Microbiological and molecular characterization of denitrification in biofilters treating pig manure

Gilbert, Yan; Bihan, Yann Le; Aubry, Geneviève; Veillette, Marc; Duchaine, Caroline; Lessard, Paul

doi:10.1016/j.biortech.2007.06.066

Cited by 25 publications

(23 citation statements)

References 33 publications

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“…BraulioVillalobos et al [27] obtained a 72% organic matter removal rate from an influent with 300 mg L −1 COD. Gilbert et al [28] also evaluated a trickling filter for treating wastewater from pig farms and observed a higher level of efficiency than that obtained in the present study, reducing COD content from 15,300 mg L −1 in the influent to 330 mg L −1 in the effluent. However, their trickling filter operated Figure 3 shows the changes in the dissolved oxygen concentration, which increased from 0.2 mg L −1 to an average of 3.1 mg L −1 .…”

Section: Discussioncontrasting

confidence: 38%

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Removing Organic Matter and Nutrients from Swine Wastewater after Anaerobic–Aerobic Treatment

Terán

Orozco

Acuña

et al. 2017

Water

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Anaerobic digesters generate effluent containing about 3000 mg L −1 of organic matter in terms of chemical oxygen demand (COD). This effluent must be treated before being reused or discharged into the environment. The objective of this study was to evaluate the efficiency of a trickling filter packed with red volcanic rock for the treatment of anaerobic digester effluent with COD concentrations of around 3000 mg L −1 . The trickling filter consisted of an aluminum cylinder, 2 mm thick, 3 m high, and 1 m in diameter. To evaluate the efficiency of the treatment system, there were three experimental runs, each lasting 20 days (d). The predictor variable was the initial COD concentration, which ranged from 2002 to 3074 mg L −1 . The hydraulic retention time was 9 h. The influent flow was 2.2 L min −1 , which amounts to a hydraulic load of 4033 m 3 m −2 day −1 and an organic load of 0.006342 to 0.009738 kg m −3 day −1 of COD. Independent of the initial concentration, COD removal efficiency was very high, varying from 90 to 96%. Final effluents met all the maximum permissible limits to be used as irrigation water, as well as for its release into natural or artificial water reservoirs, stored for agricultural crop irrigation.

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Section: Discussioncontrasting

confidence: 38%

“…Gilbert et al [28] obtained a removal efficiency of 75% for total nitrogen when treating pig wastewater with concentrations of 3200 mg L −1 , but with a very low hydraulic load. This study involved lower retention times and a lower nitrogen load (0.067 kg m −2 day −1 ) than those of our study (0.559 kg m −2 day −1 ).…”

Section: Discussionmentioning

confidence: 99%

Removing Organic Matter and Nutrients from Swine Wastewater after Anaerobic–Aerobic Treatment

Terán

Orozco

Acuña

et al. 2017

Water

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“…N 2 O, with a global warming potential approximately 296 times higher than that of carbon dioxide, is an important greenhouse gas [8]. The nosZ gene, encoding the catalytic subunit of N 2 O reductase, and the nirS/K gene, encoding nitrite reductase, are widely used as functional markers for studying the abundance of bacteria able to metabolize N 2 O and NO, to better understand the main causes of N 2 O and NO emissions [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…Real-time quantitative PCR (qPCR) is the most popular approach used to target the above functional genes to quantify the abundance of the functional groups [9,10,12]. However, an external standard or a calibrant is needed to quantify the environmental sample when using qPCR.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

Meng

Wang

et al. 2014

Anal Bioanal Chem

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DNA reference materials of certified value have a critical function in many analytical processes of DNA measurement. Quantification of amoA genes in ammonia oxidizing bacteria (AOB) and archaea (AOA), and of nirS and nosZ genes in the denitrifiers is very important for determining their distribution and abundance in the natural environment. A plasmid reference material containing nirS, nosZ, amoA-AOB, and amoA-AOA is developed to provide a DNA standard with copy number concentration for ensuring comparability and reliability of quantification of these genes. Droplet digital PCR (ddPCR) was evaluated for characterization of the plasmid reference material. The result revealed that restriction endonuclease digestion of plasmids can improve amplification efficiency and minimize the measurement bias of ddPCR. Compared with the conformation of the plasmid, the size of the DNA fragment containing the target sequence and the location of the restriction site relative to the target sequence are not significant factors affecting plasmid quantification by ddPCR. Liquid chromatography-isotope dilution mass spectrometry (LC-IDMS) was used to provide independent data for quantifying the plasmid reference material. The copy number concentration of the digested plasmid determined by ddPCR agreed well with that determined by LC-IDMS, improving both the accuracy and reliability of the plasmid reference material. The reference value, with its expanded uncertainty (k=2), of the plasmid reference material was determined to be (5.19±0.41)×10 9 copies μL −1 by averaging the results of two independent measurements. Consideration of the factors revealed in this study can improve the reliability and accuracy of ddPCR; thus, this method has the potential to accurately quantify DNA reference materials.

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“…The chemical reducing conditions in raw manure, shown as negative ORP values in Figure 4, benefited NO x -N removal when mixed with nitrified effluent due to the oxidation of the organic carbon using nitrogen oxidized species as an electron acceptor (Gilbert et al, 2008;Vanotti and Szogi, 2008). Heterotrophic microorganisms have a very high growing rate which favors denitrification processes (Potter et al, 1998;Ahn, 2006).…”

Section: Resultsmentioning

confidence: 99%

Nitrogen removal from swine wastewater by combining treated effluent with raw manure

Kunz

Steinmetz

Damasceno

et al. 2012

Sci. agric. (Piracicaba, Braz.)

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Effluents from swine raising can be harmful to the environment if not correctly managed. Nitrogen (N) is usually the main element present at high concentrations in the effluent. Since the use as biofertilizer is not always a feasible alternative, the treatment of swine wastewater is necessary. Variations in N species and water solubility make the treatment difficult and expensive. Additional N removal at low cost via denitrification may be possible by recirculating nitrified effluent in the barns. In this study, raw manure (RM) was homogenized with treated effluent (TE) at RM/(RM + TE) ratios of 1.0, 0.9, 0.8, 0.7, 0.6, 0.5 and 0 in order to simulate the effect of reused water on swine wastewater nitrogen removal. Samples were collected daily during four days and analyzed for pH, oxidation-reduction potential, NH 4 -N, NO 2 -N, NO 3 -N and chemical oxidation demand. The oxidized nitrogen (NO x -N) half-life degradation was estimated using linear regression. NO x -N species half-life less than one day was obtained when treated effluent was combined and thoroughly homogenized with raw manure. It is suggested that combining raw manure with treated effluent (e.g. water reuse) can be a simple and cost-effective strategy to remove nitrogen from swine wastewaters.

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Microbiological and molecular characterization of denitrification in biofilters treating pig manure

Cited by 25 publications

References 33 publications

Removing Organic Matter and Nutrients from Swine Wastewater after Anaerobic–Aerobic Treatment

Removing Organic Matter and Nutrients from Swine Wastewater after Anaerobic–Aerobic Treatment

Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers

Nitrogen removal from swine wastewater by combining treated effluent with raw manure

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