Microbially influenced corrosion of carbon steel in the presence of anaerobic sulphate-reducing bacteria

Černoušek, Tomáš; Shrestha, Rojina; Kovářová, Hana; Špánek, Roman; Ševců, Alena; Sihelská, Kristína; Kokinda, Jakub; Stoulil, J.; Steinová, Jana

doi:10.1080/1478422x.2019.1700642

Cited by 22 publications

(13 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The extracted DNA was used to describe the microbial composition in the samples (16S rDNA amplicon sequencing, NGS) and for the relative quantification of microbial biomass in the samples (16S rDNA quantitative PCR). For these DNA-based analyses, protocols similar to [34] were followed.…”

Section: Microbiological Characterisationmentioning

confidence: 99%

Geochemical, Geotechnical, and Microbiological Changes in Mg/Ca Bentonite after Thermal Loading at 150 °C

et al. 2021

View full text Add to dashboard Cite

Bentonite buffers at temperatures beyond 100 °C could reduce the amount of high-level radioactive waste in a deep geological repository. However, it is necessary to demonstrate that the buffer surrounding the canisters withstands such elevated temperatures, while maintaining its safety functions (regarding long-term performance). For this reason, an experiment with thermal loading of bentonite powder at 150 °C was arranged. The paper presents changes that the Czech Mg/Ca bentonite underwent during heating for one year. These changes were examined by X-ray diffraction (XRD), thermal analysis with evolved gas analysis (TA-EGA), aqueous leachates, Cs sorption, cation exchange capacity (CEC), specific surface area (SSA), free swelling, saturated hydraulic conductivity, water retention curves (WRC), quantitative polymerase chain reaction (qPCR), and next-generation sequencing (NGS). It was concluded that montmorillonite was partially altered, in terms of the magnitude of the surface charge density of montmorillonite particles, based on the measurement interpretations of CEC, SSA, and Cs sorption. Montmorillonite alteration towards low- or non-swelling clay structures corresponded well to significantly lower swelling ability and water uptake ability, and higher saturated hydraulic conductivity of thermally loaded samples. Microbial survivability decreased with the thermal loading time, but it was not completely diminished, even in samples heated for one year.

show abstract

Section: Microbiological Characterisationmentioning

confidence: 99%

Geochemical, Geotechnical, and Microbiological Changes in Mg/Ca Bentonite after Thermal Loading at 150 °C

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The goal was to provide in situ corrosion rates and insights regarding the viability and persistence of microbes in bentonite. Of particular interest are sulfate-reducing bacteria (SRB), whose activity has been shown to lead to microbiologically influenced corrosion (MIC) of carbon steel ( Černoušek et al, 2020 ). It is expected that a considerable number of bacterial cells will remain viable despite the harsh conditions expected post-repository closure, which include increased pressure, heat, and irradiation ( Haynes et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Growth and Persistence of an Aerobic Microbial Community in Wyoming Bentonite MX-80 Despite Anoxic in situ Conditions

et al. 2022

View full text Add to dashboard Cite

Microbial activity has the potential to enhance the corrosion of high-level radioactive waste disposal canisters, which, in the proposed Swiss deep geological repository, will be embedded in bentonite and placed in the Opalinus Clay (OPA) rock formation. A total of 12 stainless steel cylindrical vessels (referred to as modules) containing bentonite were deployed in an anoxic borehole in OPA for up to 5.5 years. Carbon steel coupons were embedded in the bentonite. Individual modules were retrieved after 1, 1.5, 2.5, and 5.5 years. Enumeration of aerobic and anaerobic heterotrophs and sulfate-reducing bacteria (SRB) revealed microbial growth for 1.5 years followed by a decline or stagnation in microbial viability. It was surprising to observe the growth of aerobic heterotrophs followed by their persistent viability in bentonite, despite the nominally anoxic conditions. In contrast, SRB numbers remained at very low levels. DNA-based amplicon sequencing confirmed the persistence of aerobes and the relatively low contribution of anaerobes to the bentonite microbiome. Bentonite dry density, in situ exposure time, and bioavailable trapped oxygen are observed to shape the bentonite microbial community in the clay.

show abstract

“…Uniform corrosion will usually entail through SRB, which may Fig. 1 Various factors influence corrosion versus biocorrosion-here, we show the formation of the microbial biofilm involved in the biocorrosion process form oxides of their metabolic processes and further enhance the rates of corrosion [17][18][19].…”

Section: Metal Corrosionmentioning

confidence: 79%

Analytical Characterisation of Material Corrosion by Biofilms

Dang

Power²,

Cozzolino

et al. 2022

J Bio Tribo Corros

View full text Add to dashboard Cite

Almost every abiotic surface of a material is readily colonised by bacteria, algae, and fungi, contributing to the degradation processes of materials. Both biocorrosion and microbially influenced corrosion (MIC) refer to the interaction of microbial cells and their metabolic products, such as exopolymeric substances (EPS), with an abiotic surface. Therefore, biofouling and biodeterioration of manufactured goods have economic and environmental ramifications for the user to tackle or remove the issue. While MIC is typically applied to metallic materials, newly developed and evolving materials frequently succumb to the effects of corrosion, resulting in a range of chemical reactions and transport mechanisms occurring in the material. Recent research on biocorrosion and biofouling of conventional and novel materials is discussed in this paper, showcasing the current knowledge regarding microbial and material interactions that contribute to biocorrosion and biofouling, including biofilms, anaerobic and aerobic environments, microbial assault, and the various roles microorganisms’ play. Additionally, we show the latest analytical techniques used to characterise and identify MIC on materials using a borescope, thermal imaging, Fourier transform infrared (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM), X-ray photoelectron microscopy (XPS), X-ray diffraction (XRD), optical and epifluorescence microscopy, electrochemical impedance spectroscopy, and mass spectrometry, and chemometrics.

show abstract

Microbially influenced corrosion of carbon steel in the presence of anaerobic sulphate-reducing bacteria

Cited by 22 publications

References 53 publications

Geochemical, Geotechnical, and Microbiological Changes in Mg/Ca Bentonite after Thermal Loading at 150 °C

Geochemical, Geotechnical, and Microbiological Changes in Mg/Ca Bentonite after Thermal Loading at 150 °C

Growth and Persistence of an Aerobic Microbial Community in Wyoming Bentonite MX-80 Despite Anoxic in situ Conditions

Analytical Characterisation of Material Corrosion by Biofilms

Contact Info

Product

Resources

About