)-DNA hybridization. Moreover, its endotoxin presents the basic structure of the lipid component, which represents the active center responsible for the toxicity of LPS.…”

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Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

Valera

Oliveira

Maekawa

et al. 2016

The Journal of Contemporary Dental Practice

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Aim:The purpose of this in vitro study was to evaluate the antimicrobial activity of 2% chlorhexidine gel (CHX) as auxiliary chemical substance and intracanal medications on Candida albicans, Enterococcus faecalis, Escherichia coli, and their endotoxins in the root canals. Materials and methods:The study was conducted on 48 single-rooted human teeth divided into four groups (n = 12), according to intracanal medications used: (1) Calcium hydroxide + apyrogenic saline solution (Ca(OH) 2 + SS), (2) 20% ginger glycolic extract (GEN), (3) calcium hydroxide + 20% ginger glycolic extract (Ca(OH) 2 + GEN), (4) apyrogenic SS (control). Collections were made from the root canal content before preparation (baseline-S1), immediately after instrumentation (S2), 7 days after instrumentation (S3), after 14 days the action of intracanal medication (S4), and 7 days after removal of the intracanal medication (S5). The antimicrobial activity and endotoxin content were analyzed for all collections. The results were statistically analyzed by the Kruskal-Wallis and Dunn tests at a significance level of 5%.Results: After instrumentation with CHX, there was complete elimination of E. coli and C. albicans, except for E. faecalis, which was significantly reduced and then completely eliminated after intracanal medication. There was significant reduction of endotoxin after instrumentation. of endotoxins in all groups; this reduction was greater in group Ca(OH) 2 followed by the group GEN. Conclusion:It was concluded that the instrumentation using CHX and intracanal medication used were able to eliminate the microorganisms from the root canal; the endotoxins were reduced, yet not completely eliminated. Clinical significance:This study is important and relevant for searching alternatives during endodontic therapy, since it aims to study the effect of Zingiber officinale on microorganisms and endotoxins present in root canals.

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“…Foi identificada a presença de S. aureus, por meio de PCR, em 11,1% dos canais radiculares de dentes com abscesso periodontal agudo (Siqueira Jr. et al, 2001). Em análise por PCR realizada por Jacinto et al (2007), foi verificada a presença de S. aureus em 2 amostras entre 5, de canais radiculares.…”

Section: Enterococcus E Streptococcusunclassified

“…A prevalência de E. faecalis é maior em infecções secundárias do que em infecções primárias, estando presente entre 24 a 77% dos casos de lesões periapicais persistentes, especialmente assintomáticas (Peculiene et al, 2001;Gomes et al, 2004;Rôças et al, 2004;Sen, 2004;Stuart et al, 2006;Sakamoto et al, 2008). S. aureus também é uma bactéria que oferece grande resistência ao tratamento endodôntico, estando presente tanto em infecções primárias como nas infecções persistentes dos canais radiculares (Siqueira Jr. et al, 2001;Jacinto et al, 2007;Tanomaru et al, 2008;Tirali et al, 2009). …”

Section: Da Metodologiaunclassified

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Efeitos in vitro de extratos naturais sobre Enterococcus faecalis, Staphylococcus aureus, Escherichia coli e endotoxinas em canais radiculares

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Microbiological evaluation of acute periradicular abscesses by DNA-DNA hybridization

Cited by 94 publications

References 35 publications

Identificación por reacción en cadena de la polimerasa (PCR) de microorganismos presentes en las infecciones orofaciales odontogénicas

Identificación por reacción en cadena de la polimerasa (PCR) de microorganismos presentes en las infecciones orofaciales odontogénicas

Action of Chlorhexidine, Zingiber officinale, and Calcium Hydroxide on Candida albicans, Enterococcus faecalis, Escherichia coli, and Endotoxin in the Root Canals

Efeitos in vitro de extratos naturais sobre Enterococcus faecalis, Staphylococcus aureus, Escherichia coli e endotoxinas em canais radiculares

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