Microbial valorization of oil-based substrates for polyhydroxyalkanoates (PHA) production – Current strategies, status, and perspectives

Lim, Sook Wei; Kansedo, Jibrail; Tan, Inn Shi; Tan, Yie Hua; Nandong, Jobrun; Lam, Man Kee; Ongkudon, Clarence M.

doi:10.1016/j.procbio.2023.05.013

Cited by 9 publications

(3 citation statements)

References 193 publications

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“…A modified strain of C. necator is able to utilize crude corn (maize) starch to produce 63% PHB [65]. The use of oils for PHA production has recently been reviewed by [112]. Palm oil, despite its controversial production, can also be used as a source, and C. necator B-10646 can accumulate up to 72% PHA [113].…”

Section: Food Productsmentioning

confidence: 99%

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Fukala,

Kučera

2024

Molecules

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Polyhydroxyalkanoates (PHAs) are intracellular biopolymers that microorganisms use for energy and carbon storage. They are mechanically similar to petrochemical plastics when chemically extracted, but are completely biodegradable. While they have potential as a replacement for petrochemical plastics, their high production cost using traditional carbon sources remains a significant challenge. One potential solution is to modify heterotrophic PHA-producing strains to utilize alternative carbon sources. An alternative approach is to utilize methylotrophic or autotrophic strains. This article provides an overview of bacterial strains employed for PHA production, with a particular focus on those exhibiting the highest PHA content in dry cell mass. The strains are organized according to their carbon source utilization, encompassing autotrophy (utilizing CO2, CO) and methylotrophy (utilizing reduced single-carbon substrates) to heterotrophy (utilizing more traditional and alternative substrates).

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Section: Food Productsmentioning

confidence: 99%

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Fukala,

Kučera

2024

Molecules

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“…This increased reactivity has resulted in significant attention being paid to the chemical epoxidation reaction, which is a common and ecologically favourable reaction in industry. 21,22 Many vegetable oils are mixtures composed of unsaturated triglycerides, which can be chemically converted into epoxide derivatives using various synthetic routes; such vegetable oil epoxidation is an appealing process as the epoxidized vegetable oils can be employed as biobased building blocks for the preparation of polyhydroxyalkanoates 23–25 and non-isocyanate polyurethanes (NIPUs). 26–30…”

Section: Introductionmentioning

confidence: 99%

Amino acids as bio-organocatalysts in ring-opening copolymerization for eco-friendly synthesis of biobased oligomers from vegetable oils

Werlinger,

Beroíza-Duhart,

Douglas-Gallardo

et al. 2024

Org. Biomol. Chem.

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“…Plant oils are suitable precursors for PHA production, as they provide more carbon units than sugars and are located downstream of sugars in the PHA production route, making them easier to utilize [ 12 , 13 ]. In addition, plant oils can provide precursors of 3-3HHx via phaJ , which is an effective carbon source for P(3HB- co -3HHx) production.…”

Section: Introductionmentioning

confidence: 99%

Identification of Oil-Loving Cupriavidus necator BM3-1 for Polyhydroxyalkanoate Production and Assessing Contribution of Exopolysaccharide for Vegetable Oil Utilization

Shin,

Kim,

Choi

et al. 2024

Polymers

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Polyhydroxyalkanoates (PHA) have received attention owing to their biodegradability and biocompatibility, with studies exploring PHA-producing bacterial strains. As vegetable oil provides carbon and monomer precursors for poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (P(3HB-co-3HHx)), oil-utilizing strains may facilitate PHA production. Herein, Cupriavidus necator BM3-1, which produces 11.1 g/L of PHB with 5% vegetable oil, was selected among various novel Cupriavidus necator strains. This strain exhibited higher preference for vegetable oils over sugars, with soybean oil and tryptone determined to be optimal sources for PHA production. BM3-1 produced 33.9 g/L of exopolysaccharides (EPS), which was three-fold higher than the amount produced by H16 (10.1 g/L). EPS exhibited 59.7% of emulsification activity (EI24), higher than that of SDS and of EPS from H16 with soybean oil. To evaluate P(3HB-co-3HHx) production from soybean oil, BM3-1 was engineered with P(3HB-co-3HHx) biosynthetic genes (phaCRa, phaARe, and phaJPa). BM3-1/pPhaCJ produced 3.5 mol% of 3HHx and 37.1 g/L PHA. BM3-1/pCB81 (phaCAJ) produced 32.8 g/L PHA, including 5.9 mol% 3HHx. Physical and thermal analyses revealed that P(3HB-co-5.9 mol% 3HHx) was better than PHB. Collectively, we identified a novel strain with high vegetable oil utilization capacity for the production of EPS, with the option to engineer the strain for P(3HB-co-3HHx).

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Microbial valorization of oil-based substrates for polyhydroxyalkanoates (PHA) production – Current strategies, status, and perspectives

Cited by 9 publications

References 193 publications

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Natural Polyhydroxyalkanoates—An Overview of Bacterial Production Methods

Amino acids as bio-organocatalysts in ring-opening copolymerization for eco-friendly synthesis of biobased oligomers from vegetable oils

Identification of Oil-Loving Cupriavidus necator BM3-1 for Polyhydroxyalkanoate Production and Assessing Contribution of Exopolysaccharide for Vegetable Oil Utilization

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