Microbiological and Epidemiological Characterization of Imipenem-Resistant<i>Pseudomonas aeruginosa</i>Strains from a Brazilian Tertiary Hospital: Report from the SENTRY Antimicrobial Surveillance Program

Ribeiro, Julival; Mendes, Rodrigo E.; Domingos, Renato Mateus; França, Emmanuel Rodrigues de; Silbert, Suzane; Jones, Ronald N.; Sader, Hélio S.

doi:10.1179/joc.2006.18.5.461

Cited by 13 publications

(9 citation statements)

References 19 publications

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“…Bacterial strains of P. aeruginosa resistant to most antimicrobials and sensitive only to PB have been isolated by clinical microbiology laboratories in most Brazilian hospitals [19,20]; this was corroborated by the results presented in this study. In a study over a period of 1 year in Mexico, P. aeruginosa was the third most frequently isolated species from blood in an intensive care unit (ICU) and the predominant isolate in central venous catheters among the species included in the ESKAPE group of multidrug-resistant microorganisms [21].…”

Section: Discussionsupporting

confidence: 76%

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

et al. 2014

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Background: Pseudomonas aeruginosa commonly causes nosocomial bloodstream infections and the emergence of a variety of β-lactamases (BLs) is worrying. In 5 hospitals in Belo Horizonte, Brazil, the presence of phenotypes encoding BL genes was established and the genetic diversity of the P. aeruginosa strains recovered from bloodstream infections was analyzed. Materials and Methods: The isolates were investigated using a disk diffusion (DD) method and the Etest®, for encoding metallo-β-lactamases (MBLs), oxacillinases and cephalosporinases. Genes and genetic diversity were evaluated by random amplified polymorphic DNA (RAPD) genotyping and enterobacterial repetitive intergenic consensus (ERIC)-PCR. Results: Twelve strains (30%) were positive for MBLs by Etest and DD, 15 were cephalosporinase-positive and 87.5% were positive for bla_SPM-1 and bla_VIM-1. Twenty-three strains (57.5%) were grouped into profile A, 32.5% into profile B and 10% into profile C by RAPD genotyping. ERIC-PCR revealed a varying degree of similarity between strains, ranging from 45 to 100%. Conclusions: The results suggest distinct clonal populations in the 5 hospitals studied, indicating a potentially problematic epidemiological situation in Belo Horizonte, Brazil.

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Section: Discussionsupporting

confidence: 76%

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

et al. 2014

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“…9 Data from the Sentry antimicrobial surveillance program documented increases in MDR in Latin America, 10 as have other microbiological studies. [11][12][13][14] Few studies have evaluated hospitals in respect to the epidemiology and trends of overall resistance of MDR GNB. 15 Steady increases of different types of phenomena of multidrug resistance are observed in day-to-day practice in our hospital due to the growing complexity of procedures.…”

Section: Introductionmentioning

confidence: 99%

Trends of 9,416 multidrug-resistant Gram-negative bacteria

Oliveira

Rubio

Almeida

et al. 2015

Rev. Assoc. Med. Bras.

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Conflict of interest: noneObjective: a resistance of hospital-acquired bacteria to multiple antibiotics is a major concern worldwide. The objective of this study was to investigate multidrugresistant (MDR) bacteria, clinical specimens, origin of specimen and trends, and correlate these with bacterial sensitivity and consumption of antimicrobials. Methods: 9,416 bacteria of nosocomial origin were evaluated in a tertiary hospital, from 1999 to 2008. MDR was defined for Gram-negative bacteria (GNB) as resistance to two or more classes/groups of antibiotics. Results: GNB MDR increased by 3.7 times over the study period (p<0.001). Acinetobacter baumannii was the most prevalent (36.2%). Over the study period, there were significant 4.8-fold and 14.6-fold increases for A. baumannii and K. pneumoniae (p<0.001), respectively. Sixty-seven percent of isolates of MDR GNB were isolated in intensive care units. The resistance of A. baumannii to carbapenems increased from 7.4 to 57.5% during the study period and concomitant with an increased consumption. Conclusion: that decade showed prevalence of GNB and a gradual increase in MDR GNB. There was an increase in carbapenem resistance of 50.1% during the study.

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“…porin mutations and efflux pumps) and/or carbapenem-hydrolyzing β-lactamases, such as metallo-β-lactamases (MβL) 3 . It has been suggested that in the absence of MβL, high imipenem resistance rates in isolates can show great genomic variability, which can be associated with continuous selection of resistant mutants 8 . However, in many geographic regions of Brazil, this has been associated with the dissemination of an epidemic clone that produces SPM MβL 9 .…”

Section: Introductionmentioning

confidence: 99%

Intrahospital spread of carbapenem-resistant Pseudomonas aeruginosa in a University Hospital in Florianópolis, Santa Catarina, Brazil

Scheffer

Bazzo

Steindel

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Introduction: Carbapenem-resistant Pseudomonas aeruginosa (CRPA) has been isolated with increasing frequency in Brazilian hospitals. Since June 2003, its detection in a teaching hospital in the city of Florianópolis, Brazil, has increased. This study aimed to investigate the minimal inhibitory concentration (MIC), presence of Metallo-β-lactamase (MβL) and a possible clonal relationship among the isolates. Methods: The study included 29 CRPA and seven isolates with reduced susceptibility. The MIC was determined by agar-dilution. Detection of MβL was performed by Double Disk Sinergism (DDS) and Combined Disk (CD). The MβL gene was verified by PCR and nucleotide sequence analysis. Epidemiological typing was performed by pulsed-field gel electrophoresis. Results: Among the 29 carbapenem-resistant isolates, polymyxin B presented 100% susceptibility and piperacillin/tazobactam 96.7%. Seventeen (62%) strains were verified as clonal (A clone) and among these, six isolates indicated phenotypically positive tests for MβL and harbored the bla SPM-1 gene. The first CRPA isolates were unrelated to clone A, harbored bla IMP-16 and were phenotypically positive only by CD. Conclusions: The spread of a high-level of resistance clone suggests cross transmission as an important dissemination mechanism and has contributed to the increased rate of resistance to carbapenems. This study emphasizes the need for continuous surveillance and improved strategies for infection control in this institution.

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Microbiological and Epidemiological Characterization of Imipenem-ResistantPseudomonas aeruginosaStrains from a Brazilian Tertiary Hospital: Report from the SENTRY Antimicrobial Surveillance Program

Cited by 13 publications

References 19 publications

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

Phenotypic and Genotypic Diversity of Multidrug-Resistant Pseudomonas aeruginosa Isolates from Bloodstream Infections Recovered in the Hospitals of Belo Horizonte, Brazil

Trends of 9,416 multidrug-resistant Gram-negative bacteria

Intrahospital spread of carbapenem-resistant Pseudomonas aeruginosa in a University Hospital in Florianópolis, Santa Catarina, Brazil

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